Paralytic Toxin Profiles of Xanthid Crab Atergatis Floridus Collected on Reefs of Ishigaki Island, Okinawa Prefecture, Japan and Camotes Island, Cebu Province, Philippines
Science Journal of Clinical Medicine
Volume 3, Issue 5, September 2014, Pages: 75-81
Received: Aug. 1, 2014; Accepted: Aug. 26, 2014; Published: Sep. 20, 2014
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Authors
Manabu Asakawa, Department of Biofunctional Science and Technology, Food Science and Biofunctions Division, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
Shintaro Tsuruda, Department of Biofunctional Science and Technology, Food Science and Biofunctions Division, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
Yasuyuki Ishimoto, Department of Biofunctional Science and Technology, Food Science and Biofunctions Division, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
Michitaka Shimomura, Kitakyushu Museum of Natural History & Human History, Kitakyushu, Fukuoka 805-0071, Japan
Kazuo Kishimoto, Okinawa Prefectural Fisheries Research and Extention Center, Ishigaki Branch, Ishigaki, Okinawa 907-0453, Japan
Yasuo Shida, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Mercy Barte-Quilantang, Institute of Fish Processing Technology, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, 5023 Iloilo, Philippines
Gloria Gomez-Delan, College of Fisheries Technology, Cebu Technological University - Carmen, Cebu Campus, 6005, Cebu, Philippines
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Abstract
Attempts were made to assess the toxicity and to analyze paralytic toxin profiles of xanthid crab Atergatis floridus collected on two reefs on the left- and the right-side, tentatively designated as site A and B, separated by the passage at the outside of Kabira Bay in Ishigaki Island, Okinawa Prefecture, Japan in comparison with those of the same crabs from Camotes Island off the eastern coast of Cebu Island, Cebu Province, Philippines. They were dissected into 4 parts; carapace, viscera, appendage and muscle of appendage. Muscle of appendage was highly toxic, and the maximum toxicity of 4,641±972MU/g as paralytic shellfish poison (PSP) was recorded in the specimens from the right-side reef (site B). Toxicity assays showed that all of them were toxic irrespective of the crab-collecting years, locations, and tissues, and in addition to these, there seemed to be marked narrow regionality and individual variation of toxicity and toxin profiles. Toxicity of Ishigaki specimens was seemed to be higher than that of Camotes specimens. Toxin profiles of the viscera of A.floridus were examined by high performance liquid chromatography-fluorescent detection (HPLC-FLD) analysis. In the viscera of A.floridus from site A in June, 2007, relative abundances (mole %) of carbamoyl-N-hydroxyneosaxitoxin (hyneoSTX), neosaxitoxin (neoSTX), and saxitoxin (STX) were high (98%), and only 2% of gonyautoxin 2(GTX2) were contained in addition to similar amounts (3%) of decarbamoylsaxitoxin (dcSTX). Its viscera from site B in the same month possessed GTX2 (36%) and STX group (63 %) predominantly, and only 1% of GTX1 was contained in addition to similar amount (2%) of STX. Their viscera possessed STX group as the major components (89%) along with the GTX4 (11%) as the minor. On the other hand, PSP compositions of the viscera of Camotes specimen resembled to that of the viscera from the specimens on site A in Kabira Bay with higher GTX4 but lower hyneoSTX. A solitary outstanding difference of toxin profiles in both crabs was the occurrence of tetrodotoxin (TTX) in the Camotes specimen due to the results of HPLC-FLD and gas chromatography-mass spectrometry (GC-MS) analysis.
Keywords
Xanthid Crab, Atergatis floridus, Paralytic Shellfish Poison, Tetrodotoxin, Ishigaki Island, Camotes Island, Philippines, HPLC-FID, GC-MS, Gonyautoxin, Saxitoxin
To cite this article
Manabu Asakawa, Shintaro Tsuruda, Yasuyuki Ishimoto, Michitaka Shimomura, Kazuo Kishimoto, Yasuo Shida, Mercy Barte-Quilantang, Gloria Gomez-Delan, Paralytic Toxin Profiles of Xanthid Crab Atergatis Floridus Collected on Reefs of Ishigaki Island, Okinawa Prefecture, Japan and Camotes Island, Cebu Province, Philippines, Science Journal of Clinical Medicine. Vol. 3, No. 5, 2014, pp. 75-81. doi: 10.11648/j.sjcm.20140305.11
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