Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes
Volume 3, Issue 1, January 2015, Pages: 1-7
Received: Aug. 21, 2014; Accepted: Sep. 9, 2014; Published: Dec. 15, 2014
Views 2808      Downloads 278
Esra Maltas, Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey
Aslı Dageri, Department of Plant Protection, Faculty of Agriculture, Ankara U
Hasibe Cingilli Vural, Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey
Salih Yildiz, Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey
Article Tools
Follow on us
Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.
Echinacea Purpurea and Echinacea Pallida, Molecular Marker, PCR, nDNA Antioxidant Activity, Fatty acid, Flavonoid
To cite this article
Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz, Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes, Plant. Vol. 3, No. 1, 2015, pp. 1-7. doi: 10.11648/j.plant.20150301.11
Amarowicz R, Naczk M and Shahidi F (2000) Antioxidant Activity of Crude Tannins of Canola and Rapeseed Hulls. JAOCS 77: 957-961.
Baum BR, Mechanda S, Livesey JF, Binns SE, Arnason JT (2001) Predicting quantitative phytochemical markers in single Echinacea plants or clones from their DNA fingerprints. Phytochem. 56 (6): 543-549.
Briskin DB (2000). Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant Physiol. 124:507–514.
Chen ZY, Chan PT, Kwan KY and Zhang A (1997) A reassessment of the antioxidant activity of conjugated linoleic acid. J. Am. Oil Chem. Soc. 73: 749–753.
Cruz VM, Luhman VR, Marek LF, Rife CL, Shoemaker RC, Brummer EC and Gardner CAC (2007) Characterization of flowering time and SSR marker analysis of spring and winter type Brassica napus L. germplasm. Euphytica 153: 43-57.
Dellaporta SL, Wood J and Hicks JB (1983) A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1: 19-21.
Diers BW and Osborn TC (1994) Genetic diversity of oilseed Brassica napus germplasm based on restriction fragment lenght polymorphism. Theor. Appl. Gen. 88: 662-668.
Fagali N and Catalá A (2008) Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH• techniques. Biophysical Chem. 137: 56–62.
Goel V, Chang C, Slama JV, Barton R, Bauer R, Gahler R, Basu TK (2002) Alkylamides of Echinacea purpurea stimulate alveolar macrophage function in normal rats. International Immunopharmacology, 2 (2-3:)381-387.
Haldén C Nilsson NO, Rading I, Säll T (1994) Evaluation of RFLP and RAPD markers in a comparison of Brassica napus breeding lines. Theoretical and Applied Genetics 88: 123-128.
Huang DJ, Lin CD, Chen HJ, Lin YH (2004) Antioxidant and antiproliferative activities of sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) constituents. Botanical Bulletin of Academia Sinica, 45: 179–186.
Jaccard P (1908) Novelles recgerches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat. 44: 223-270.
Jamison JR (2003) Echinacea. Clinical Guide to Nutrition & Dietary Supplements in Disease Management, 501-505.
Joshi K, Chavan P, Warudee D, Patwardhan B. (2004). Molecular markers in herbal drug technology. Current Science 87: 157–165.
Kapteyn J, Goldsbrough PB and Simon JE (2002) Genetic relationships and diversity of commercially relevant Echinacea species. Theor. Appl. Genet. Berlin; Springer-Verlag. Aug 2002. v. 105 (2/3) p. 369-376.
Kresovich S, Williams JGK, McFerson JR, Routman EJ, Schaal BA (1992) Characterization of genetic identities and relationships of Brassica oleracea L. via random amplified polymorphic DNA assay. Theoretical and Applied Genetics 85: 190-196.
Lie-Zhao L, Jin-Ling M, Na L, Li C, Zhang-Lin T, Xue-Kun Z, Jia-Na LI (2006) QTL Mapping of Seed Coat Color for Yellow Seeded Brassica Napus. Actu GeneticaSinica, 33: 181-187.
Liu X, Dong M, Chen X, Jiang M, Lv X and Yan BG (2007) Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba. Food Chem. 105 (2) 548-554.
MacDonald HB (2000) Conjugated linoleic acid and disease prevention: a review of current knowledge, J. Am. Coll. Nutr. 19: 111–118.
Naczk M, Amarowicz R, Sullivan A and Shahidi F (1998) Current Research Developments on Polyphenols of Rapeseed/Canola: A Review, Food Chem. 62: 489–502.
Pellati F, Benvenuti S, Magro L, Melegari M, Soragni F (2004) Analysis of phenolic compounds and radical scavenging activity of Echinacea spp. Journal of Pharmaceutical and Biomedical Analysis 35(2): 289-301
Sambrook J, Fritsch EF, Manniatis T (1989). Molecular Coloning: A Laboratory Manual, 15 section, p. 18.47-18.76. Cold. Spring Harbor, New York.
Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric. 76: 270-276.
Shengwu H, Ovesná JK, Kučera V and Vyvadilová M (2003) Evaluation of genetic diversity of Brassica napus germplasm from China and Europe assessed by RAPD markers. Plant Soil Environ. 49 (3): 106-113.
Stanisavljević I, Stojičević S, Veličković D, Veljković V, Lazić M (2009) Antioxidant and Antimicrobial Activities of Echinacea (Echinacea purpurea L.) Extracts Obtained by Classical and Ultrasound Extraction. Chinese Journal of Chemical Engineering, 17(3): 478-483.
Techen N, Crockett SL, Khan IA, Scheffler BE (2004) Authentication of Medicinal Plants Using Molecular Biology Techniques to Compliment Conventional Methods. Curr. Med. Chem., 11, 1391-1401.
Toselli F, Matthias A, Gillam EMJ (2009) Echinacea metabolism and drug interactions: The case for standardization of a complementary medicine. Life Sciences, 85( 3-4): 97-106.
Yurawecz MP, Hood JK, Mossoba MM. Roach JA, Ku Y (1995) Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid. Lipids 30: 595–598.
Welsh J, Peterson C, Clelland MMc (1991) Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucleic Acids Res. 19: 303-306.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA and Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic-markers. Nucleic Acids Res. 18: 6531-6535.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186