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Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger
Science Discovery
Volume 4, Issue 2, April 2016, Pages: 70-74
Received: May 3, 2016; Published: May 4, 2016
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Authors
Zheng Lili, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China; Hainan Key Laboratory of Banana Genetic Improvement, Haikou, China
Sheng Zhanwu, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China; Hainan Key Laboratory of Banana Genetic Improvement, Haikou, China
Ai Binling, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China; Hainan Key Laboratory of Banana Genetic Improvement, Haikou, China
Zheng Xiaoyan, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China; Hainan Key Laboratory of Banana Genetic Improvement, Haikou, China
Jin Zhiqiang, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China; Hainan Key Laboratory of Banana Genetic Improvement, Haikou, China
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Abstract
Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtainedmutant strain of Xylanase production high ability. The results indicate that: the mutagenesis of UV irradiation and fast neutron irradiation was sensitive to increasing Xylanase activity. UV irradiation seted at 256 nm wavelength,the vertical distance was 20cm,exposure time was 90s, obtained mutant UV256-90 of the emzyme activity was 170.79U/ml,it was 2.18 times of the original strain.The rate 0.0025Gy / s of fast neutron irradiation and vertical distance 12cm,the optimal dose is 0.75Gy, screened out the mutant Fn5-2, the activity is 134.77U/ml,its 1.72 times of the original strain. Mutagenesis by diethyl sulfate, sodium nitrite, and nitrosoguanidine, enzyme activityof the optimal mutant strains were recently starting strain of 1.51 times, 1.46 times, and 1.38 times.Two physical mutagenic methods include ultraviolet mutagenesis and fast neutron irradiation were more sensitive on producing xylanase based on aspergillus Niger.
Keywords
Mutation, AspergillusNiger, Xylanase,EnzymeActivity
To cite this article
Zheng Lili, Sheng Zhanwu, Ai Binling, Zheng Xiaoyan, Jin Zhiqiang, Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger, Science Discovery. Vol. 4, No. 2, 2016, pp. 70-74. doi: 10.11648/j.sd.20160402.13
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