Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration
Animal and Veterinary Sciences
Volume 7, Issue 5, September 2019, Pages: 112-120
Received: Aug. 21, 2019;
Accepted: Sep. 4, 2019;
Published: Nov. 13, 2019
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Kamel Mohammed, Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Gamal Darwish, Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Ragab Dohreig, Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Ahmed Mahmoud, Cattle Production Department, Armed Force Farm, Fayoum, Egypt
Zaher Rawash, Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Abdel Khaleik Nassra, Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Magdy Badr, Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Ayman Hassan, Animal Biology Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
Sayed Essawy, Animal Biology Department, Animal Reproduction Research Institute (ARRI), Giza, Egypt
The study was investigated to compare the effectiveness of Folltropin and two products (Fostimon and Menogon) of a recombinant human follicle stimulating hormone (rhFSH) on ovarian super-stimulation and embryo production in dairy Holstein cows. Two different doses for each super-ovulatory product were investigated. The relationship between the levels of Anti-Mullerian Hormone (AMH) in the blood serum before super-stimulation initiation and ovarian response and embryos production were also evaluated. At random stage of the estrus cycle, the donor cows were received intra-vaginal controlled internal drug release device (CIDR) and injected with 100μg of gonadotropin-releasing hormone (GnRH). On fourth day of CIDR insertion, cows were divided according to the product types of superovulatory hormones into three equal main groups. According to hormonal dose, each main group was re-divided into two equally sub-groups; low and high dose. Superovulatory hormones was injected IM as decreasing doses; 40%, 30%, 20% and 10% of the total dose twice daily for 4 days. On day 7, CIDRs were removed and each cow received 2 doses of PGF2α with 12hr interval. Based upon heat detection, donor's cows were inseminated with frozen semen three times 8-10hrs interval and concurrent with 100μg GnRH injection at first insemination. Non-surgical embryos recovery was performed on day's 7-8 post insemination and ovarian super-stimulation was checked using sonography to estimate ovarian response. Recovered embryos were morphologically evaluated as grades. Twelve embryos collected from Menogon donors group were transferred to six recipient's cows. The mean numbers of CL, recovered embryo/ova, fertilized ova and transferable embryos are significantly (P<0.01) higher in Folltropin and Menogon treated cows than Fostimon group. Donor cows treated with high doses of Folltropin and Menogon showed higher means for transferable and freezable embryos. Three recipients (50%) who received embryos appeared pregnant and one of them born calf. Significant positive correlations were reported between AMH on one side and CL, total ovarian response, transferable and freezable embryos on the other side, and cows having AMH≥230ng/L exhibited significant higher means. In conclusion: Menogon can be used as rhFSH for embryos production with transferable quality. AMH can be used as indicator to helpful select cow's candidates as donors. More studies are needed using Fostimon accompanied with different levels of LH.
Abdel Khaleik Nassra,
Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration, Animal and Veterinary Sciences. Special Issue: Application of Advanced Reproductive Technologies for Production Transferable Embryos.
Vol. 7, No. 5,
2019, pp. 112-120.
Choudhary KK, Kavya KM, Jerome A, Sharma RK. Advances in reproductive biotechnologies. Vet World 2016; 9 (4): 388–395.
Gibbons A, Pereyra Bonnet F, Cueto MI, Catala M, Salamone DF, Gonzalez-Bulnes A. Procedure for maximizing oocyte harvest for in vitro embryo production in small ruminants. Reprod Domest Anim 2007; 42: 423-426.
Menchaca A, Vilarino M, Crispo M, de Castro T, Rubianes E. New approaches to superovulation and embryo transfer in small ruminants. Reprod Fertil Dev 2010; 22: 113-118.
Mapletoft RJ, Steward KB, Gregg P, Adams A. Recent advances in the superovulation in cattle. Reprod Nutr Dev 2002; 42: 601–611.
