Volume 6, Issue 2, June 2018, Pages: 49-52
Received: Jun. 8, 2018;
Accepted: Aug. 7, 2018;
Published: Sep. 15, 2018
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Nuhu Abdullahi Mukhtar, Department of Chemistry, School of Science Education, Sa’adatu Rimi College of Education, Kumbotso, Nigeria
Maryam Nasidi Nuhu, Department of Chemistry, School of Science Education, Sa’adatu Rimi College of Education, Kumbotso, Nigeria
Jamilu Alhaji Aminu, Department of Chemistry, Kano University of Science and Technology, Wudil, Nigeria
Hadiza Abdullahi Usman, Department of Chemistry, Faculty of Sciences, Federal University Dutse, Dutse, Nigeria
Plants have been recognized long ago as rich sources of natural products for the treatment of a wide spectrum of diseases. It has been reported that plant extracts are commonly used in traditional medicine and its contribution with respect to health coverage was estimated for over 80% of the world’s population, especially in the developing world. The present investigation, qualitatively evaluated the present of some certain phytochemicals in the crude extract of the macerated plant stem obtained from three solvents (ethanol 90%, distilled water and chloroform). Ethanolic extract shows higher presence of phytochemicals over water and chloroform extract. The in-vitro antibacterial activity of crude extract of the plant’s stem was tested on one gram positive bacteria (Staphylococcus aureus) and one gram negative bacteria (Escherichia coli). The test was performed by simple Agar diffusion Assay. Zone of inhibition (mm) of the extracts was determined. Of the various extract that was tested ethanolic extract was the one which shows remarkable effect on the microbes (Escherichia Coli 9.40mm and Staphylococcus Aureus 14.60mm). Based on these discovery it might be concluded that Calligonum polygonoides possessed great potential against these human pathogens. It might also be speculated that these plant extract can be subjected to further chemical and biochemical analysis to characterize their chemical constituents and the chemical compound responsible for the antibacterial activity.
Nuhu Abdullahi Mukhtar,
Maryam Nasidi Nuhu,
Jamilu Alhaji Aminu,
Hadiza Abdullahi Usman,
Phytochemical Analysis and In-Vitro Antibacterial Activity of Chloroform, Water and Ethanolic Stem Extracts of Calliganum polygonoides (Phog), Plant.
Vol. 6, No. 2,
2018, pp. 49-52.
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
WHO 2002. Traditional medicine: Growing needs and potential. WHO Policy Perspectives on Medicines. World Health Organization, Geneva: 1–6.
Akunyili DN. The role of regulation of medicinal plants and phytomedicine in socio-economic development, AGM/SC of the Nigerian Society of Pharmacognosy. 13., 2003; 1-7.
Bhandari M. M. (1978). Flora of the Indian Desert (Scientific Publishers) Jodhpur 331-332.
Katewa SS and Galav PK (2005). Traditional herbal medicines from Shekhawati region of Rajasthan. Indian Journal of Traditional Knowledge 4 237-245.
Singh UR, Wadhwani AM and Johri BM (1996). Dictionary of Economic Plants in India. ICAR, New Delhi 37 and 60.
Kumar S, Praveen F, Goyal S and Chauhan A (2008). Indigenous herbal coolents for combating heat stress in the hot Indian Arid Zone. Indian Journal of Traditional Knowledge 7 679-682.
Mohil P (2013). Depleting diversity of Calligonum polygonoides L. with their importance in the arid region of India. Environmental Consciousness and Human Perceptions (Lambert Academic Publishing) 90-96.
Yawer MA, Ahmed E, Malik A, Ashraf M, Rasool MA and Afza N (2007). New lipoxygenase inhibiting constituents from Calligonum polygonoides. Chemistry & Biodiversity 7 1578-1585.
Bewal S, Sharma SK, Parida A, Shivam S, Rao SR and Kumar A (2009). Utilization of RAPD analyze natural genetic variation in Calligonum polygonoides L. - A Keystone species of Indian Desert. International Journal of Integrative Biology 5 148-151.
Singhi M and Joshi R (2010). Famine food of arid Rajasthan: utilization, perceptions and need to integrate social practices by bio-resolutions. EthnoMed 4 121-124.
Singh V and Pandey RR (1998). Ethenobotany of Rajasthan, India 4, 64, 80, 237 and 270.
Samejo MQ, Memon S, Bhanger MI and Khan KM (2012). Chemical composition of essential oil from Calligonum polygonoides Linn. Natural Product Research 27 619-623.
Samejo MQ, Memon S, Bhanger MI and Khan KM (2011). Preliminary phytochemical screening of Calligonum polygonoides Linn. Journal of Pharmacy Research 4 4402-4403.
Wendakoon C, Calderon P, Gagnon D 2012. Evaluation of selected medicinal plants extracted in different ethanol concentrations for antibacterial activity against human pathogens. Journal of Medicinally Active Plants 1(2).
Harborne JB, Baxter H. The handbook of natural flavonoids, Volume 1 and 2. Chichester, UK: John Wiley and Sons, 1999.
Dalal H. M. In-vitro Antibacterial Activity of Ethanol Extract of Calligonum comosum Plant Against four Human Pathogens in Saudi Arabia Department of Biology, Faculty of Science, Nora Bint Abdul-Rahman University, Postal Code 11474, Riyadh, Saudia Arabia. October 2013.
M. Q. Samejo, S. Memon, M. I. Bhanger, K. M. Khan, Nat. Prod. Res. 2013, 27, 619–623.
M. Q. Samejo, S. Memon, M. I. Bhanger, K. M. Khan, Ind. Crops Prod. 2013, 45, 293 –295.
M. Q. Samejo, S. Memon, M. I. Bhanger, K. M. Khan, J. Pharm. Res. 2013, 6, 346–349.
M. Okasaka, Y. Takaishi, K. Kogure, K. Fukuzawa, H. Shibata, T. Higuti, G. Honda, M. Ito, O. K. Kodzhimatov, O. Ashurmetov, J. Nat. Prod. 2004, 67, 1044–1046.