Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks
Animal and Veterinary Sciences
Volume 1, Issue 2, March 2013, Pages: 18-22
Received: Mar. 7, 2013;
Published: Mar. 10, 2013
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Sunanda Sharma, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Rajasthan University of Veterinary & Animal Sciences, Bikaner-334001, Rajasthan, India
Narendra K. Sharma, Divisional Mobile Veterinary Surgery and Infertility Unit, Animal husbandry Department, Government of Rajasthan, Bikaner, India
N. K. Sinha, Former Principal Scientist (Animal Reproduction), Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh, India
S. S. Sharma, Former Professor and Head, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Rajasthan University of Veterinary & Animal Sciences, Bikaner-334001, Rajasthan, India
Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructose-glycerol extender 1:4) and thawed (400C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 250C up to 50C) and equilibrated (at 50C for 2 hours) semen straws were frozen @ 15, 20, 25 and 300C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tran-ferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 109 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.
Narendra K. Sharma,
N. K. Sinha,
S. S. Sharma,
Assessment of Transaminases and Effect of Freezing Rates on Their Leakage into Seminal Plasma of Sirohi Bucks, Animal and Veterinary Sciences.
Vol. 1, No. 2,
2013, pp. 18-22.
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