The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis
Science Discovery
Volume 4, Issue 3, June 2016, Pages: 161-164
Received: Jun. 1, 2016; Published: Jul. 5, 2016
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Authors
Liu Pangyuan, National Engineering Research Center for Vegetables, Beijing, China
Song Shuhui, National Engineering Research Center for Vegetables, Beijing, China
Zhao Hong, National Engineering Research Center for Vegetables, Beijing, China
He Weiming, National Engineering Research Center for Vegetables, Beijing, China
Wang Wenqi, National Engineering Research Center for Vegetables, Beijing, China
Wang Huijie, National Engineering Research Center for Vegetables, Beijing, China
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Abstract
Based on the limited understanding of the former researchers on RAPD marker, numbers of cultivars of purple yam were utilized to evaluate the application of RAPD in cultivar identification of purple yam. The results showed that RAPD was a stable DNA marker technique, and it could be scientifically and easily used in cultivar identification purple yam. The RAPD fingerprints of purple yam crops, which were derived from primers with different number of nucleotides length, were different in band number, polymorphism and band clarity. Basically, RAPD primers with S1255 can show DNA fingerprint clearly in purple yam for the generation, while RAPD primers with S1255 is more suitable purple yam crops.
Keywords
Purple Yam, RAPD, Cultivar Identification
To cite this article
Liu Pangyuan, Song Shuhui, Zhao Hong, He Weiming, Wang Wenqi, Wang Huijie, The Molecular Identification Method for Purple Yam Cultivar Based on Genome RAPD Analysis, Science Discovery. Vol. 4, No. 3, 2016, pp. 161-164. doi: 10.11648/j.sd.20160403.11
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