Hepatic Alteration of the Egyptian Toad Amietophrynus Regularis, as Biomarker to Environmental Deterioration
International Journal of Environmental Monitoring and Analysis
Volume 3, Issue 5-1, October 2015, Pages: 22-29
Received: Aug. 11, 2015; Accepted: Sep. 6, 2015; Published: Nov. 30, 2015
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Rashad El– Sayed Mohammed Said, Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt
Samy Abde-Latif Ahmed Saber, Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt
Ali Gamal Gadel Rab, Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt
Khaled Youssef Abouel Fadl, Faculty of fish and Fisheries Technology, Aswan University, Aswan, Egypt
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Based on available toxicological studies dealt with aquatic fauna in Egypt, none of these studies considered terrestrial stage of amphibian exposure and uptake of metal from surroundings habitat. Little is known about how contaminants affect amphibians at the population level. Three sites were selected scarily in order to comprise different types and sources of pollutants from one hand, and to prove the potential of selected animals to accumulate pollutants. The common Egyptian toad Amietophrynus Regularis was collected from different localities along the river Nile. The aim of our study was to evaluate the biochemical and histopathological biomarkers in this specie to a pollution gradient caused by domestic discharges and heavy metals and to verify the adequacy of the studied animals as appropriate bioindicator in their habitat. The results of this study revealed that deterioration of natural habitat of the selected amphibian produced manifested biochemical and histopathological changes in the liver.
Amietophrynus Regularis, Histopathology, Heavy Metals
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Rashad El– Sayed Mohammed Said, Samy Abde-Latif Ahmed Saber, Ali Gamal Gadel Rab, Khaled Youssef Abouel Fadl, Hepatic Alteration of the Egyptian Toad Amietophrynus Regularis, as Biomarker to Environmental Deterioration, International Journal of Environmental Monitoring and Analysis. Special Issue: New Horizons in Environmental Science. Vol. 3, No. 5-1, 2015, pp. 22-29. doi: 10.11648/j.ijema.s.2015030501.14
A. E. Bin-Dohaish, "Effects of environmental pollution with Alkyl phenol (4- NONYL Phenol) on reproduction of Tilapia, Oreochromus Spillers (Teleosls) ,". Egyptian J. of Aquatic Research, 2008, 34, 336-355.
A. T. AbdAllah, and M. A. Moustafa, "Accumulation of lead and cadmium in the marine prosobranch Nerita Saxtilis, chemical analysis, light and electron microscopy," Environmental Pollution, 2002,116, 185-191.
C. Ward, S. Hassan, and M. Mendonça, "Accumulation and depuration of trace metals in Southern toads, Bufo Trrestris, exposed to coal combustion waste," Archives of Environmental Contamination and Toxicology, 2009, 56, 268–275.
D. Purves, "Trace elements contamination of the environment,"El-Sevier, Amsterdam. 1985.
G. Linder, and B. Grillitsch, "In Ecotoxicology of amphibians and reptiles,"Eds. D. Sparling, G. Linder, C. Bishop. Pensacola, FL. Society of Environmental Toxicology and Chemistry,, 904 . 2000.
J. P. Bryer, "Bioaccumulation and effects of metal contaminated soil on great plains toads, Bufo Cognatus,"A dissertation in environmental toxicology, Ph.D. thesis Texas Tech University. 104. 2008.
E. J. Houlahan, S. C. Findlay, R. B. Schmidt, H. A. Meyer, and L. S. Kuzmin, "Quantitative evidence for global amphibian population declines. Nature, 2000, 404, 752–755.
C. Carey, and J. C. Bryant, "Possible interrelations among environmental toxicants, amphibian development, and decline of amphibian populations,"Environmental health perspectives, 1995, 103(14), 13–17.
M. A. Abdel-Satar, "Quality of river Nile sediment from Idfo to Cairo," Egyptian J.of Aquatic Research, 2005, 31(2), 182-199.
M. A. Abdel-Satar, "Water quality assessment of River Nile from Idfo to Cairo," Egyptian J. of Aquatic Research, 331(2), 200-223.
A.G.M. Osman, and W. Kloas, "Water quality and heavy metal monitoring in water, sediments, and tissues of the African catfish Clarias Gariepinus (Burchell, 1822) from the river Nile," Egypt. J. of Environmental Protection, 2010, 1(4): 389-400.