Quaresma MA, Lopes da Costa L, Robalo Silva J. Superovulation of Mertolenga cows with two FSH preparations (FSH-P and FOLLTROPIN). Rev Port Ci Vet 2003; 98 (546): 81-84.
Ferré L, Bogliotti Y, Chitwood J, Kjelland M, Ross P. Hormonal follicle stimulation in Holstein cows for in vitro embryo production using sperm sorted by flow cytometry. Reprod Fert Dev 2016; 28: 248.
Tasdemir U, Karasahin T, Satilmis M, Hamdi kizil S, Kaymaz M. Various FSH Administration on Superovulatory Response and Embryo Yield in Anatolian Black Heifers. Kocatepe Vet J 2016; 9 (4): 322-326.
Mikkola M, Taponen J. Embryo yield in dairy cattle after superovulation with Folltropin or Pluset. Theriogenology 2017; 15 (88): 84-88.
Giuliana Dell’Eva, Davide Bolognini, Eleonora Iacono, Barbara Merlo. Superovulation protocols for dairy cows bred with SexedULTRA™ sex‐sorted semen. Reprod Dom Anim 2019; 54: 756–761.
Dias FC, Khan MI, Adams GP, Sirard MA, Singh J. Granulosa cell function and oocyte competence: Super-follicles, super-moms and super-stimulation in cattle. Anim Reprod Sci 2014; 149 (1–2): 80-89.
Marian O, Manuela P, Alexandru S, Alin I, Birþoiu. The Effect of Epidural Administration of FSH in Bovine Superovulation Protocol. Anim Sci 2015; LVIII: 217-220.
Zangirolamo AF, Morotti F, Silva NC, Sanches TK, Seneda MM. Ovarian antral follicle populations and embryo production in cattle. Proceedings of the 34rd Meeting of the Association of Embryo Transfer in Europe (AETE); Nantes, France, September 7th and 8th, 2018.
Hirayama H, Naito A, Fujii T, Sugimoto M, Takedomi T, Moriyasu S, Sakai H, Kageyama S. Effects of genetic background on responses to superovulation in Japanese Black cattle. J Vet Med Sci 2019; 81 (3): 373–378.
Simopoulou M, Sfakianoudis K, Antoniou N, Maziotis E, Rapani A, Bakas P, Anifandis G, Kalampokas T, Bolaris S, Pantou A, Pantos K, Koutsilieris M. Making IVF more effective through the evolution of prediction models: is prognosis the missing piece of the puzzle? Syst Biol Reprod Med 2018; 64 (5): 305-323.
Keith Center, Dave Dixon, Charles Looney, Rick Rorie. Anti-Mullerian Hormone and Follicle Counts as Predictors of Superovulatory Response and Embryo Production in Beef Cattle. Advan Reprod Sci 2018; 6: 22-33.
Monniaux D, Drouilhet L, Rico C, Estienne A, Jarrier P, Touzé JL, Sapa J, Phocas F, Dupont J, Dalbiès-Tran R et al. Regulation of antiMüllerian hormone production in domestic animals. Reprod Fert Devel 2012; 25: 1–16.
Monniaux D, Barbey S, Rico C, Fabre S, Gallard Y, Larroque H. Anti-Mullerian hormone: a predictive marker of embryo production in cattle? Reprod Fertil Dev 2010; 22: 1083-1091.
Rico C, Drouilhet L, Salvetti P, Dalbiès-Tran R, Jarrier P, Touzé JL, Pillet E, Ponsart C, Fabre S, Monniaux D. Determination of anti-Müllerian hormone concentrations in blood as a tool to select Holstein donor cows for embryo production: from the laboratory to the farm. Reprod Fert Dev 2012; 24: 932–944.
Cliff Lamb G, Stevenson JS, KeslerJ, Garverick HA, Brown DR, Salfen BE. Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F for ovulation control in postpartum suckled beef cows. J. Anim. Sci 2001; 79: 2253-2259.
