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A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array
International Journal of Nutrition and Food Sciences
Volume 3, Issue 2, March 2014, Pages: 14-18
Received: Nov. 25, 2013; Published: Jan. 20, 2014
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Authors
Ki-Eun Lee, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
Woo-Seog Jeong, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
Seong-In Lim, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
Se-Ryun Kim, Panagene Incorporated, Daejon 305-510, South Korea
Yong-Kuk Kwon, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
Jin-San Moon, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
Soo-Yeon Cho, Animal, Plant Quarantine Agency, Seoul regional office, Seoul 157-843, South Korea
Jae-Young Song, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
Dong-Jun An, Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea
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Abstract
E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks.
Keywords
Escherichia coli O157, Peptide Nucleic Acid Array, Food Poisoning
To cite this article
Ki-Eun Lee, Woo-Seog Jeong, Seong-In Lim, Se-Ryun Kim, Yong-Kuk Kwon, Jin-San Moon, Soo-Yeon Cho, Jae-Young Song, Dong-Jun An, A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array, International Journal of Nutrition and Food Sciences. Vol. 3, No. 2, 2014, pp. 14-18. doi: 10.11648/j.ijnfs.20140302.11
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