A.G.M. Osman, A.M. Abd El Reheem, K.Y. AbuelFadl, and A.G. GadEl-Rab, "Enzymatic and histopathological biomarkers as indicators of aquatic pollution in fishes. Natural Science , 2010, 2(11): 1302-1311.
A.G.M. Osman, "Biomarkers in Nile tilapia Oreochromis Niloticus Niloticus (Linnaeus, 1758) to assess the impacts of river Nile pollution: bioaccumulation, biochemical and tissues biomarkers,"J. of Environmental Protection, 2012, 3: 966-977.
R. Andrews, and J. Levey, "Investigations into the causes of amphibian malformations in the Lake Champlain basin of New England," Vermont department of environmental conservation R.A.LaRosa Environmental Laboratory 103 S. Main St. Waterbury, VT 05671 In Collaboration with: Nathaniel Shambaugh – VT Department of Agricultural, Food & Markets Douglas Fort – Fort Environmental Laboratories James Andrews – Middlebury College, final report, 2003.
B. Bulog, K. Mihajl, Z. Jeran, and J. M. Toman, "Trace element concentrations in the tissues of Proteus Anguinus (Amphibia, Caudata,) and the surrounding environment," Water, Air and Soil Pollution, 2002, 136, 47–163.
Q. S. Luo, C. M. Plowman, M. S. Hopfer, and F. W. Sunderman, "Embryo–toxicity and teratogenicity of Cu2+ and Zn2+ for Xenopus Laevis, assayed by the Fetax procedure. Annals of Clinical and Laboratory Science, 1993, 23(2), 111-123.
L. C. Rowe, M. O. Kinney, and D. J. Congdon, "Oral deformities in tadpoles of the bullfrog (Rana Catesbeiana) caused by conditions in a polluted habitat," Copeia, 1998, 244–246.
L. C. Rowe, M. O. Kinney, P. A. Fiori, and D. J. Congdon, "Oral deformities in tadpoles (Rana Catesbeiana) associated with coal ash deposition: effects on grazing ability and growth," Freshwater Biology, 1996, 36, 723–730.
E. M. A. Ramadan, and A. E. Al–Ashkar, "The Effect of different fertilizers on the heavy metals in soil and tomato plant," Australian J. of Basic and Applied Sciences, 2007, 1(3), 300–306.
N. T. Basta, R. J., Ryan, and L. R. Chaney, "Heavy metal and trace element chemistry in residual-treated soil,": a review of impacts on metal bioavailability and sustainable land application. Environmental Quality, 2005, 34 (1), 49-63.
K. Lock, and R. C. Janssen, "Influence of aging on copper bioavailability In soils," Environmental Toxicology and Chemistry, 2001, 22(5), 1162-1166.
APHA "Standard methods for the examination of water and wastewater,"APHA, AWWA, WPCE, N. Y. Washington, 2005.
B. C. Sekomo, E. Nkuranga, L. D. P. Rousseau, and L. P. N. Lens, "Fate of Heavy Metals in an Urban Natural Wetland: The Nyabugogo Swamp (Rwanda) ,"Water, Air and Soil Pollution,2011,214, 321–333.
L. S. Venne, G. P. Cobb, G. Coimbatore, L. M. Smith, and S. T. McMurry, "Influence of land use on metal concentrations in playa sediments and amphibians in the Southern High Plains," Environmental Pollution, 2006, 144(1),112–118.
A. B. N. Jose, M. Crapez, J. J. McAlister, and C. G. Vilela, "Concentration and bioavailability of heavy metalsin sediments from Niterói Harbour (Guanabara Bay/S.E. Brazil) ,"Coastal Research, 2005, 21(4), 811-817.
APHO "Standards methods for examination of water and waste water," (American Public Health Organization), 18thedn, 1992.
J. D. Bancroft, and A. Stevens, "Theory and practice of histological techniques," 3rd ed. Churchill Livingstone, Edinburgh &New York.766p. 1996.
W. McDaniel, "Sample preparation procedure for petrochemical determination of total recoverable elements in biological tissues in "revision 1.0 Environmental monitoring systems laboratory. U.S environmental protection agency, 1991, 23–29.
