Saccharification of Ulva Lactuca Via Pseudoalteromonas Piscicida for Biofuel Production
Journal of Energy and Natural Resources
Volume 3, Issue 6, December 2014, Pages: 77-84
Received: Sep. 22, 2014; Accepted: Oct. 10, 2014; Published: Nov. 24, 2014
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Authors
El-Naggar M. M., Microbiology Lab., Environ. Div., National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Abdul-Raouf U. M., Botany and Microbiology Department, Faculty of Science, Al-Azhar University-Assuit Branch. Egypt
Ibrahim H. A. H., Microbiology Lab., Environ. Div., National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
El-Sayed W. M. M., Microbiology Lab., Environ. Div., National Institute of Oceanography and Fisheries (NIOF), Hurghada, Egypt
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Abstract
Pseudoalteromonas piscicida WM21 was isolated from seawater at Hurghada, Red Sea, Egypt. It was promising to hydrolyze the polysaccharides of Ulva lactuca. Ulva lactuca contained 44% carbohydrates, 5% lipids, 16% proteins, 12% Fibers and 23% ash. Optimization of reducing sugars production by P. piscicida WM21 was investigated using Plackett- Burmman design. The main effect data as well as the t-test results suggested that the beef extract and inoculum size are the most effective variables that controlled the reducing sugar produced by P. piscicida. Considerable positive effects of the high levels of substrate concentration and low levels of incubation period were also suggested. On the other hand, variations within the examined levels of pH levels, NaCl and peptone recorded slight effects. While the main effect data as well as the t-test results suggested that the substrate concentration and incubation period were the most effective variables that controlled amylase activity produced by P. piscicida. To evaluate the accuracy of the applied Plackett-Burman statistical design, a verification experiment was carried out. The predicted near optimum and far from optimum levels of the independent variables were examined and compared to the basal condition settings. The applied near optimum condition, resulted in approximately 56 mg/g increase in reducing sugar with 6 mm amylase activity by P. piscicida when compared to the basal medium formulation, while the conditions predicted to be far from optimal recorded approximately 45 mg/g decreases in reducing sugar with 3 mm amylase activity. These results supported the predictions of the applied Plackett-Burman experiment for enhancement of reducing sugar production by marine microorganisms.
Keywords
Reducing sugar, Ulva lactuca, Pseudoalteromonas piscicida, Saccharification process, Biofuel
To cite this article
El-Naggar M. M., Abdul-Raouf U. M., Ibrahim H. A. H., El-Sayed W. M. M., Saccharification of Ulva Lactuca Via Pseudoalteromonas Piscicida for Biofuel Production, Journal of Energy and Natural Resources. Vol. 3, No. 6, 2014, pp. 77-84. doi: 10.11648/j.jenr.20140306.11
References
[1]
AOAC (Association of Analytical Chemists), (2000). Official Methods of Analysis of Association of Analytical Chemist. Horwitz, W., Gaithersburg, Maryland, USA.
[2]
Austin, B. (1988). The marine environment. In: Marine Microbiology, (pp.1-11). Cambridge: Cambridge University Press.
[3]
Begum, M.F. and Alimon, A.R. (2011). Assessment of some wild Aspergillus species for cellulase production and characterization. African Journal of Microbiology Research, 5(27), 4739-4747.
[4]
Borowitzka, M. (1992). Algal biotechnology products and processes – matching science and economics. J. Appl. Phycol; 4, 267–79.
[5]
Box, G.E.P., and Draper, N. R. (1987). Empirical Model Building and Response Surfaces, John Wiley & Sons, New York, NY.
[6]
Carolissen-Mackacy, V., Arendse, G. and Hastings, J.W. (1997). Purification of bacteriocins of lactic acid bacteria: problems and proteins. Food Microbiol; 34, 1-16.
[7]
Chynoweth, D.P. (2002). Review of Biomethane from Marine Biomass. Department of Agricultural and Biological Engineering, University of Florida, Gainesville, Florida, USA.
[8]
El-Helow, E.R. and El-Ahawany, A.M. (1999). Lichenase production by catabolite repression resistant Bacillus subtilis mutants: Optimization and formation of an agro-industrial by product medium. Enz. Microbiol. Technol; 24, 325-331.
