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Response of Triticum aestivum (L.) Plants Grown Under Cadmium Stress to Polyamines Pretreatments
Volume 4, Issue 5, September 2016, Pages: 29-36
Received: Sep. 13, 2016; Accepted: Sep. 22, 2016; Published: Oct. 11, 2016
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Mostafa Mohamed Rady, Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Mohamed Ahmed Seif El-Yazal, Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Hanan Anwar Aly Taie, Plant Biochemistry Department, National Research Centre, Cairo, Egypt
Safia Mahmoud Abdel-Mageed Ahmed, Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
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The role of exogenously-applied polyamines [i.e., spermine (Spm), spermidine (Spd) and putrescine (Put)] in the improvement of cadmium (Cd2+) tolerance in wheat plants, and their effects on growth, yield and its components and changes in the osmoprotectant and endogenous Cd2+ concentrations and the contents of some nutrients in plants grown under 2.0 mM Cd2+ stress were assessed. The efficiency of wheat plants to tolerate Cd2+ stress in terms of growth and yield characteristics was noticed to varying degrees with the three applied polyamines. The reasonable growth of Cd2+-stressed seedlings and consequently acceptable grain yield was correlated with the improvements in the concentrations of osmoprotectants and tissue health in terms of relative water content (RWC) and membrane stability index (MSI), and reductions in electrolyte leakage (EL) and tissue Cd2+ concentration. Results show that, seed soaking in 0.25 mM Spm, 0.50 mM Spd or 1.0 mM Put generated significant better growth and yield characteristics, MSI, RWC, leaf photosynthetic pigment and osmoprotectant concentrations, and nutrient contents than seed soaking with water under 2.0 mM Cd2+ stress. In contrast, the Cd2+ concentration and EL were significantly reduced. However, the Cd2+-free control was the best treatment when compared to the all other stressed treatments. Seed soaking in 1.0 mM Put was the best, generating wheat plants that most tolerant to Cd2+ stress than those generated from the other two polyamines. Therefore, this study recommend to use the 1.0 mM Put, as seed soaking treatment for wheat to grow well under Cd2+ stress.
Wheat, Cadmium, Polyamines, Osmoprotectants, Growth, Yield
To cite this article
Mostafa Mohamed Rady, Mohamed Ahmed Seif El-Yazal, Hanan Anwar Aly Taie, Safia Mahmoud Abdel-Mageed Ahmed, Response of Triticum aestivum (L.) Plants Grown Under Cadmium Stress to Polyamines Pretreatments, Plant. Vol. 4, No. 5, 2016, pp. 29-36. doi: 10.11648/j.plant.20160405.11
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E. Zaki, M. Nabila, M. S. Hassanein, K. Gamal El-Din, "Growth and yield of some wheat cultivars irrigated with saline water in newly cultivated land as affected by biofertilization," J. Appl. Sci. Res., 3: 1121–1126, 2007.
M. D. Dewdar, M. A. El-Yazal, S. S. El-Ganaini, "Effect of biofertilization and optimization of nitrogen fertilizer on vegetative growth, chemical composition, yield and yield components trails of wheat plants," Egypt. J. Appl. Sci., 23: 486–501, 2008.
M. Hassan, S. Mansoor, "Oxidative stress and antioxidant defense mechanism in mung bean seedlings after lead and cadmium treatments," Turk. J. Agric. For., 38: 55–61, 2014.
K. Ozbek, N. Cebel, I. Unver, "Extractability and phytoavailability of cadmium in Cd-rich pedogenic soils," Turk. J. Agric. For., 38: 70–9, 2014.
M. Abdullah, M. Fasola, A. Muhammad, S. A. Malik, N. Boston, H. Bokhari, M. A. Kamran, M. N. Shafqat, M. Khan, N. Ali, S. A. M. Eqani, "Avian feathers as a non-destructive bio-monitoring tool of trace metals signatures: a case study from severely contaminated areas," Chemosphere, 119: 553–561, 2015.
S. Hayat, M. Alyemeni, S. Hasan, "Foliar spray of brassinosteroid enhances yield and quality of Solanum lycopersicum under cadmium stress," Saudi J. Biol. Sci., 19: 325–335, 2012.
Q. Wang, X. Liang, Y. Dong, L. Xu, X. Zhang, J. Hou, Z. Fan, "Effects of exogenous nitric oxide on cadmium toxicity, element contents and antioxidative system in perennial ryegrass," Plant Growth Regul., 69: 11–20, 2013a.
