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Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology
Science Discovery
Volume 5, Issue 5, October 2017, Pages: 307-311
Received: Jul. 19, 2017; Published: Jul. 19, 2017
Views 1403      Downloads 100
Authors
Liu Yan, College of Biological Foods, Changchun University of Science and Technology, Changchun, China
Dai Xujie, College of Biological Foods, Changchun University of Science and Technology, Changchun, China
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Abstract
Kombucha cellulose is a kind of secondary metabolites produced by microorganisms. It is a kind of extracellular polysaccharide which is linked with β-1,4 glycosides. Compared with lignocellulose, it is the key focus of the current research in society, with a complex three dimensional reticular structure,material-combining ability, good biocompatibility, high purity, outstanding water-holding capacity, whichcan play an important role in many areas. Optimization of culture medium by response surface method to determine the optimum Kombucha culture conditions. The optimum culture condition was: Black Tea 2.16g, sucrose 27.33g, bran 4.35g, inoculum 23.03%,culture 48h, under these conditions, the growth of biofilm thickness of Kombucha can reach 0.76cm. At the same time, the growth rate and the establishment of Kombucha Black Tea between sucrose, wheat bran and the inoculation amount of two polynomial model, the model can predict the growth status of Kombucha, can obtain the optimal conditions.
Keywords
Kombucha Cellulose, Optimization, Culture Conditions, Response Surface
To cite this article
Liu Yan, Dai Xujie, Optimization of the Culture Conditions of Kombucha Cellulose by Response Surface Methodology, Science Discovery. Vol. 5, No. 5, 2017, pp. 307-311. doi: 10.11648/j.sd.20170505.11
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