Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant
American Journal of Materials Synthesis and Processing
Volume 3, Issue 1, March 2018, Pages: 1-6
Received: Sep. 28, 2017;
Accepted: Oct. 18, 2017;
Published: Jan. 18, 2018
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Rethinam Senthil, Biological Materials Lab, Central Leather Research Institute, Chennai, India
Sathyaraj Weslen Vedakumari, Faculty of Allied Health Science, Chettinad Academy of Research & Education, Chennai, India
Thotapalli Parvathaleswara Sastry, Biological Materials Lab, Central Leather Research Institute, Chennai, India
In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.
Sathyaraj Weslen Vedakumari,
Thotapalli Parvathaleswara Sastry,
Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant, American Journal of Materials Synthesis and Processing.
Vol. 3, No. 1,
2018, pp. 1-6.
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