Volume 5, Issue 5-1, October 2017, Pages: 13-18
Received: Jul. 7, 2016;
Accepted: Oct. 9, 2016;
Published: Oct. 18, 2016
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Md. Motiar Rahman, Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh
Md. Sahab Uddin, Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Md. Rashed Nejum, Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh
S. M. S. Al Din, Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh
G. M. Sala Uddin, Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh; Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Infectious diseases are one of the foremost causes of mortality and morbidity. In addition, day by day infection causing microbes are becoming resistant and modifying themselves into multi-drug resistant strains. So there is a crucial need to introduce new antibiotics to fight against these pathogens. The synthetic antibiotics are effective, but unsafe as well as are not consistent to use owing to the resistance building capacity of the microbes. Consequently, there is greater need for alternative treatments. Medicinal plants are store house of various types of phytochemicals and considered as a potential therapeutic remedy for numerous ailment. Therefore the aim of the present study was to evaluate the antibacterial activity of the leaves extract of Cajanus cajan (C. cajan) L. against coliform bacteria collected from tannery, tobacco and sugar mill waste water. In this study fresh leaves of C. cajan was extracted by using ethyl acetate, chloroform and n-Hexane. The antibacterial activity of these plant extracts was determined by measuring zone of inhibition (ZI) using the Agar disk diffusion method and minimum inhibitory concentration (MIC) was determined by using the microbroth dilution method. Among three extracts highest (25±0.18 mm, ZI) antibacterial activity was reported by ethyl acetate extract of C. cajan leaves against coliform bacteria isolated from sugar mill waste water at the highest concentration with respect to chloroform and n-Hexane extracts. In addition, antibacterial activity was also higher for ethyl acetate extract against coliform bacteria isolated from tannery and tobacco waste water compared to remaining extracts. The MIC of the ethyl acetate extract ranged 550 to 570 µg/ml. Therefore ethyl acetate extract of C. cajan leaves could be used as antibacterial agents against diseases caused by coliforms.
Md. Motiar Rahman,
Md. Sahab Uddin,
Md. Rashed Nejum,
S. M. S. Al Din,
G. M. Sala Uddin,
Study on Antibacterial Activity of Cajanus cajan L. Against Coliforms Isolated from Industrial Waste Water in Bangladesh, Plant. Special Issue: Phytotherapy.
Vol. 5, No. 5-1,
2017, pp. 13-18.
M. F. Hasan, M. A. Iqbal, M. S. Uddin. “Antibacterial and antifungal activity of Litsea monopetala Leaves on Selected Pathogenic Strains”. European J Med Plants, 2016; 12 (4): 1-8.
M. S. Uddin, A. A. Mamun, M. A. Iqbal, A. Islam, M. F. Hossain, S. Khanum, et al., “Analyzing nootropic effect of Phyllanthus reticulates Poir. on cognitive functions, brain antioxidant enzymes and acetylcholinesterase activity against aluminium-induced Alzheimer’s model in rats: Applicable for controlling the risk factors of Alzheimer’s Disease”. Adv Alzheimer’s Dis, 2016; 5: 87-102.
M. S. Uddin, A. A. Mamun, S. Khanum, Y. Begum, M. S. Alam. “Analysis of in vitro antioxidant activity of Caryota urens L. leaves: A traditional natural remedy”. J Coast Life Med, 2016, 4: 483-484.
B. C. Freeman, G. A. Beattie. “An overview of plant defenses against pathogens and herbivores”. Plant Hea Ins, 2008.
A. R. War, M. G. Paulraj, T. Ahmad, A. A. Buhroo, B. Hussain, S. Ignacimuthu, et al., “Mechanisms of plant defense against insect herbivores”. Plant Signal Behav, 2012; 7 (10): 1306-1320.
P. Sharma, A. B. Jha, R. S. Dubey, M. Pessarakli. “Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions”. J Bot, 2012; 2012: 1-26.
G. Adwan, M. Mhanna. “Synergistic effects of plant extracts and antibiotics on staphylococcus aureus strains isolated from clinical specimens”. J Sci Res, 2008; 3: 134-139.
E. W. Hancock. “Mechanisms of action of newer antibiotics for Gram-positive pathogens”. Lancet Infect Dis, 2005; 5: 209-218.
G. M. Shepherd. “Hypersensitivity reactions to drugs: evaluation and management”. Mt Sinai J Med, 2003, 70: 113-25.
C. T. Keith, A. A. Borisy, B. R. Stockwell. “Multicomponent therapeutics for networked systems”. Nat Rev Drug Discov, 2005, 4: 71-8.
J. G. Costa, F. F. Rodrigues, E. C. Angélico, C. K. Pereira, E. O. Sousa, G. F. Caldas, et al., “Chemical composition and evaluation antibacterial activity and toxicity of essential oil of Croton zehntneri (variedade estragol)”. Braz J Pharmacogn, 2008; 18: 583-6.
H. D. Coutinho, J. G. Costa, J. P. Siqueira-Júnior, E. O. Lima. “In vitro anti-staphylococcal activity of Hyptis martiusii Benth against methicillin-resistant Staphylococcus aureus-MRSA strains”. Braz J Pharmacogn, 2008; 8: 670-5.
American Public Health Association. “Standard methods for the examination of water and wastewater”. 19th ed, USA; APHA, 1995.
Anonymous. Coliform bacteria in drinking water supplies. https://www.health.ny.gov/environmental/water/drinking/coliform_bacteria.htm, Accessed 5 May 2016.
Anonymous. Protect yourself from coliform bacteria in well water. http://epi.publichealth.nc.gov/oee/docs/Coliform_Bacteria_WellWaterFactSt.pdf, Accessed 5 May 2016.
