Efficiency of Nano-formulations of Neem and Peppermint Oils on the Bionomics and Enzymatic Activities of Agrotis ipsilon Larvae (Lepidoptera: Noctuidae)
International Journal of Natural Resource Ecology and Management
Volume 4, Issue 5, September 2019, Pages: 102-111
Received: Jun. 24, 2019; Accepted: Jul. 19, 2019; Published: Aug. 12, 2019
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Abdel Rahman Hussein Amin, Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Alaa El Din Bayoumi, Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Nadia Zikry Dimetry, Department of Pests and Plant Protection, National Research Centre, Dokki, Cairo, Egypt
Dalia Abdellah Youssef, Department of Pests and Plant Protection, National Research Centre, Dokki, Cairo, Egypt
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Nanotechnology is considered to be a development way to gain efficient and potential approaches towards pest control. The effect of both neem and peppermint oils as bulk, nano and loaded nano-emulsions have been studied against the different biological aspects of the 2nd larval instar of Agrotis ipsilon (Hufn.) under laboratory conditions. Their effects on enzymatic activities were investigated for the 2nd and 4th larval instars. The effects of adding the different formulations of either neem or peppermint oils to artificial diet of 2nd instar larvae show significant elongation of the larval duration; percentage mortalities were increased as well as larval malformations. It was recorded that nano-formulations (emulsion or loaded) significantly decreased the pupal weight and significantly increased the pupal duration, percentage pupal mortalities and pupal malformation. Adult longevity show insignificant effect, while female fecundity and percentage egg fertility show significant decrease in comparison with the control. Results of enzymatic activities show marked effects of the three formulations of either neem or peppermint oil. Significant inhibitions were recorded for amylase, invertase, trehalase, protease and alkaline phosphatase. However, significant increase in the activities of cuticle phenoloxidase and chitinase were recorded. Marked variations were recorded between nano-formulations and the bulk form.
Neem Oil, Peppermint Oil, Nano-formulations, BIONOMICS, Enzymes, Agrotis ipsilon
To cite this article
Abdel Rahman Hussein Amin, Alaa El Din Bayoumi, Nadia Zikry Dimetry, Dalia Abdellah Youssef, Efficiency of Nano-formulations of Neem and Peppermint Oils on the Bionomics and Enzymatic Activities of Agrotis ipsilon Larvae (Lepidoptera: Noctuidae), International Journal of Natural Resource Ecology and Management. Vol. 4, No. 5, 2019, pp. 102-111. doi: 10.11648/j.ijnrem.20190405.11
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Muhammad, S.; U. Farman; N. Muhammad; A. Muhammad; A. U. R. Saljoqi and Z. Muhammad (2007) Effect of various insecticides for the control of Potato cutworm (Agrotis ipsilon Huf., Noctuidae: lepidoptera) at kalam swat. Sahad J. Agric., 23 (2): 423-426.
Watson, R. N. (2016) Control of greasy cutworm (Lepidoptera: Noctuidae) in seedling maize. New Zealand J. Exp. Agric., 9 (3-4): 351-356.
Busching, M. K. and F. T. Turpin (1977) Survival and development of black cutworm (Agrotis ipsilon) larvae on various species of crop plants and weeds. Environ. Entomol, 6: 63-65.
EI-Salamouny S., Lange M., Jutzi M., Huber J., Jehle J. A. (2003) Comparative study on the susceptibility of cutworms (Lepidoptera: Noctuidae) to Agrotis segetum nucleopolyhedrovirus and Agrotis ipsilon nucleo polyhedrovirus. J. Invert. Pathol. 84: 75–82. doi: 10.1016/j.jip.2003.08.005.
Amoabeng, B. W.; G. M. Gurr; C. W. Gitau; H. I. Nicol; L. Munyakazi and P. C. Stevenson (2013) Tri-Trophic Insecticidal Effects of African Plants against Cabbage Pests. Plos one, 8 (10): 1371-1382.
Dimetry, N. Z. (2012). Prospects of botanical pesticides for the future in integrated pest management program (IPM) with special reference to neem uses in Egypt. Arch. Phytopathol. Plant Protec., 45: 1138-1161.
Dimetry, N. Z.; A. Y. El-Laithy; A. M. E. Abd El-salam and A. E. El-Saiedy (2013) Management of the major piercing sucking pests infesting cucumber under plastic house conditions. Arch. Phytopathol. Plant Protec.; 46: 158-171.
Mubayi, A.; S. Chatterji; P. M. Rai and G. Watal (2012) Evidence based green synthesis of nanoparticles. Adv. Materials Methods., 3 (6): 519-525.