A. T. AbdAllah, "Effects of dissolved lead and copper on the fresh water prosobranch Lanistes Carinatus." Malacologia, 2006, 48, 27-34.
A. T. AbdAllah, and M. A. Moustafa, "Accumulation of lead and cadmium in the marine prosobranch Nerita Saxtilis, chemical analysis, light and electron microscopy," Environmental Pollution, 2002,116, 185-191.
R. Abdel Wahaab, and M. I. Badawy, "Water quality assessment of the river Nile system," An overview. 0Biochemical and Environmental Sciences, 2004, 17, 87-100.
A. J. M. Baker, "Accumulators and excluders-strategies in the response of plants to heavy metals," Plant Nutrition, 1981, 3(1-4), 643-654.
M. I. Abdel-Hamid, S. A. Shaaban-Dessouki, and O. M. Skulberg,"Water quality of the river Nile in Egypt. 1. Physical and chemical characteristics,"Archiv für Hydrobiologie, 1992, 90, 283-310.
M. I. Abdel-Hamid, S. A. Shaaban-Dessouki, and O. M. Skulberg,"Water quality of the river Nile in Egypt. 1. Physical and chemical characteristics,"Archiv für Hydrobiologie, 1992, 90, 283-310.
A. M. Abdel-Halim, "Studies on the Physico-chemical changes of the river Nile at the region from Isna to El Kanater El-Khyria, Egypt,” 1993, M.Sc. Thesis, faculty of science, Alexandria University, Egypt.
M. A. Abdel-Satar, and A. A. Elewa, " Water quality and environmental assessments of the river Nile at Rossetta branch, the second international conference and exhibition for life and environment, 2001, 3-5 April, 136-164.
M. M. El–Sheekh, M. A. I. Deyab, S. S. Desouki, and M. Eladl, "Phytoplankton compositions as a response of water quality in El Salam canal Hadous drain and Damietta branch of the river Nile Egypt. Pakistan J.of Botany, 42(4), 2621-2633.
S. A. Shehata, and S. A. Badr, "Effectof Nile River water quality on algal distribution at Cairo, Egypt," Environment International, 1985, 11(5), 465-474.
WHO "Background document for development of WHO guidelines for drinking-water Quality. Nitrate and Nitrite in drinking water," (World Health Organization). 2011.
F. Punzo, and S. Law, "Effect of nitrate-related compounds on growth, survival and hematological responses in tadpoles of the Cuban tree frog, Osteopilus Septentrionalis (Boulenger). Environmental Biology200627(2), 187-190.
WHO "Guidelines for Drinking-water Quality. Incorporating first addendum (World Health Organization). Recommendations,3rd Ed. 2006.
E. M. M. El Bouraie, E. A. A. Barbary, M. M. Yehia, and A. E. Motawea, "Heavy metal concentrations in surface river water and bed sediments at Nile Delta in Egypt," Sau, 2010, 61(1), 1–12.
K. Simkiss, and G. M. Taylor, "Metal fluxes across the membranes of aquatic organisms," CRCCrit Revs. Aquatic Science, 1989, 1, 173-188.
R. J. P. Williams, "Natural selection of the chemical elements," Proceedings of the Royal Society of London. Series B, Biological Sciences, 1981, 213(1193), 361-397.
R. J. P. Williams, "Physico–chemical aspects of inorganic element transfer through membranes," Philosophical Transactions of the Royal Society B, 1981, 294, 57–74.
H. D. J. Phillips, and S. P. Rainbow, "Biomonitoring of trace aquatic contaminants," Elsevier Applied Science, 371. 1993.
S. J. Stohs, and D. Bagchi, "Oxidative mechanisms in the toxicity of metal ions," Free Radical Biology & Medicine, 1995, 18(2), 321-336.
Z. Varanka, I. Rojik, J. Nemcsok, and M. Abraham, "Biochemical and morphological changes in Carp (Cyprinus Carpio, L) following exposure to copper sulphate and tannic acid," Comparative Biochemistry and Physiology C, 128, 467-478.
A. Sánchez-Chardi, C. A. O. Ribeiro, and J. Nadal, "Metals in liver and kidneys and the effects of chronic exposure to pyrite mine pollution in the shrew Crocidura Russula inhabiting the protected wetland of Doñana," Chemosphere, 2009, 76(3), 387–394.
