Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs
Animal and Veterinary Sciences
Volume 6, Issue 2, March 2018, Pages: 21-26
Received: Apr. 6, 2018; Accepted: Apr. 23, 2018; Published: May 15, 2018
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Fábio Alessandro Pieri, Basic Life Sciences Department, Life Sciences Institute, Federal University of Juiz de Fora, Governador Valadares, Brazil
Vitor de Oliveira Silva, Veterinary Department, Health and Biological Sciences Center, Federal University of Viçosa, Viçosa, Brazil
Abelardo Silva Junior, Veterinary Department, Health and Biological Sciences Center, Federal University of Viçosa, Viçosa, Brazil
Maria Aparecida Scatamburlo Moreira, Veterinary Department, Health and Biological Sciences Center, Federal University of Viçosa, Viçosa, Brazil
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Periodontal disease, which affects the support structures and protection of teeth, is the main disease found in dogs, reaching about 85% prevalence in animals over 4 years old. The most important bacteria in the initial colonization of human dental plaque are Streptococcus, which produce exopolysaccharides that facilitate fixation of the bacteria to the surfaces. Some studies have sought to identify the dental plaque microbiota in dogs using non-selective culture media, and have reported small amounts of Streptococcus, however they did not provide ideal conditions for the growth of this bacteria. The aim of this study was to isolate and identify the initial dental plaque microbiota of dogs cultivable in a selective medium for Streptococcus, and verify the presence of this genus. Genotypic identification of the isolates were carried out by partial 16S rDNA sequencing. A total of 179 strains were obtained from the plaque of 10 dogs without periodontal disease. The technique employed allowed 15 different microbial groups to be isolated, including the genus Streptococcus. This represented 33 (18.4%) of 179 isolates, thus illustrating the partially selective ability of Mitis Salivarius Agar for samples of dental plaque in dogs.
Dogs, Oral Microbiology, Dental Plaque, Microbiota, Bacteria, 16S rDNA
To cite this article
Fábio Alessandro Pieri, Vitor de Oliveira Silva, Abelardo Silva Junior, Maria Aparecida Scatamburlo Moreira, Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs, Animal and Veterinary Sciences. Vol. 6, No. 2, 2018, pp. 21-26. doi: 10.11648/j.avs.20180602.11
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Kyllar, M. and Witter, K. (2005). Prevalence of dental disorders in pet dogs. Veterinary Medicine 11:496–505.
Niemiec, B. A. (2008). Periodontal therapy. Topics in Companion Animals Medicine 23:81-90.
Rosan, B. and Lamont, R. J. (2000). Dental plaque formation. Microbes Infection 2:1599-1607.
Komori, R., Sato, T., Takano-Yamamoto, T. and Takahashi, N. (2012). Microbial composition of dental plaque microflora on first molars with orthodontic bands and brackets, and the acidogenic potential of these bacteria. Journal of Oral Bioscience 54:107-112.
Pieri, F. A., Daibert, A. P., Bourguignon, E., Moreira, M. A. S. (2012). Periodontal disease in dogs. In: Perez-Marin, C. C. A Bird’s-Eye View of Veterinary Medicine. InTech, Zagreb, Croatia, pp: 119:140.
Elliott, D. R., Wilson, M., Buckley, C. M. and Spratt, D. A. (2005). Cultivable oral microbiota of domestic dogs. Journal of Clinical Microbiology 43:5470-5476.
Fonseca, S. A. D., Galera, P. D., Brito, D. L., Perecmanis, S., Silva, A. S., Cardoso, L. B., Marçola, T. G., Drummond, V. O. and Pimentel, C. M. (2011). Análise microbiológica da placa bacteriana da doença periodontal em cães e o efeito da antibioticoterapia sobre ela. Ciência Rural 41:1424-1429.
Riggio, M. P., Lennon, A., Taylor, D. J. and Bennett, D. (2011). Molecular identification of bacteria associated with canine periodontal disease. Veterinary Microbiology, 150:394-400.
Holcombe, L. J., Patel, N., Colyer, A., Deusch, O., O’Flynn, C. and Harris, s. (2014). Early Canine Plaque Biofilms: Characterization of Key Bacterial Interactions Involved in Initial Colonization of Enamel. PLOS one, 9:e113744.
Takada, K., Hayashi, K., Sasaki, K., Sato T. and Hirasawa, M. (2006). Selectivity of Mitis Salivarius agar and a new selective medium for oral streptococci in dogs. Journal of Microbiological Methods, 66:460-465.