Raveendran, P.; J. Fu and S. L. Wallen (2006) A simple and ‘‘green’’ method for the synthesis of Au, Ag, and Au-Ag alloy nanoparticles. Green Chem., 8: 34-38.
Ghormade, V.; V. D. Mukund and M. P. Kishore (2011) Perspectives for nano-biotechnology enabled protection and nutrition of plants. Biotech. Adv., 29: 792–803.
Anjali, C. H.; S. S. Khan; K. Margulis-Goshen; S. Magdassi; A. Mukherjee and N. Chandrasekaran (2010) Formulation of water-dispersible nanopermethrin for larvicidal applications. Ecotox. Environ. Safe., 73: 1932–1936.
Anjali, C. H.; Y. Sharma; A. Mukherjee and N. Chandrasekaran (2012) Neem oil (Azadirachta indica) nanoemulsion a patent larvicidal agent against Culex quinquefasciatus. Pest. Manag. Sci, 68 (2): 158–63.
Dimetry, Nadia Z.; Amin, A. H.; Bayoumi, A. E.. Abdel- Raheem, M. A. and Youssef, Dalia A. (2019) Comparative Toxicity between Neem and Pepper Mint Oils Nano Formulations against Agrotis ipsilon (Hufn.) larvae (Lepidoptera: Noctuidae). J. Botanic. Res., 01, Issue 01, April 2019: 13-19.
Frederiksen, H. K.; H. G. Kristensen and M. Pedersen (2003) Solid lipid microparticle formulations of the pyrethroid gamma-cyhalothrin-incompatibility of the lipid and the pyrethroid and biological properties of the formulations. J. Control. Release., 86: 243–252.
Guenther, E. (1961) The essential oils. Vol (4) Von Nostan and con. N. York. pp. 132-147.
Azhari Siddeeg, Zakaria A. Salih, Rabab M. E. Mukhtar & Ali O. Ali (2018) Extraction and Characterizati 1on of Peppermint (Mentha piperita) Essential Oil and its Assessment as Antioxidant and Antibacterial. Gezira Journal of Engineering and Applied Sciences vol 13 (1): 1-15, 2018.
Yousef, Dalia A.; Bayoum, A. E.; Dimetry, N. Z.; Amin, A. H. and Hoballah, E. M. (2018) Evaluating Effect of Peppermint Oil (Mentha pipreta) and its Nano-Formulations on Some Enzmatic Activities and Bionomics of Cotton Leaf Worm Spodoptera littoralis.
Lertsutthiwong, P.; K. Noomun; N. Jongaroonngamsang; P. Rojsitthisak U. and Nimmannit (2008) Preparation of alginate nanocapsules containing turmeric oil. Carbohydr. Polym., 74: 209–214.
Ishaaya, I. and Swirski, E. (1976) Trehalase, invertase and amylase activities in the black scale Saissetia oleae, and their relation to host adaptability. J. Insect Physiol., 22: 1025-1029.
Ascher, K. R. and Ishaaya, S. (2004). Antifeedant and protease and amylase inhibiting activity of fentin acetate in Spodoptera littoralis larvae. Pest. Biochem. Physiol., 3 (3): 326-336.
Ishaaya, I.; I. Moore and I. Joseph (1971) Protease and amylase activity in larvae on the Egyptian cotton worm, Spodoptera littoralis. J. Insect Physiol., 17: 45-95.
Powell, M. E. A. and M. J. H. Smith (1954) The determination of serum acid and alkaline phosphatases activity with 4-amino antipyrine. J. Clin. Pathol., 7: 245- 248.
Ishaaya, I. and J. E. Casida (1974) Dietary TH 6040 alters composition and enzyme activity of housefly larval cuticle. Pestic. Biochem. Physiol., 4: 484-490.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitative of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
Rao, B. R.; P. Rajasekhar; M. Venkataiah and N. V. Rao (1995) Bio-efficacy of ‘Neem Azal’ (azadirachtin 10,000 ppm) against cotton bollworm, Helicoverpa armigera (Hübner). J. Entomol. Res., 19 (4): 329-333.
Ma, X.; Lee, J. G.; Y. Dengand A. Kolmakov (2010) Interactions between engineered nanoparticles (ENPs) and plants: Phytotoxicity, uptake and accumulation. Sci. of the Total Environ., 408: 3053–3061.