V. F. Doherty, O. O. Ogunkuade, and U. C. Kanife, "Biomarkers of oxidative stress and heavy metal levels as Indicators of environmental pollution in some selected fishes in Lagos, Nigeria ," American-Eurasian J. of Agricultural and Environmental Sciences, 2010, 7 (3), 359-365.
S. Roy, and S. Bhattacharya, "Arsenic-induced histopathology and synthesis of stress proteins in liver and kidney of Channapunctatus,"Ecotoxicology and Environmental Safety, 2006, 65, 218-229.
A. Alne-na-ei, "The illegal fish farms in the Egyptian Delta: External lesions frequency, liver histopathology and heavy metals concentrations in the muscle tissue," Egyptian J. of Aquatic Biology and Fisheries, 1998, 2(4), 119-144.
M. S. Zaki, S. O. Mostafa, O. M. Fawzi, M. Khafagy,and F. S. Bayumi, "Clinicopathological, biochemical and microbiological change on Grey Mullet exposed to cadmium chloride," American Eurasian J. of Agricultural and Environmental Sciences, 2009, 5(1), 20-23.
C. Agius, and R. J. Roberts, "Melano-macrophage centres and their role in fish pathology," Fish Diseases, 2003,26, 499-509.
A. Păunescu, C. M. PonepalV. T. Grigorean, and M. Popscu, "Histopathological changes in the liver and kidney tissues of marsh frog (Pelophylaxridibundus) induced by the action of Talstar 10EC insecticide," AnaleleUniversitatii din Oradea, FasciculaBiologie, 2012,19(1).
C. M. H. Ferreira, "Can fish liver melanomacrophages be modulated by xenoestrogenic and xenoandrogenic pollutants?" Experimental studies on the influences of temperature, sex, and ethynylestradiol, using the platyfish as the model organism. Dissertação de Candidaturaaograu de Mestreem Contaminação e Toxicologia Ambiental submetidaao Instituto de Ciências Biomédicas de Abel Salazar e à Faculdade de Ciências, da Universidade do Porto, M.Sc.thesis, p53. 2010.
N. C. Bols, J. L. Brubacher, R. C. Ganassin, and L. E. J. Lee, "Ecotoxicology and innate immunity in fish," Developmental & Comparative Immnunology, 2001, 25, 853-873.
C. M. Couillard, and P. V. Hodson"Pigmented macrophage aggregates: A toxic response in fish exposed to bleached-kraft mill effluent? ," Environmental Toxicology and Chemistry, 1996, 15, 1844–1854.
I. D. S. I. P. Thilakaratne, J. D. McLaughlin, and D. J. Marcogliese, "Effects of pollution and parasites on biomarkers of fish health in spottail shiners Notropishudsonius(Clinton) ," Fish Biology, 2007, 71, 519-538.
N. S. Loumbourdis, "Liver histopathologic alterations in the frog Ran a Ridibunda from a small river of northern Greece. Archiev Environmental of Contamination Toxicology, 2007, 53, 418-425.
R. E. M. Said, "Ecological and biological studies on some marine annelids inhabiting the Red Sea, Egypt.,"M.Sc.Thesis, Al-AzharUniv, Assiut branch, Fac.Sc, Zoology Dept. 2009.
B. E. Brown, "The form and function of metal-containing ‘granules," in invertebrate tissues Biological Reviews, 1982, 57, 621-667.
J. S. H. Pullen, and P. S. Rainbow, "The composition f pyrophosphate heavy metal detoxification granules in barnacles,"Experimental Marine Biology and Ecology, 1991, 150, 249–266.
P. A. Vesk, and M. Byrne, "Metal levels in tissue granules of the freshwater bivalve Hyridella Depressa (Unionida) for biomonitoring: the importance of cryopreservation," The Science of the Total Environment, 1999, 225, 219–29.
M. G. Taylor, and K. Simkiss, "Structural and analytical studies on metal ion–containing granules," In: Mann S, Webb J, Williams RJP, editors. Biomineralisation: Chemical and Biochemical Perspectives. Weinheim: VCH Publishers, 1989, 427–460.
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