The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library
International Journal of Environmental Monitoring and Analysis
Volume 7, Issue 2, April 2019, Pages: 48-55
Received: Jan. 3, 2019;
Accepted: Jan. 31, 2019;
Published: Sep. 10, 2019
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Huijie Liu, Guangzhou Military Area Environmental Monitoring Station, Guangzhou, China
Yun Tian, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.
The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library, International Journal of Environmental Monitoring and Analysis.
Vol. 7, No. 2,
2019, pp. 48-55.
Pan Y, Chen J, Zhou H, et al. 2017. Vertical distribution of dehalogenating bacteria in mangrove sediment and their potential to remove polybrominated diphenyl ether contamination. Marine Pollution Bulletin, 124, 1055–1062.
Priya G, Lau N S, Furusawa G, et al. 2019. Metagenomic insights into the phylogenetic and functional profiles of soil microbiome from a managed mangrove in Malaysia. Agri Gene, 9, 5–15.
Hamzah T N T, Lee S Y, Hidayat A, et al. 2018. Diversity and characterization of endophytic fungi isolated from the tropical mangrove species, rhizophora mucronata, and identification of potential antagonists against the soil-borne fungus, fusarium solani. Frontiers in Microbiology, 9, 1707.
Alongi D M. 1988. Bacterial productivity and microbial biomass in tropical mangrove sediments. Microbial Ecology, 15 (1), 59-79.
Rappe M S, Giovannoni S J. 2003. The uncultured microbial majority. Annual Review of Microbiology, 57, 369-94.
Basak P, Pramanik A, Sengupta S, et al. 2016. Bacterial diversity assessment of pristine mangrove microbial community from Dhulibhashani, Sundarbans using 16S rRNA gene tag sequencing, Genomics Data, 7, 76-78.
Rocha L L, Colares G B, Nogueira V L R, et al. 2016. Distinct Habitats Select Particular Bacterial Communities in Mangrove Sediments. International Journal of Microbiology, 2016, 1-6.
Handelsman J. 2004. Metagenomics: application of genomics to uncultured microorganisms. Microbiology and Molecular Biology Reviews, 68 (4), 669-85.
Liu M, Yu Z, Yu X, et al. 2017. Invasion by cordgrass increases microbial diversity and alters community composition in a mangrove nature reserve. Frontiers in Microbiology, 8, 2503.
Chen X Y, Lin P, Lin Y M. 1996. Mating systems and spontaneous mutation rates for chlorophyll-deficiency in populations of the mangrove Kandelia candel. Hereditas, 125 (1), 47-52.
Liu H, Tian Y, Zheng T, et al. 2008. Studies of glucosidase activities from surface sediments in mangrove swamp. Journal of Experimental Marine Biology and Ecology, 367 (2), 111-117.
Liang J B, Chen Y Q, Lan C Y, et al. 2007. Recovery of novel bacterial diversity from mangrove sediment. Marine Biology, 150 (5), 739-747.
Li M, Gu J D. 2016. The diversity and distribution of anammox bacteria in the marine aquaculture zones. Appl Microbiol Biotechnol, 100, 8943-8953.
Tian Y, Luo Y R, Zheng T L, et al. 2008. Contamination and potential biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments of Xiamen, China. Marine Pollution Bulletin, 56 (6), 1184-1191.
Tian Y, Liu H J, Zheng T L, et al. 2008. PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary, China. Marine Pollution Bulletin, 57 (6-12), 707-15.
Zhou Z, Meng H, Liu Y, et al. 2017. Stratified bacterial and archaeal community in mangrove and intertidal wetland mudflats revealed by high throughput 16S rRNA gene sequencing. Frontiers in Microbiology, 8, 2148.
Ward D M, Weller R, Bateson M M. 1990. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature, 345, 63-65.
Haldar S, Nazareth S W. 2018. Taxonomic diversity of bacteria from mangrove sediments of Goa: metagenomic and functional analysis. 3 Biotech, 8, 436.
Paingankar M S, Deobagkar D D. 2018. Pollution and environmental stressors modulate the microbiome in estuarine mangroves: a metagenome analysis. Current Science, 115 (8), 1525-1535.
Cottrell M T, Kirchman D L. 2000. Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Applied and Environmental Microbiology, 66 (4), 1692-1697.