[9]
Fritze, D., Flossdorf J. and Claus, D. (1990). Taxonomy of alkaliphilic Bacillus strains. International Journal of Systematic Bacteriology, 40, 92-97.
[10]
Ganzon-Fortes, E.T. (1991). Characteristics and economic importance of seaweeds. In: Proceedings of the seaweed research training and workshop for project leaders. Philippine Council for Aquatic and Marine Research and Development.
[11]
Ge, L., Wang, P. and Mou, H. (2011). Study on saccharification techniques of seaweed wastes for the transformation of ethanol. Renewable Energy, 36, 84-89.
[12]
Hong, L.S., Ibrahim, D. and Omar I.C. (2013). Effect of physical parameters on second generation bio-ethanol production from oil palm frond by Saccharomyces cerevisiae. BioResource, 8(1), 969-980.
[13]
Horikoshi, K. (1999). Introduction: Definition of Alkaliphilic Organisms. In Alkaliphiles. (Kodansha Ltd., Tokyo, 1999b), p.1.
[14]
Hu, Z., Lin, B.K., Xu, Y., Zhong, M.Q. and Liu, G.M. (2009) Production and purification of agarase from a marine agarolytic bacterium Agarivorans sp. HZ105. Journal of Applied Microbiology, 181–190.
[15]
John, R.P., Anisha, G.S., Nampoothiri, K.M. and Pandey, A., (2011). Micro and macroalgal biomass: a renewable source for bioethanol. Bioresour Technol; 102, 186–93.
[16]
Koppram, R., Nielsen, F., Albers, E., Lambert, A., Wännström, S., Welin, L., Zacchi, G. and Olsson, L. (2013). Simultaneous saccharification and co-fermentation for bioethanol production using corncobs at lab, PDU and demo scales. Biotechnology for Biofuels, 6, 2-10.
[17]
Lau, S.C.K., Tsoi, M.M.Y., Li, X., Dobretsov, S., Plakhotnikova, Y. Wong, P-K. and Qian, P-Y. (2005). Pseudoalteromonas spongiae sp. nov., a novel member of the c-Proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters. International Journal of Systematic and Evolutionary Microbiology, 55, 1593–1596.
[18]
Malek, , N.A., , Q.M. and N. (1988). Bacterial cellulases and saccharification of lignocellulosic materials. Enzyme and Microbial Technology, 2, 750–753.
[19]
Margesin, R., Gander, S., Zacke, G., Gounot, A.M. and Schinner, F. (2003). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles, 7, 451–458.
[20]
Matsumoto, M., Yokouchi, H., Suzuki, N., Ohata, H. and Matsunaga, T. (2003). Saccharification of Marine Microalgae Using Marine Bacteria for Ethanol Production. Applied Biochemistry and Biotechnology, 4(6): 105–108.
[21]
Miller, G.L., (1959). Use of dinitrosalicylic acid for determination of reducing sugar. Analytical Chemistry, 31, 426–428.
[22]
Monaghan, R.L. and Koupal, L.R. (1989). Use of the Placket and Burman technique in a discovery program for new natural products. Novel Microbes: Products for Medicine and Agriculture, Chapter 2, 25-32.
[23]
Myers, R.H. and Montgomery, D.C. (1995). Response surface methodology: process and product optimization using designed experiments. John Wiley and Sons Inc., New York, N.Y.
[24]
Nelson, E.J. and Ghiorse, W.C. (1999). Isolation and identification of Pseudoalteromonas piscicida strain Cura-d associated with diseased damselfish (Pomacentridae) eggs. Journal of Fish Diseases, 22, 253-260.
[25]
Ooijkaas, L.P., Wilkinson, E.C., Tramper, J. and Buitelaar, R.M., (1998). Medium optimization for spore production of Conithyrium minitans using statistically-Based experimental designs. Biotechlnol. Bioeng. 64 (1), 92-100.
[26]
Pádua, M., Fontoura, P.S.G. and Mathias, A.L. (2004). Chemical Composition of Ulvaria oxysperma (Kützing) Bliding, Ulva lactuca (Linnaeus) and Ulva fascita (Delile). 47(1), 49-55.
