Uncontrolled use of antibiotics in the treatment of calves suffering from gastroenteritis and dairy cows suffering from mastitis, created in pathogenic staphylococci enterotoxin producers some resistance to these drugs. These pathogens enterotoxin producers are often excreted with milk from cows with mastitis. That is why the specific and topical issue remains the isolation and identification of strains producing staphylococcal enterotoxin from cow milk and faeces of calves suffering from enteritis. Given all this, we have set a goal to get staphylococcal enterotoxin type D for use as mastitis diagnostic kit and enteritis and antigen for obtaining the antiserum. To obtain the staphylococcal enterotoxin type D we used strain S. aureus No. 494 (ATSS23235) courtesy offered by Dr. FC Fluer from Institute of Experimental Medicine, NF Gamaley, Medical Academy (USSR). Verification of the specificity of the enterotoxin) was performed using 5 strains of S.aureus producing different enterotoxins (entérotoxin- A; enterotoxin-B; enterotoxin C-1; C-2 and enterotoxin - E) as well as non-enterotoxigenic staphylococcal strain (S. aureus Wood 46). In addition to the staphylococcal strains, five strains of Escherichia coli, producing thermostable and thermolabile enterotoxins were also used in the experiment. The method of purification of enterotoxin-D by precipitation twice chromatography on km - cellulose and gel filtration on Sephadex G-75 allows to obtain pure enterotoxin-D having the biological properties, immunological and antigenic characteristics. Immunization of rabbits with purified enterotoxin-D associated with incomplete Freund's adjuvant provides antiserum very active and specific.
Ban-Bo Bebanto Antipas,
Podkapaev Vladimir Michaelovich,
Purification of Staphylococcal Enterotoxin Type D and Obtaining Its Antiserum, Animal and Veterinary Sciences.
Vol. 3, No. 6,
2015, pp. 166-170.
Alikaev VA, VV Matuchin. Fight against calves gastroenteritis. Veterinariya, 1982, 4, 51-55.
Alikaev VA. Prophylaxis of cow diseases in industrial livestock complexes. Moscow kolos, 1976, 192p.
Abzaloba AG. Mastitis and gastroenteritis. Uralski Nivi, 1978, 3, 52-53.
Bidjeh K. Development of identification method of staphylococcal enterotoxin type D in milk /colostrum of cows and in the faecal samples of calves suffering from enteritis. phD thesis, Veterinary Academy of Moscow KI Skriyabin, Moscow, 1984, 176p.
Baïliozov D. B., Panaïtova M., Visiolinov V.O. About the identification method of staphylococcal enterotoxin. JMEI, 1962, 8, 101-109.
Bougrova V.I., Nikolaeva I.S. obtaining the enterotoxin- A for the diagnosis of staphylococcal intoxication. Vapros pitani, 1974.6, 47-52.
Berdoll MS. The nature of staphylococcal enterotoxins. Clinical toxicol. 1972, 5, 4, 441.
Berdoll MS, Huang IY, Schantz EJ. Chemistry of staphylococcal entertoxins. J. Agric. Food Chem., 1974, 22, 9-13.
Winduga Bozena. Occurrence of enterotoxigenic staphylococci especially producors of entertoxin- D and E among Strains isolated from food. Acta food. Pol., 1978, 4, 2, 129-132.
Kartachova V.M. Fast detection method of staphylococcal toxin in milk and dairy products. Veterinariya, 1970, 11, 105-107.
Kartachova V.M. Methods for identifying staphylococci and their toxins in milk and milk products. In: Identification of pathogenic bacteries in milk and milk products, Moscow, Kolos, 1973, 108-148.
Kuchko I.V., Xatmever M.L., Akatov A.K. About the possibility of the method of use of part of the intestine ligature of experimental animals for identification of enterotoxins. In: The problem of staphylococcal infections. Tch. I. Saratov, 1979, 13-15.
Minor T.E., Mart E.X. Staphylocci in food products, Moscow, 1980. 231p.
Niskanen A., Korainen L., Roine K. Staphylococcal enterotoxin and Thermonuclease production during the induced bovine mastitis and the clinical reaction of enterotoxin in Udders. Infection and Immunity, 1978, 19, 2, 493-498.
Sabri Darvich Morgan Selim. Immunochemical Method identification of the staphylococci producing enterotoxin-A isolated from cows with mastitis. phD thesis, 1979 178, 178p.
Santos E.C., Moreira H.A. Influence of mastitis induced by staphylococcal enterotoxin protein on the composition of bovine milk. Arg. E sc. Vet., 1976, 28, 3, 227-232.
Santos E.C., Moreira H.A. Influence of staphylococcal mastitis induced by enterotoxin on production of bovine milk. Arg. E sc. Vet., 1977, 29, 1, 5-10.
Slavchev G. Economic importance of sub/clinical mastitis in cows. Vet. Sb., 1982, 80, 8, 34-36.
Stolmakova A.I. Food poisoning original staphylococci. University of Lvov, Lvov, 1959. 22p.
Stolmakova A.I. Experimental poisoning of animals by the staphylococcal enterotoxin, 1954, 13, 5, 28-33.
