Biochemical Responses of Capsicum Annum Under Cadmium Stress
International Journal of Environmental Monitoring and Analysis
Volume 1, Issue 3, June 2013, Pages: 99-104
Received: Jun. 16, 2013; Published: Jul. 20, 2013
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Authors
Hima Ramachandran, Department of Environmental Sciences, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, India
Salom Gnana Thanga Vincent, Department of Environmental Sciences, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, India
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Abstract
A pot culture study was carried out to assess the antioxidant activity, lipid peroxidation and DNA damage in Capsicum annum (L.) var. Vellayani athulya, under different cadmium concentrations applied as cadmium chloride salt (10mM, 20mM, 30mM, 40mM and 50mM). Activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT) and reduced glutathione (GSH) were observed along with lipid peroxidation and DNA damage. Results showed significant changes in the activities of above antioxidants in test plants, compared to control. A significant increase in the activity of SOD, POX and decrease in the activities of CAT and GSH were observed up to 30mM concentrations. However, at 40mM concentration, the activity of antioxidants was observed to revert to the near normal, showing a sign of recovery which may be due to the inactivation of antioxidants in the presence of excess Cd. Further, in 50mM concentration, the activity of enzymes reversed to the initial condition, i.e either increased or decreased. Nevertheless, production of malondialdehyde, which is an indicator of lipid peroxidation, also showed the same pattern of change. The results of comet assay showed that the extent of DNA damage was greater in C. annum as the Cd concentration increased.
Keywords
Heavy Metal, Antioxidant Enzymes, Lipid Peroxidation, DNA Damage
To cite this article
Hima Ramachandran, Salom Gnana Thanga Vincent, Biochemical Responses of Capsicum Annum Under Cadmium Stress, International Journal of Environmental Monitoring and Analysis. Vol. 1, No. 3, 2013, pp. 99-104. doi: 10.11648/j.ijema.20130103.15
References
[1]
V. Pandhair, and B. S. Sekhon, "Reactive oxygen species and antioxidants in plants: An overview," J Plant Biochem Biotechnol, vol.15, pp.71-78, 2006.
[2]
K.J. Dietz, M. Bater, U. Kramer, "Free radicals and reactive oxygen species as mediators of heavy metal toxicity in plants: molecules to ecosystems," Berlin: Springer Verlag, pp. 73-97, 1999.
[3]
R.T. Hardyman, and B. Jacoby, "Absorption and translocation of Cd in bush beans (Phaseolus vulgaris)," Physiol Plant, vol. 61, pp. 670-674, 1984.
[4]
G.J. Wagner, "Accumulation of cadmium in in crop plants and its consequences to human health," Adv Agron, vol. 51, pp. 173-21, 1994.
[5]
K. Shah, R. G. Kumar, S. Verma, and R.S. Dubey, "Effect of cadmium on lipid peroxidation, super oxide anion generation and activities of antioxidant enzymes in rice seedlings," Plant Sci, vol.161, pp.1135-44, 2001.
[6]
T. Saitosh, K. Shinmura, S. Yamaguchi, M. Tani, S. Seki, H. Murakami, Y. Nojima, and J.Yokota, "Enhancement of OGG1 protein AP lyase activity by increase of APEX protein. DNA Repair," vol.486, pp. 31-40, 2001.
[7]
S. Sreedevi, P. N. Krishnan, and P.Pushpangadan, "Cadmium induced oxidative damage and antioxidant response in roots of black gram [Vigna mungo (L.) Hepper]," J Plant Physiol, vol.13, pp. 1-7, 2008.
[8]
A.P. Victoria, P. J. Lea, and R. A. Azevedo, "Antioxodent enzyme resposes to cadmium in radish tissues,"Phytochem, vol. 57 pp.701-710, 2001.
[9]
O.H. Lowry, N. J. Rosebrough, A.Farr, and R.J. Randall. "Protein measurement with folin-phenol reagent," J Biol Chem, vol.193 pp. 265-275, 1951.
[10]
C.Beauchamp, and I. Fridovich, "Superoxidase dismutase: improved assays and an assay applicable to acrylamide gels," Anal Biochem, vol. 44 pp. 276-287, 1971.
[11]
H.M. Hameda, and B. P. Klein,"Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts," J Food Sci, vol. 55 pp.184-185, 1990.
[12]
H. Aebi, Catalase. In: Bergmeyer H, eds. Methods of Enzymatic analysis. Weinheim: Verlag Chemie, 1983, pp. 273-277.
[13]
M.S. Moron, J.W. DePierre, and B.Manrervik, "Levels of glutathione, glutathione reductase and glutathione-s-transferase activies in rat lung and liver," Biochem Biophys Acta, vol.582 pp.67- 68,1979.
[14]
W.G. Nichans, and Samuelsson,"Formation of malonaldehyde from phospholipid arachidonate during microsomal lipid preparation," Eur J Biochem, vol. 6 pp.126-130, 1968.
[15]
N.P. Singh, M.T. McCoy, R.R. Tice, and E.L. Schneider, "A simple technique for quantitation of low levels of DNA damage in individual cells," Expt Cell Res, vol.175, pp.184-191, 1988.
[16]
A,Polle, and H. Rennenberge, "Photooxidative stress in trees. In causes of photooxidative stress and amelioration of defence system in plants. Edns. C. H. Foyer and P. M. Mullineaux, pp. 200-212, CRC Press, Boca Ration, Florida.
[17]
V. Dixit, V. Pandey, and R. Syam, "Differential oxidative responses to cadmium in roots and leaves of pea (Pisum sativum L.cv. Azad).," J Exp Bot, vol. 52, pp.1101-1109,2001.
[18]
S.M. Gallego, M. P. Benavides, and M. L. Tomaro, "Effect of heavy metal ion excess on sunflower leaves: Evidence of involvement of oxidative stress," Plant Sci, vol.121 pp. 151-159,1996.
[19]
S. Srivastava, R.D.Tripathi, and U.N.Dwivedi, "Synthesis of phytochelatins and modulation of antioxydants in response to cadmium stress in Cuscuta refiexa- An angiospermic parasite," J. Plant Physiol, vol.161, pp. 665-674, 2004.
[20]
S. Morita, M. Tasaka, H.Fujisawa, T.Ushimaru, and H.Tsuji, "A cDNA clone encoding a rice catalase isozyme," Plant Physiol, vol. 105, pp. 1015-1016,1994.
[21]
H.S. El- Beltagi, A.A.Mohamed, and M.M.Rashed, " Response of antioxidative enzymes to cadmium stress in leaves and roots of Radish (Raphanus sativus L.)," Not Sci Biol, vol.2, pp. 76-82, 2010
[22]
B.M. Shanmugaraj, H.M. Chandra, B. Srinivasan,and S. Ramalingam, "Cadmium induced Phtsio- Biochemical and Molecular Response in Brassica juncea," Inter J Phytoremediation,vol.15, pp.206-218,2012.
[23]
A.Lin, X. Zhang, M. Chen, and Q. Cao, "Oxidative stress and DNA damage induced by cadmium accumulation." J Environ Sci, vol.19, pp. 596-602, 2007.
[24]
G.Koppen, and L.Verschaeve, "The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells," Mutat Res, vol. 360, pp. 193-200,1996.
[25]
T. Gichner, Z. Patkov’a, J. Sz’akov’a, K. Demnerov’a, "Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves," Mutat Res, vol. 559, pp. 49-57.
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