Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia
International Journal of Science, Technology and Society
Volume 5, Issue 4, July 2017, Pages: 62-66
Received: Feb. 28, 2017;
Accepted: Apr. 19, 2017;
Published: Jun. 3, 2017
Views 2271 Downloads 157
Noha Ali Ibrahim, Chemistry Department, Omdurman Islamic University, Khartoum, Sudan
Safa Khalid Musa, Chemistry Department, Omdurman Islamic University, Khartoum, Sudan
Shima Mohammed Yassin, Chemistry Department, Omdurman Islamic University, Khartoum, Sudan
Nosiba Hashim Abuniama, Chemistry Department, Omdurman Islamic University, Khartoum, Sudan
Sufyan Awadalkareem, Chemistry Department, Omdurman Islamic University, Khartoum, Sudan; Medical Biochemistry Research Department, Medicinal and Aromatic Plants Research Institute, National Center for Research, Khartoum, Sudan
Alsiddig Osama, Chemistry Department, Omdurman Islamic University, Khartoum, Sudan
Mohamed N. Abdalaziz, Medical Biochemistry Research Department, Medicinal and Aromatic Plants Research Institute, National Center for Research, Khartoum, Sudan; Department of Chemistry, Faculty of Pure and Applied Science, International University of Africa. Khartoum, Sudan
Follow on us
The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.
in-vitro, FT-IR, Gas Chromatography–Mass Spectrometry (GC-MS), Antimicrobial Activity, Essential Oils, Belpharis linariifolia (Seeds)
To cite this article
Noha Ali Ibrahim,
Safa Khalid Musa,
Shima Mohammed Yassin,
Nosiba Hashim Abuniama,
Mohamed N. Abdalaziz,
Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia, International Journal of Science, Technology and Society.
Vol. 5, No. 4,
2017, pp. 62-66.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Srivastava J, Lambert J and Vietmeyer N. Medicinal plants:An expanding role in development. World Bank Technical, 1996, 320.
Parekh J and Chanda S. In vitro antimicrobial activity of Trapa natans L. fruit rind extracted in different solvents. African Journal of Biotechnology, 6 (6), 2007, 760-770.
Rinoldi MG. Problems in the diagnosis of invasive fungaldiseases. Review of infectious diseases, 13, 1991, 493-495.
Meng JH, Zhao SH, Doyle MP, Joseph SW. Antibioticresistance of Escherichia coli O157: H7 and O157: NM isolatedfrom animals, food and humans. Journal of Food Protection, 61, 1998, 1511-1514.
Perreten V, Giampa N, Schuler-Schmid U, Teuber M. Antibiotic resistance genes in coagulase-negativestaphylococci isolated from food. Systematic and AppliedMicrobiology, 21, 1998, 113-120.
Kallio, H.; Kerrola, K.; Alhonmaki, P. Carvone and limonene in caraway fruits (Carum carvi L.) analyzed by supercritical carbon dioxide extraction-gas chromatography. J. Agric. Food Chem.1994, 42, 2478-2485.
Burkil. H. M. The Useful Plants of West Tropical Africa. Blepharis linariifolia. Royal Botanic Garden; Kew. (1985-2004). [updated 2016 March 02; cited 2016 February 12] Available from: http://tropical.theferns.info/viewtropical.php?id=Blepharis+linariifolia.
Wamtinga Richard Sawadogo, Aline Meda, Charles Euloge Lamien and Martin Kiendrebeogo. Phenolic Content and Antioxidant Activity of Six Acanthaceae from Burkina Faso. Journal of Biological Sciences. 2006; 6(2): 249-252.
National Committee for Clinical Laboratory Standards (NCCLS) (1999). Performance standards for antimicrobial susceptibility testing; ninth informational supplement. Wayne, Pensilvania document M100-S9, Vol. 19. No. 1.
Silva Junior AA, Vizotto VJ, Giongi E, Macedo SG, Marques LF (1994). Plantas medicinais, caracterização e cultivo. EPAGRI. Bol. Técnico Florianópolis, pp. 68: 1-71.