Selection of Salt Tolerant Somaclones for Development of Salt Stress Tolerant Varieties
Volume 3, Issue 4, July 2015, Pages: 37-46
Received: Jun. 28, 2015;
Accepted: Jul. 9, 2015;
Published: Jul. 18, 2015
Views 4522 Downloads 875
Mst. Kohinoor Begum, Bangladesh Sugarcane Research Institute, Pabna, Bangladesh
Md. Obaidul Islam, Dept. of Crop Botany, Bangladesh Agricultural University, Mymensingh, Bangladesh
The experiment were carried out at the farm of Bangladesh Sugarcane Research Institute Ishurdi, Pabna, Bangladesh during 2011-2012 cropping season. The somaclones which were produced in vitro under salinity stress evaluated in the field and after 1st year evaluation those which showed better performance were selected for this experiment to select salinity tolerant line of sugarcane. Sugarcane varieties Isd 28, Isd 35, Isd 36, Isd 37 and Isd 38 were used for selection of salinity tolerant lines. As treatments, salt were used with MS medium for in vitro plantlet regeneration. The selected somaclones were evaluated in 1st and 2nd year and after 1st year evaluation better somaclones were also evaluated under induced salinity stress conditions using different levels of salt (50 mM, 100 mM & 150 mM). Molecular characterizations were also done with the setts of selected somaclones collected from R2 generation. At the highest level of salinity (150 mM) varieties Isd 35 and Isd 37 initiated callus. Under induced salinity stress SC3 of Isd 28, SC3 of Isd 35 and SC2, SC3 of Isd 37 showed better performance in PVC pipe. Those somaclones also performed better in morpho-physiological performance at field condition in two sequential years and also produced polymorphic bands when we characterized with SSR marker. So those lines should be considered as salinity tolerant lines for further study.
Mst. Kohinoor Begum,
Md. Obaidul Islam,
Selection of Salt Tolerant Somaclones for Development of Salt Stress Tolerant Varieties, Plant.
Vol. 3, No. 4,
2015, pp. 37-46.
Aktas, L.Y.; Akca, B.T.H. and Parlak, S. 2007. Role of abscisic acid and proline treatment on induction of antioxidant enzyme activities and drought tolerance responses of Laurus nobilis L. seedlings. Fen Bilimleri Dergisi, 28:14-27.
Ashraf, M. and Foolad, M. R. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot., 59: 206-216.
Akhtar, S.; Wahid, A. and Rasul, E. 2003. Emergence, growth and nutrient composition of sugarcane sprouts under NaCl Salinity. Biol. Plantarum, 46(1): 113-116.
Bhansali, R.R. and Kishan, S. 1982. Callus and shoot formation from leaf of sugarcane in tissue culture. Phytomorphology, 32(2/3): 167-170.
Evans,D.A.; Sharp, W.R. and Medmi-Filho, H.P.1984. Somaclonal and gametoclonal variation .Amer. J. Bot., 71:759-774.
Flowers, T. J. & Yeo, A. R. 1995. Breeding for salinity resistance in crop plants: Aust. J. Plant Physiol. 22: 875-884.
Greenway, H. and Munns, R. 1980. Mechanisms of salt tolerance in non halophytes. Annual Review of Plant Physiol. 31: 149-190.
Gollek, B. 1973. Structure and function of plant cells in saline habitates-New trend in the study of salt tolerance. John Willey and Sons, New York.
Glenn, E.; Brown, J. and Khan, J. 1997. Mechanisms of salt tolerance in higher plants. In: Mechanisms of environmental stress resistance in plants. (ed.), A. Basra. p. 83-110. Harwood Acad. Pub. Amsterdam.
Hasegawa, P.M.; Bressan, R.A.; Zhu, J.K. and Bohneert, H.J. 2000. Plant cellular and molecular responses to high salinity. Annual Rev. Plant Physiol. Plant Mol. Biol., 51: 463-499.
Heinz, D.J.; Krisnamurti, M.K.; Nickell, L.G and Maretzki, A.1995. Cell, tissue and organ culture in sugarcane improvement. Plant Cell Tissue Organ cult., Narosa Pub., New Delhi.
Johari-Pireivatlou, M.; Qasimov, N. and Maralian, H. 2010. Effect of soil water stress on yield and proline content of four wheat lines. African J. Biotechnol., 9: 036–040.
Kader, M. A. 2006. Salt Stress in Rice: Adaptive Mechanisms for Cytosolic Sodium
Homeostasis. Ph.D Thesis. Dep. Plant Biol. Forest Genet. Swedish University of Agricultural Sciences .Uppsala. Sweden.
Kavi Kishor, P.B.; Sangam, S.; Amrutha, R.N.; Sri Laxmi, P.; Naidu, K.R.; Rao, K.R.S.S.; Rao, S.; Reddy, K.J.; Theriappan, P. and Sreenivasulu, N. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Curr. Sci., 88: 424-438.
Kumar, R. R.; Karajol, K. and Naik, G. R. 2011. Effect of polyethylene glycol induced water stress on physiological and biochemical responses in pigeonpea (Cajanus cajan L. Millsp.). Recent Res. Sci. Technol., 3(1): 148-152.
Liu, M.C.; Huang, Y.J. and Shih, S.C. 1972. The in vitro production of plants from several tissues of Saccharum spp. J. Agric. Ass. China, 77: 52-58.
Leroy X.J.; Leon, K. and Charles, G. 2000. Cauliflower somatic embryogenesis and analysis of regenerants stability by ISSRs. Plant Cell Rep., 19:1102-1107.
Martins, M.; Sarmento, D. and Oliveira, M.M. 2004. Genetic stability of micropropagated almond plantlets as assessed by RAPD and ISSR markers. Plant Cell Rep., 23: 492-496
Martinez-Beltran J. and Manzur C.L. 2005. Overview of salinity problems in the world and FAO strategies to address the problem. Proc. Int. Salinity Forum, Riverside, California, April 2005, p. 311–313.
Munns, R.; James, R.A. and Läuchli, A. 2006. Approaches to increasing the salt tolerance of wheat and other cereals. J. Exp. Bot., 57: 1025-1043
Pessarakli, M.1999. Handbook of Plant and Crop Stress. 2nd Edn. Revised and Expanded, Marcel Dekker, p. 1254. Inc., New York.
Palombi, M.A. and Damiano, C. 2002. Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit Actinidia deliciosa A. Chev. Plant Cell Rep., 20: 1061-1066
Saruhan, N.; Terzi, R. and Kadioglu, A. 2006. The effects of exogenous polyamines on some biochemical changes during drought stress in Ctenanthe setosa. Acta Biologica Hungarica, 57(2): 221-229.
Shahanawaz, R. M. S. 2006. DNA isolation, quantification and fingerprinting using RAPD markers of sugarcane (Saccharum officinarum L.). M. Sc. Thesis. Biotechnol. Gen. Engineer. Dept., Islamic Univ., Kushtia, Bangladesh.
Singh R.K.; Singh R.B.; Singh S.P. and Sharma M.L. 2011. Identification of sugarcane microsatellites associated to sugar content in sugarcane and transferability to other cereal genomes. Euphytica, 182: 335-354
SRDI, 2003. Soil salinity in Bangladesh 2000. Soil Resource Development Institute Watanabe, S.; Kojima, K.; Ide, Y. and Satohiko, S. 2000. Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tissue Organ Cult., 63(3): 199-206.
Zhu, J. K., 2001. Plant salt tolerance. Trends Plant Sci., 6, 66–71.