Larson JE, Lamb GC, Stevenson JS, Johnson SK, Day ML, Geary TW, Kesler DJ, DeJarnette JM, Schrick FN, DiCostanzo A, Arseneau JD. Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination and timed artificial insemination using gonadotropin-releasing hormone, prostaglandin F2α, and progesterone (CIDR): J Anim Sci 2006; 84 (2): 332-342.
Robertson I, Nelson R. Certification and identification of the embryo. In: Stringfellow DA, Seidel SM (eds), Manual of the International Embryo Transfer Society, 3rd edition. Savoy, IL; IETS, 1998; 103-134.
Bo G, Mapletoft R. Evaluation and classification of bovine embryos. Anim Reprod 2013; 54: 344–348.
Singh J, Dominguez M, Jaiswal R, Adams GP. A simple ultrasound test to predict the superstimulatory response in cattle. Theriogenology 2004; 62: 227–243.
Ireland JJ, Ward F, Jimenez-Krassel F, Ireland JL, Smith GW, Lonergan P, Evans AC. Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle. Hum Reprod 2007; 22: 1687-1695.
Silva JC, Alvarez RH, Zanenga CA, Pereira GT. Factors affecting embryo production in superovulated Nelore cattle. Anim Reprod 2009, 6 (3): 440-445.
Ireland JJ, Smith GW, Scheetz D, Jimenez-Krassel F, Folger JK, Ireland JL, Mossa F, Lonergan P, Evans AC. Does size matter in females? An overview of the impact of the high variation in the ovarian reserve on ovarian function and fertility, utility of anti-Mullerian hormone as a diagnostic marker for fertility and causes of variation in the ovarian reserve in cattle. Reprod Fertil Dev 2011; 23: 1-14.
Evans AC, Mossa F, Walsh SW, Scheetz D, Jimenez-Krassel F, Ireland JL, Smith GW, Ireland JJ. Effects of maternal environment during gestation on ovarian folliculogenesis and consequences for fertility in bovine offspring. Reprod Domest Anim 2012; 47 (4): 31-37.
Jaton C, Koeck A, Sargolzaei M, Malchiodi F, Price CA, Schenkel FS, Miglior F. Genetic analysis of superovulatory response of Holstein cows in Canada. J Dairy Sci 2016; 99 (5): 3612-3623.
Parker Gaddis KL, Dikmen S, Null DJ, Cole JB, Hansen PJ. Evaluation of genetic components in traits related to superovulation, in vitro fertilization, and embryo transfer in Holstein cattle. J Dairy Sci 2017; 100 (4): 2877-2891.
Moore SG, Cummins SB, Mamo S, Lonergan P, Butler ST. Genetic merit for fertility traits in Holstein cows: VI. Oocyte developmental competence and embryo development. J Dairy Sci 2019; 102 (2): 4651-4661.
Palubinskas G, Zˇilaitis G, Antanaitis R. Improvement of dairy cow embryo yield with low level laser irradiation. Polish J Vet Sci 2017; 20 (2): 307–312.
Chupin D, Combarnous Y, Procureur R. Antagonistic effect of LH in commercially available gonadotrophins. Theriogenology 1984; 25: 167.
Tasdemir U, Satilmis M, Karasahin T, Hamdi Kizil S, Kaymaz M, Imai K. The Effect of Single Epidural Plus Intramusculer Injection of FSH on Superovulatory Response in Anatolian Black Cow. Ankara Üniv Vet Fak Derg 2012; 59: 211-216.
Filicori M, Cognigni GE, Pocognoli P, Tabarelli C, Ferlini F, Perri T, Parmegiani L. Comparison of controlled ovarian stimulation with human menopausal gonadotropin or recombinant follicle-stimulating hormone. Fertil Steril 2003; 80 (2): 390-397.