M. I. R. Khan, N. A. Khan, "Ethylene reverses photosynthetic inhibition by nickel and zinc in mustard through changes in PS II activity, photosynthetic nitrogen use efficiency, and antioxidant metabolism," Protoplasma, 251: 1007–1019, 2014.
V. B. Nagati, R. Koyyati, P. Marx, V. D. Chinnapaka, P. R. M. Padigya, "Effect of heavy metals on seed germination and plant growth on Grass pea plant (Lathyrus sativus)," Int. J. PharmTech Res., 7: 528–534, 2015.
M. A. Kamran, J. H. Syed, S. A. Eqani, M. F. Munis, H. J. Chaudhary, "Effect of plant growth-promoting rhizobacteria inoculation on cadmium uptake by Eruca sativa," Environ. Sci. Pollut. Res., 22: 9275–9283, 2015.
M. M. Rady, "Effect of 24-epibrassinolide on growth, yield, antioxidant system and cadmium content of bean (Phaseolus vulgaris L.) plants under salinity and cadmium stress," Sci. Hortic., 129: 232–237, 2011.
F. Cao, Y. Cai, L. Liu, M. Zhang, X. He, G. Zhang, F. Wu, "Differences in photosynthesis, yield and grain cadmium accumulation as affected by exogenous cadmium and glutathione in the two rice genotypes," Plant Growth Regul., 75: 715–723, 2015.
H. F. Farooq, H. N. Asghar, M. Y. Khan, M. Saleem, Z. A. Zahir, "Auxin-mediated growth of rice in cadmium-contaminated soil," Turk. J. Agric. For., 39: 272–276, 2015.
M. M. Rady, Kh. A. Hemida, "Modulation of cadmium toxicity and enhancing cadmium-tolerance in wheat seedlings by exogenous application of polyamines," Ecotoxicol. Environ. Saf., 119: 178–185, 2015.
R. Amooaghaie, "Role of polyamines in the tolerance of soybean to water deficit stress," World Acad. Sci., Eng. Technol., 80: 498–502, 2011.
H. M. Wallace, A. V. Fraser, A. Hughes, "A perspective of polyamine metabolism," Biochem. J., 376: 1–14, 2003.
H. Aldesuquy, H. Samia, A. Samy, E. Abdel-Whab, "Involvement of spermine and spermidine in the control of productivity and biochemical aspects of yielded grains of wheat plants irrigated with waste water," Egypt. J. Basic Appl. Sci., 1: 16–28, 2014.
J. Shi, X. Z. Fu, T. Peng, Q. Fan, J. H. Liu, "Spermine pretreatment confers dehydration tolerance of citrus in vitro plants via modulation of antioxidative capacity and stomatal response," Tree Physiol., 30: 914–922, 2010.
S. S. Hussain, M. Ali, M. Ahmad, K. H. M. Siddique, "Polyamines: Natural and engineered abiotic and biotic stress tolerance in plants," Biotechnol. Adv., 29: 300–311, 2011.
P. Sathe Atul, G. Paserkar Neha, B. Thakre Mahes, M. Gaikwad Sharad, "Engineering polyamines for abiotic stress tolerance," Ind. J. Appl. Res., 5: 1–25, 2015.
D. R. Hoagland, D. I. Arnon, "The Water Culture Method for Growing Plants Without Soil," CA Agric. Exp. St., Berkeley, CA, USA (Circular no. 374), 1950.
H. K. Lichtenthaler, A. R. Wellburn, "Determination of total carotenoids and chlorophyll a and b of leaf extract in different solvents," Biochem. Soc. Transact., 11: 591–592, 1983.
S. Hayat, B. Ali, S. A. Hasan, A. Ahmad, "Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea," Environ. Exp. Bot., 60: 33–41, 2007.
C. Y. Sullivan, W. M. Ross, "Selecting the drought and heat resistance in grain sorghum, In: H. Mussel, R. C. Staples (Eds), Stress Physiology in Crop Plants," John Wiley & Sons, New York, USA, pp. 263–281, 1979.