Smith, A. C. and Hussey, M. A. (2013). Gram stain protocols. In: ACM Microbelibrary - Laboratory protocols. American Society for Microbiology, Washington, USA, Available in:
Reiner, K. (2013). Catalase test protocol. In: ACM Microbelibrary - Laboratory protocols. American Society for Microbiology, Washington, USA, Available in:
Sterr, Y., Weiss, A., and Schmidt, H; (2009). Evaluation of lactic acid bacteria for sourdough fermentation of amaranth. International Journal of Food Microbiology 136:75-82.
Sanger, F., Nicklen, S. and Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of National Academy of Science of United States of America 74:5463-5467.
Forney, L. J., Zhou X. and Brown, C. J. (2004). Molecular microbial ecology: land of the one-eyed king. Current Opinion in Microbiology 7:210-220.
Bisgaard, M. and Mutters, R. (1986). Characterization of Some Previously Unclassified Pasteurella spp Obtained from the Oral Cavity of Dogs and Cats and Description of a New Species Tentatively Classified with the Family Pasteurellaceae Pohl-1981 and Provisionally Called Taxon-16. Acta Pathologica, Microbiologica et Imunologica Scandinavica B 94:177-184.
Vancanneyt, M., Devriese, L. A., Graef, E. M., Baele, M., Lefebvre, K., Snauwaert, C., Vandamme, P., Swings J. and Haesebrouck, F. (2004). Streptococcus minor sp. nov., from faecal samples and tonsils of domestic animals. International Journal of Systematic Evolutional Microbiology 54:449-452.
Iijima, D. (2009). The Distribution of Genus Streptococcus in the Oral Cavities of Cats. International Journal of Oral-Medical Science 8:19-23.
Dent, V. E. and Marsh, P. D. (1981). Evidence for a basic plaque microbial community on the tooth surface in animals. Archieves of Oral Biology 26:171-179.
Rober, M., Quirynen, M., Haffajee, A. D., Schepers E. and Teughels, W. (2008). Intra-oral microbial profiles of beagle dogs assessed by checkerboard DNA-DNA hybridization using human probes. Veterinary Microbiology 127:79-88.
Fox, G. E., Wisotzkey J. D. and Jurtshuk P. Jr. (1992). How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. International Journal Systematic Evolutionary Microbiology 42:166-170.
Kim, S. E., Hwang, S. Y., Jeong, M., Lee, Y., Lee, E. R., Park, Y. W., Ahn, J. S., Kim, S. and Seo, K. (2016). Clinical and microbiological effects of a subantimicrobial dose of oral doxycycline on periodontitis in dogs. The Veterinary Journal 208: 55-59.
Zambori, C., Morvay, A. A., Sala, C., Licker, M., Gurban, C., Tanasie, G. and Tirziu, E. (2016). Antimicrobial effect of probiotics on bacterial species from dental plaque. The Journal of Infection in Developing Countries 10:214-221.
Jeussete, I. C., Román, A. M., Torre, C., Crusafont, J., Sánchez, N., Sánchez, M. C., Péres-Salcedo, L. and Herrera, D. (2016). 24-hour evaluation of dental plaque bacteria and halitosis after consumption of a single placebo or dental treat by dogs. American Journal of Veterinary Research 77:613-619.
Nowroozilarki, N., Jamshidi, S., Zalehi, T. Z. and Kolahian, S. (2017) Identification of Helicobacter and Wolinella spp. in Oral Cavity of Toy Breed Dogs With Periodontal Disease. Topics in Compannion Animals Medicine 32:96-99.
Paster, B. J., Boches, S. K., Galvin, J. L., Ericson, R. E., Lau, C. N., Levanos, V. A., Sahasrabudhe A. and Dewhirst, F. E. (2001). Bacterial diversity in human subgingival plaque. Journal of Bacteriology 183:3770-3783.
Socransky, S. S. and Haffajee, A. D. (2005). Periodontal microbial ecology. Periodontology 2000 38:135-187.
Lindinger, M. I. (2016). Reduced Dental Plaque Formation in Dogs Drinking a Solution Containing Natural Antimicrobial Herbal Enzymes and Organic Matcha Green Tea. Scientifica 2016: ID2183623.
Pieri, F. A., Souza, M. C. C., Vermelho, L. L. R., Vermelho, M. L. R., Perciano, P. G., Vargas, F. S., Borges, A. P. B., Veiga-Junior, V. F., and Moreira, M. A. S. (2016). Use of β-caryophyllene to combat bacterial dental plaque formation in dogs. BMC Veterinary Research 12:216.
Pieri, F. A., Silva, V. O., Vargas, F. S., Veiga-Junior, V. F. and Moreira, M. A. S. (2014) Antimicrobial activity of Copaifera langsdorffii oil and evaluation of its most bioactive fraction against bacteria of dogs dental plaque. Pakistan Veterinary Journal 34:165-169.
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