Wilson JM, Jones AL, Moore K, Looney CR, Bondioli KR. Superovulation of cattle with a recombinant-DNA bovine follicle stimulating hormone. Anim Reprod Sci 1993; 33: 71–82.
Takagi M, Kim IH, Izadyar F, Hyttel P, Bevers MM, Dieleman SJ, Hendriksen PJ, Vos PL. Impaired final follicular maturation in heifer after superovulation with recombinant human FSH. Reproduction 2001; 121 (6): 941-51.
Viudes-de-Castro MP, Pomares A, M. Saenz de Juano MD, Marco-Jiménez F, Vicente JS. Effect of luteinizing hormone on rabbit ovarian superstimulation and embryo developmental potential. Theriogenology 2015; 84 (3): 446-451.
Hattori K, Orisaka M, Fukuda S, Tajima K, Yamazaki Y, Mizutani T, Yoshida Y. Luteinizing Hormone Facilitates Antral Follicular Maturation and Survival via Thecal Paracrine Signaling in Cattle. Endocrinology 2018; 159 (6): 2337-2347.
Paulo D. Carvalho, Katherine S. Hackbart, Robb W. Bender, Giovanni M. Baez, Paul M. Fricke. Use of a single injection of long-acting recombinant bovine FSH to superovulate Holstein heifers: A preliminary study. Theriogenology 2014; 82 (3): 481-489.
Kanitz W, Becker F, Schneider F, Kanitz E, Leiding C, Nohner HP, Pöhland R. Superovulation in cattle: practical aspects of gonadotropin treatment and insemination. Reprod Nutr Dev 2002; 42 (6): 587-599.
Willmott N, Saunders J, Bo GA, Palasz A, Pierson RA, Mapletoft RJ. The effect of FSH/LH ratio in pituitary extracts on superovulatory response in the cow. Theriogenology 1990; 33: 347.
Cliff Lamb. Factors affecting an embryo transfer program. In proceedings: Applied Reproductive Strategies in Beef Cattle 2005; Lexington, Kentucky.
Gonzalez A, Lussier JG, Carruthers TD, Murphy BD, Mapletoft RJ. Superovulation of beef heifers with Folltropin. A new FSH preparation containing reduced LH activity. Theriogenology 1990; 33: 519–529.
Hiroshi Kishi, Yoshikazu Kitahara, Fumiharu Imai, Kohshiro Nakao, Hiroto Suwa.Expression of the gonadotropin receptors during follicular development. Reprod Med Biol 2018; 17 (1): 11–19.
Greve T, Callesen H, Hyttel P, Hoier R, Assey R. Effects of exogenous gonadotrophins in oocyte and embryo quality in cattle. Theriogenology 1995; 43: 41-50.
Kumar P, Farouk SS. Luteinizing hormone and its dilemma in ovulation induction. J Hum Reprod Sci 2011; 4 (1): 2–7.
Wiley C, Jahnke M, Redifer C, Gunn PJ, Dohlman T. Effects of endogenous progesterone during ovarian follicle superstimulation on embryo quality and quantity in beef cows. Theriogenology 2019; 4 (15) 129: 54-60.
Ali MS, Khandoker MA, Afroz MA, Bhuiyan AK. Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle. Asian Australas J Anim Sci 2012; 25 (1): 52–58.
Alkemade SJ, Murphy BD, Mapletoft RJ. Superovulation in the cow: Effects of biological activity of gonadotropins, Proc 12th Ann. Conv. AETA, Portland, Maine 1993.
Barati F, Niasari-Naslaji A, Bolourchi M, Sarhaddi F, Razavi K, Naghzali E, Thatcher WW. 2006. Superovulatory response of Sistani cattle to three different doses of FSH during winter and summer. Theriogenology 2006; 66 (5): 1149-55.
Anderson RC, Newton CL, Anderson RA, Millar RP. Gonadotropins and their analogs: Current and Potential Clinical Applications. Endocr Rev 2018; 39 (6): 911-937.