C. S. Piper, "Soil and plant analysis," New York, Interscience Publishers, Inc., Nc., USA, 1947.
M. L. Jakson, "Soil Chemical Analysis," Prentice Hall of India Privatelimited, New Delhi, India, 1967.
A. I. Page, R. H. Miller, D. R. Keeny, "Methods of Soil Analysis, Part II. Chemical and Microbiological Methods," second ed., Amer. Soc. Agron., Madison, WI, USA, pp. 225–246, 1982.
A. Hafez, D. S. Mikkelsen, "Colorimetric determination of nitrogen for evaluating the nutritional status of rice," Commun. Soil Sci. Plant Anal., 12: 61–69, 1981.
L. S. Bates, R. P. Waldeen, I. D. Teare, "Rapid determination of free proline for water stress studies," Plant Soil, 39: 205–207, 1973.
J. Jayarman, "Laboratory Manual in Biochemistry," Will Eastern limited, New York, 61–73, 1981.
S. M. Howladar, "A novel Moringa oleifera leaf extract can mitigate the stress effects of salinity and cadmium in bean (Phaseolus vulgaris L.) plants," Ecotoxicol. Environ. Saf., 100: 69–75, 2014.
W. M. Semida, M. M. Rady, T. A. Abd El-Mageed, S. M. Howladar, M. T. Abdelhamid, "Alleviation of cadmium toxicity in common bean (Phaseolus vulgaris L.) plants by the exogenous application of salicylic acid," J. Hortic. Sci. Biotechnol., 90: 83–91, 2015.
S. Choudhury, S. K. Panda, "Role of salicylic in regulating cadmium induced oxidative stress in Oryza sativa roots," Bulg. J. Plant Physiol., 30: 95–110, 2004.
M. Wang, Q. Zheng, Q. Shen, S. Guo, "The Critical Role of Potassium in Plant Stress Response – a review," Int. J. Mol. Sci., 14: 7370–7390, 2013b.
H. Marschner, "Mineral Nutrition of Higher Plants," second ed., Acad. Press Publ., NY, USA, pp. 559–579, 1995.
S. De Maria, M. Puschenreiter, A. R. Rivelli, "Cadmium accumulation and physiological response of sunflower plants to Cd during the vegetative growing cycle," Plant Soil Environ., 59: 254–261, 2013.
J. Kubis, J. Floryszak-Wieczorek, M. Arasimowicz-Jelonek, "Polyamines induce adaptive responses in water deficit stressed cucumber roots," J. Plant Res., 127: 151–158, 2014.
M. D. Groppa, M. P. Ianuzzo, M. L. Tomaro, M. P. Benavides, "Polyamine metabolism in sunflower plants under long-term cadmium or copper stress," Amino Acids, 32: 265–275, 2007a.
M. D. Groppa, M. L. Tomaro, M. P. Benavides, "Polyamines and heavy metal stress: the antioxidant behavior of spermine in cadmium- and copper-treated wheat leaves," BioMetals, 20: 185–195, 2007b.
M. R. Metwali, S. M. H. Gowayed, O. A. Al-Maghrabi, Y. Y. Mosleh, "Evaluation of toxic effect of copper and cadmium on growth, physiological traits and protein profile of wheat (Triticum aestivum L.), maize (Zea mays L.) and sorghum (Sorghum bicolor L.)," World Appl. Sci. J., 21: 301–314, 2013.
P. Ahmad, G. Nabi, M. Ashraf, "Cadmium-induced oxidative damage in mustard [Brassica juncea (L.) Czern. And Coss.] plants can be alleviated by salicylic acid," S. Afr. J. Bot., 77: 36–44, 2011.
P. Aravind, M. N. V. Prasad, "Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate–glutathione cycle and glutathione metabolism," Plant Physiol. Biochem., 43: 107–116, 2005.
S. S. Sharma, K. J. Dietz, "The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress," J. Exp. Bot., 57: 711–726, 2006.
P. Sharmila, P. Pardha Saradhi, "Proline accumulation in heavy metal stressed plants: An adaptive strategy, In: M. N.V. Prasad, K. Strzalka, K. (Eds), Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants," pp. 179–199, 2002.
H. J. Rhee, E. J. Kim, J. K. Lee, "Physiological polyamines: simple primordial stress molecules," J. Cell Mol. Med., 11: 685–703, 2007.
M. D. Groppa, M. P. Benavides, "Polyamines and abiotic stress: recent advances," Amino Acids, 34: 35–45, 2008.
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