Anonymous. Coliform bacteria. http://extension.psu.edu/natural-resources/water/drinking-water/water-testing/pollutants/coliform-bacteria, Accessed 5 May 2016.
Anonymous. Coliform bacteria and drinking water. http://www.bfhd.wa.gov/info/coliform.php, Accessed 5 May 2016.
Anonymous. Cliff Treyens. Bacteria and private wells. http://www.nesc.wvu.edu/pdf/dw/publications/ontap/magazine/OTWI09_features/BacteriaAndPrivateWells.pdf, Accessed 5 May 2016.
Anonymous. Coliform bacteria and drinking water. http://www.doh.wa.gov/portals/1/Documents/Pubs/331-181.pdf, Accessed 5 May 2016.
D. A. Odeny, B. Jayasshree, M. Fergusow, D. Woisington, L. J. Cry, C. Gebhardt. “Development, characterization and utilization of microsatellite markers in Pigeonpea”. Plant Breed. 2007; 126: 130-6.
D. K. Abbiw. “Useful plants of Ghana, richmond intermediate technology publications and royal botanic gardens. UK; Kew, 1990.
J. A. Duke, R. Vasquez. “Amazonian ethnobotanical dictionary”. USA; CRC press: Boca Raton, 1994.
T. Amalraj, S. I. muthu. Indian J Exp Biol, 1998; 36: 1032-1033.
J. K. Grover, S. Yadav, V. J. Vats. “Medicinal plants of India with anti-diabetic potential”. J Ethnopharmacol, 2002; 81: 81-100.
B. Upadhyay, Parveen, A. K. Dhaker, A. Kumar. “Ethnomedicinal and ethnopharmaco – statistical studies of Eastern Rajasthan”. Indian J of Ethnopharmacol, 2010; 129: 64-86.
M. Luo, X. Liu, Y. Zu, Y. Fu, S. Zhang, L. Yao, et al. “Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots, induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrial pathway”. Chem Biol Interact, 2010; 188: 151-60.
D. H. Chen, H. Y. Li, H. Lin. “Studies on chemical constituents in pigeonpea leaves”. Zhong Cao Yao, 1985; 16: 134-136.
R. A. Nicholson, L. S. David, R. L. Pan, X. M. Liu. “Pinostrobin from Cajanus cajan (L.) Millsp. Inhibits sodium channel-activated depolarization of mouse brain synaptoneurosomes”. Fitoterapia, 2010; 81: 826-9.
R. S. Nanna, M. Banala, A. Pamulaparthi, A. Kurra, S. Kagithoju. “Evaluation of phytochemicals and fluorescent analysis of seed and leaf extracts of Cajanus cajan L. nt”. J Pharm Sci Rev Res, 2013; 22 (1): 11-18.
R. W. Bauer, M. D. K. Kirby, J. C. Sherris, M. Turck. “Antibiotic susceptibility testing by standard single disc diffusion method”. Ame J of Clin Path, 1966; 45: 493-496.
R. S. Hendricsen. “MIC susceptibility testing of Salmonella and Campylobacter”. A global Salmonella surveillance and laboratory support project of the World Health Organization. 2003, 1-28.
P. G. Mazzola, A. F. Jozala, L. C. de Lencastre Novaes, P. Moriel, T. C. V. Penn. “Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents”. Braz J Pharm Sci, 2009; 45 (2): 241-248.
B. W. Robert. “Diagnostic microbiology: A textbook for the isolation and identification of pathogenic microorganisms”. New York: C. V. Mosby Co., 1966.
G. L. Shore, H. D. Isenberg. “Clinical Microbiology Procedures Handbook”. 3rd ed, Washington, DC: ASM Press, 2010.
A. T. MacConkey. “Lactose-fermenting bacteria in faeces”. J Hyg, 1905; 5: 333-379.
J. F. MacFaddin. Biochemical tests for identification of medical bacteria. Philadelphia: Lipincott Williams & Wilkins, 2000.
Anonymous. Haemophilus test medium (HTM) agar. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/HaemophilusTestMedium.htm, Accessed 5 May 2016.
M. M. Cowan. “Plant products as antimicrobial agents”. Clin Microbiol Rev, 1999; 12 (4): 564-582.
D. Srinivasan, L. Perumalsamy, S. Nathan, T. Sures. “Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine”. J of Ethnopharmacol, 2001; 94: 217-222.
K. Kalimuthu, S. Vijayakumar, R. Senthilkumar. “Antimicrobial activity of the biodiesel plant, Jatropha curcas”. Intern J Pharm Bio Sci, 2010; 1: 1-5.
E. Nwachukwu, H. O. Uzoeto. “Antimicrobial activities of leaf of Vitex doniana and Cajanus cajan on some bacteria”. Researcher, 2010; 2 (3): 37-47.
S. Obiorah, E. Eze, D. Obiorah, N. Orji, C. Umedum. “Phytochemical and antimicrobial studies on the extracts from leaves of Cajanus Cajan and Eucalyptus globules”. International Conference on Environment, Chemistry and Biology, Singapore; IACSIT Press, 2012; 49: 38.
G. O. Ezeifeka, M. U. rji, T. I. Mbata, A. O. Patrick. “Antimicrobial activity of Cjanua cajan, Garcinia kola and Xylopia aethiopica on pathogenic microorganisms”. Biotechnol, 2004; 3 (1): 41-43.
P. M. Aja, E. U. Alum, N. N. Ezeani, B. U. Nwali, N. Edwin. “Comparative phytochemical composition of Cajanus cajan Leaf and Seed”. Int J Microbiol Res 2015, 6 (1): 42-46.