Borgbo T, Klučková H, Macek M, Chrudimska J, Kristensen SG, Hansen LL, Andersen CY. The Common Follicle-Stimulating Hormone Receptor (FSHR) Promoter Polymorphism FSHR −29G > A Affects Androgen Production in Normal Human Small Antral Follicles. Front Endocr (Lausanne) 2017; 2 (8): 122.
Laven JS. Follicle Stimulating Hormone Receptor (FSHR) Polymorphisms and Polycystic Ovary Syndrome (PCOS). Front Endocr (Lausanne) 2019; 12 (10): 23.
Batista EO, Macedo GG, Sala RV, Ortolan MDDV, Sá Filho MF, Del Valle TA, Jesus EF, Lopes RN, Rennó FP, Baruselli PS. Plasma antimullerian hormone as a predictor of ovarian antral follicular population in Bos indicus (Nelore) and Bos taurus (Holstein) heifers. Reprod Domest Anim 2014; 49: 448-452.
Souza AH, Carvalho PD, Rozner AE, Vieira LM, Hackbart KS, Bender RW, Dresch AR, Verstegen JP, Shaver RD, Wiltbank MC. Relationship between circulating anti-Müllerian hormone (AMH) and superovulatory response of high-producing dairy cows. J Dairy Sci 2015; 98: 169–178.
Hirayama H., Naito A, Fukuda S, Fujii T, Asada M, Inaba Y, Takedomi T, Kawamata M, Moriyasu S, Kageyama S. Long-term changes in plasma anti-Müllerian hormone concentration and the relationship with superovulatory response in Japanese Black cattle. J Reprod Dev 2017; 63: 95–100.
Visser JA, de Jong FH, Laven JS, Themmen AP. Anti-Mullerian hormone: a new marker for ovarian function. Reprod 2006; 131: 1-9.
Baruselli PS, Batista EO, Vieira LM, Souza AH. Relationship between follicle population, AMH concentration and fertility in cattle. Anim Reprod 2015; 12 (3): 487-497.
Nabenishi H, Kitahara G, Takagi S, Osawa T. Relationship between plasma anti-Müllerian hormone concentrations during the rearing period and subsequent embryo productivity in Japanese black cattle. Domest Anim Endocr 2017; 60: 19-24.
Redhead AK, Siew N, Lambie N, Carnarvon D, Knights M. The relationship between circulating concentration of AMH and LH content in the follicle stimulating hormone (FSH) preparations on follicular growth and ovulatory response to superovulation in water buffaloes. Anim Reprod Sci 2018; 188 (1): 66-73.
Stojsin-Carter A, Mahboubi K, Costa NN, Gillis DJ, Carter TF, Neal MS, Miranda MS, Ohashi OM, Favetta LA, King WA. Systemic and local anti-Mullerian hormone reflects differences in the reproduction potential of Zebu and European type cattle. Anim Reprod Sci 2016; 167: 51-58.
Visser JA, Themme AP. Role of anti-Müllerian hormone and bone morphogenetic proteins in the regulation of FSH sensitivity. Mol Cell Endocr 2014; 382 (1): 460-465.
Silberstein T, MacLaughlin DT, Shai I, Trimarchi JR, Lambert-Messerlian G, Seifer DB, Keefe D, Blazar AS. Müllerian inhibiting substance levels at the time of HCG administration in IVF cycles predict both ovarian reserve and embryo morphology. Hum reprod 2006; 21: 159-163. European type cattle. Animal Reproduction Science 167, 51-58. STOJSIN-CARTER A., MAHBOUBI K., COSTA N. N., GILLIS D. J., CARTER T. F., NEATakagi M, Kim IH, Izadyar F, Hyttel P, Bevers MM, Dieleman SJ, Hendriksen PJ, Vos PL. Impaired final follicular maturation in heifer after superovulation with recombinant human FSH. Reproduction 2001; 121 (6): 941-51.