Pineda, S.; A. M. M1artinez; J. I. Ficueroa; M. I. Schneider; P. D. Estal; E. Vinuela; B. Gomez; G. Smagghe and F. Budia (2009) Influence of azadirachtin and methyl oxyfenozide on life parameters of S. littoralis (Lepidoptera: Noctuidae). J. Econ. Entomol., 102 (4): 1490-1496.
Mohamed, Samar, S. I. (2016) Applications of some biotechnological methods for controlling the most important pests of potatoes. Ph.D. Thesis. Fac. Science for Girls. Al-Azhar University. 306 pp.
Sharaby, A. and A. El-Nojiban (2015) Evaluation of some plant essential oils against the black cutworm Agrotis ipsilon. Global J. Adv. Res., 2 (4): 701-711.
Huang, Z.; P. Shi; J. Dai and J. Du (2004) Protein metabolism in Spodoptera litura (F.) is influenced by the botanical insecticides azadirachtin. Pest biochem. Physiol., 80 (2): 85-93.
Isman, M. B.; S. Miresmailli and C. Machial (2011) Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumers products. Phytochem. Rev., 10: 197-204.
Gutierrez, L.; A. Escudero; R. Battle and C. Nerin (2009) The effect of mixed antimicrobial agents and flavors in active packaging films. J. Agric. Food Chem., 57: 8564-8571.
Rao, P. J.; K. H. Kumar; S. Sing and B. Sibrhmanyam (1999) Effect of Artemisia annua oil on development and reproduction of Dysdecus koenigii F. Division Ent. India. J. Appl. Ent., 123: 315-318.
Rharrabe, K.; N. Bouayad and F. Saah (2009) Effect of ingested 20-hydroxycdysone on development and midgut epithelial cells of Plodia interpunctella (Lepidoptera, Pyralidae). Pestic. Biochem. Physiol., 93: 112-119.
Hill, S. R. and I. Orchard (2005) In vitro analysis of the digestive enzymes amylase and α-glucosidase in the midgets of Locusta migratoria L. in response to the myosppressin, J. Insect Physio., 51: 1-9.
Etebari, K.; S. Z. Mirhoseini and L. Matindoost (2005) A study on intraspecific biodiversity of eight groups of silk.; Mirhoseini worm (Bombyx mori) by biochemical markers. Insect. Sci., 12: 87-94.
Farrag, A. A.; T. A. Abd-Elfattah; G. M. Abdelatef and M. K. El–Dydamony (2015) Effect of four bioactive compounds separately and in combination with enzymes of Schistocerca gregaria. Plant Prot. J. Path., 6: 871–883.
Santoyo, I. G. and Aguilar, A. C. (2012) Phenol oxidase: a key component of the insect immune system. Entomol. Exp. Applicata, 142 (1): 1–16.
Gilmour, D. (1961) The biochemistry of insects. Academic press, New York and London.
Senthil-Nathan, Sengottayan (2013) Physiological and biochemical effect of neem and other Meliaceae plants secondary metabolites against Lepidopteran insects. Front. Physiol. 4: 359, 20 December 2013. https: //doi.org/10.3389/fphys.2013.co359.
Osman, H. H.; H. F. Abdel-Hafez and A. A. Khidr (2015) Comparison between the Efficacy of Two Nano-Particles and Effective Microorganisms on Some Biological and Biochemical Aspects of Spodoptera littoralis. J. Agr. Innov. Res., 3 (6): 2319-1473.
Retnakaran, A. and G. G. Grant (1985) Control of the oak-leaf shredder, Croesia semi purpurana (Kearfott) (Lepidoptera: Tortricidae), by aerial application of diflubenzuron. Can. J. Ent., 117: 363-369.
Liburd, E. O.; J. E. Funderburk and S. M. Olson (2000) Effect of biological and chemical insecticides on Spodoptera species (Lep. Noctuidae) and marketable yield of tomatoes. J. App. Entomol., 124: 19-25.
Abdel-Hakim, E.; N. Y. Salem; E. Sammour and S. S. Ibrahim (2011) Influence of three plant extracts on some biological and biochemical aspects of the black cutworm Agrotis ipsilon (Hufn.) Bull. NRC, 36 (3): 223-237.
41-Abd El-Aziz, M. F. (2011) Bioactivities and biochemical effects of marjoram essential oil used against potato tuber moth Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae). Life Science J., 8 (1): 288-297.
Shoukry, I. F.; A. F. A. Khalaf; A. Fattah and and K. S. Khater (2003) Toxicological evaluation of some botanical oils on biochemical aspects in the Indian meal moth Plodia interpunctella (Hb.) (Lepidoptera: Pyralidae). Egypt. J. Biol., 5: 155-163.
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