Fluer F.S. Some physicochemical, structural and functional properties of staphylococcal entertoxins. JMEI, 19, 80, 12, 9-17.
Mutovin V.I. Fight against mastitis of cows, Moscow, 1974, 254p.
Nourmatov B.A., Dvites NE. Bacteriological diagnostic of staphylococcal infections (methodological Recommendations) Tashkent, 1978, 22p.
Mateveev K.I. Annals of microbiological diagnosis of infectious diseases, 2nded, Moscow, 1973, 624p.
Akatov A.K., Zueva V.S. Staphylococci, Medicine, Moscow, 1983, 256p.
Casman E.P. Serological studies of staphylococcal Enterotoxins. Public health, report, 1958, 73, 599-609.
Casman E.P, Berdoll M.S., Robinson J. Designation of staphylococcal enterotoxins. J. Bacteriol., 1963, 85, 715-716.
Davis B.J. Disc electrophoresis. II. Method and Application to human proteins. Ann. NY Acad. Science 1964, 121, 404-427.
Jarvis A.W. and Lowrence R.C. Production of high titers of Enterotoxins for the routine testing of staphylococci. Applied Microbiology, 1970, 19, 4, 698-699.
Noleto A.L. and Berdoll M.S. Staphylococcal enterotoxin Production in the non-enterotoxigenic staphylococci. Applied and Environmental Microbiology, 1980, 39, 6, 1167-1171.
Donnelly C.B., leslie J.E., Black LA. Serological identification of enterotoxigenic staphylococci in cheese. Applied Microbiology, 1967, 15, 1382-1387.
Barto P.B., Bush L.J., Adams G.D. Effect of sour colostrum on survival of Staphylococcus aureus. Animal Science Research report, 1979.104, 265-326.
Dolman E.C., Wilson R G. The kitten test for staphylococcal enterotoxin. Canadian Journal of Public Health, 1940, 31, 68-71.
Hammon Mcd. W. Staphylococcal enterotoxin: An Improved cat test. Chemical and immunological studies. Am. J. Public Health, 1941, 31, 1191-1198.
Kato Tsuneo E. and Kume. Enterotoxigenicity of staphylococci isolated from bovine mastitis California test- positive milking. Japan. J. Vet. Res. 1980, 28, 3, 75-85.
Olson J.C., Casman E.P., Berdoll M.S. Enterotoxigenicity cultures of staphylococcus aureus isolated from bovine mastitis of acute cases. Journal of applied microbiology, 1970, 20, 4, 605- 607.
Ostachevski A.G., Obrassov V.P., Kotenko I.I. Using the precipitation reaction for the identification of enterotoxigenic staphylococci. Trudi kharkov, zoovet., Institute., 1970 (1971), 5, 29, 22-26.
Ouchterlony. Antigen antibodies reactions in gels. Acta Patholol. Microbiol. Scand., 1949, 25, 507-515.
Reisfeld R.A., Lewis U.J., Williams D.E. Disc electrophoresis of proteins in basic polyacrylamid gels. Nature 1962, 195, 281-283.
Volovenko M.A. Effect of colostrum and milk products coming from cows with mastitis on natural resistance and morbidity of calves. PhD Thesis, Kharkov, 1975 173P.
Venskiyavischen V.B. Effect of udder on the morbidity of calves. Bulletin of the Scientific and Technological Information, 1967.1, 46-47.
Favourite G.O., Hammon W.M. Production of staphylococcal toxin, enterotoxin and alpha hemolysin in a simplified medium. Journal of bacteriology, 194,141, 305-316.
Berdoll M.S. Staphylococcal poisoning. In: H. Riemann and Bryan F.L. (ed) Food borne infections and intoxications 2nd ed. Academic Press, New York, 1979, 443-494.
Berdoll MS, H. Sugiyama, Dack GM. Staphylococcal enterotoxin I. Purification. Ach. Bioch. Biophys., 1959, 85, 85-89.
Bozena Z.S. Enterotoxigenicity of staphylococci isolated from cow milk with mastitis. Medecin veterinariya 1980, 36, 6, 368-370.
Casman E.P., Bennet R.W., Dorsey A.E. Issa J.A. Identification of staphylococcal enterotoxin D, fourth serotype. Bacteriology, 1967, 94, 6, 1875-1882.
Chang H.C. and Berdoll M.S. Purification of Reduced and Some properties of staphylococcal enterotoxin-D. Biochemistry 1979, 18, 1937-1942.
Graber P. Immunoelectrophoretic analysis. Methods Biochem. 1959 7, 1-38
Shinagawa K., N. Kunita Simplified methods for purification of staphylococcal Enterotoxins A and C and preparation of anti – enterotoxin. Jap. Bacterial J. 1975, 30, 6, 683-692.
Tingting zhang, Anning Huang, Junnian Li, Liu Xinqin, Liuli, Lu Yao, Ying Huang and Wang WinPing. Characterization of antimicrobial resistance and enterotoxin in meticillin - resistant Staphylococcus aureus isolated from milk and food poisoning cases. Journal of animal and veterinary advances, 201413 (7) 423-429.