Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory
Science Journal of Clinical Medicine
Volume 7, Issue 2, March 2018, Pages: 20-24
Received: Mar. 9, 2018; Accepted: Apr. 2, 2018; Published: May 11, 2018
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Authors
Messele Admassie, Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia
Estifanos Tsige, Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia
Meseret Chanie, Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia
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Abstract
Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.
Keywords
Wound Infections, Bacterial Pathogens, Polymicrobial, Arsho
To cite this article
Messele Admassie, Estifanos Tsige, Meseret Chanie, Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory, Science Journal of Clinical Medicine. Vol. 7, No. 2, 2018, pp. 20-24. doi: 10.11648/j.sjcm.20180702.12
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Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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