[27]
Park, J.I., Woo, H.C. and Lee, J.H. (2008). Production of bio-energy from marine algae: status and perspectives. Korean Chem. Eng. Res; 46 (5), 833–844.
[28]
Philippidis, G..P, Smith, T.K., and Wyman, C.E. (1993). "Study of the Enzymatic Hydrolysis of Cellulose for Production of Fuel Ethanol by the Simultaneous Saccharification and Fermentation Process." Biotechnology and Bioengineering, 41, 846-853.
[29]
Plackett, R.L. and Burman, J.P. (1946). The design of optimum multifactorial experiments. Biomrtrika, 33, 305-325.
[30]
Ryther, J.H., Debusk, T.A. and Blakeslee, M. (1984). Cultivation and conversion of marine macroalgae (Gracilaria and Ulva). In: SERI/STR-231-2360, pp. 1–88.
[31]
Singh, A. and Olsen, S.I. (2011). A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels. Applied Energy, 88, 3548–3555.
[32]
Sluiter, A. (2006). Determination of structural carbohydrates and lignin in biomass. Vol. Version 2006. National Renewable Energy Laboratory, USA. http://www.nrel.gov/biomass/analytical_procedures.html.
[33]
Sun, Y. and Cheng, J. (2002). Hydrolysis of lignocellulosic materials for ethanol production: A review. Bioresour. Technol. 83(1): 1-11.
[34]
Sunarti, T.C., Meryandini, A., Sofiyanto, M.E. and Richana, N. (2010). Saccharification of corncob using cellulolytic bacteria for bioethanol production. BIOTROPIA, 17( 2).
[35]
Taherzadeh, M.J. and Karimi, K. (2008). Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review. International Journal of Molecular.
[36]
Tao, X., Jang, M.S., Kim, K.S., Yu, Z. and Lee, Y.C. (2008). Molecular cloning, expression and characterization of alpha-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1. Indian J Biochem Biophys.;45(5), 305-309.
[37]
Todorov, S., Giotcheva, B., Douset, X., Onno, B. and Ivanov, K. (2000). Influence of growth medium on bacteriocin production in Lactobacillus ptantarum ST31. Biotechnol. Equip; 14, 50-55.
[38]
Todorov, S.D. and Dicks, L.M.T. (2004). Effect of medium components on bacteriocin production by Lactobacillus pentosus ST15/BR, a strain isolated from beer produced by the fermentation of maize, barley and soy flour. World J. Microbiol. Biotechnol; 20, 643-650.
[39]
Trono, J.G.C. and Ganzon-Fortes, E. (1988). Philippine seaweeds. Philippines: National Book-store Inc.
[40]
Ventosa, A. and Nieto, J.J. (1995). Biotechnological applications and potentialities of halophilic microorganisms. World J. Microbiol. Biotechnol; 11, 85–94.
[41]
Wi, S.G., Kim, H.J., Mahadevan, S.A., Yang, D.J. and Bae, H.J., (2009). The potential value of the seaweed Ceylon moss (Gelidium amansii) as an alternative bioenergy resource. Bioresour Technol; 100, 6658–6660.
[42]
Xiong, W., Li, X., Xiang, J. and Wu, Q. (2008). High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl. Microbiol. Biotechnol; 78, 29–36.
[43]
Yanagisawaa, M., Nakamuraa, K., Arigab, O. and Nakasakia, K. (2011). Production of high concentrations of bioethanol from seaweeds that contain easily hydrolyzable polysaccharides. Process Biochemistry, 46, 2111–2116.
[44]
Yokoyama, M.Y. and Guimarães, O., (1975). Determinação dos teores de Na, K, O e proteínas em algas marinhas. Acta Biológica Paranaense, 4 (1/2), 19-24.
[45]
Yu, X.S., Hallett, G., Sheppard, J. and Watson, A.K. (1997). Application of the Plackett-Burman experimental design to evaluate nutritional requirements for the production of Colletotrichum coccodes spores. Appl. Microbiol. Biotechnol; 47, 301-305.
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