Mass Spectrometry Analysis of Isotopic Abundance of 13C, 2H, or 15N in Biofield Energy Treated Aminopyridine Derivatives
American Journal of Physical Chemistry
Volume 4, Issue 6, December 2015, Pages: 65-70
Received: Oct. 26, 2015;
Accepted: Nov. 26, 2015;
Published: Dec. 22, 2015
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Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, USA
Alice Branton, Trivedi Global Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, USA
Gopal Nayak, Trivedi Global Inc., Henderson, USA
Gunin Saikia, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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2-Aminopyridine (2-AP) and 2,6-diaminopyridine (2,6-DAP) are two derivatives of aminopyridines that act as an important organic intermediates, mostly used in medicines, dyes and organic sensors. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, or 15N/14N, in aminopyridine derivatives using gas chromatography-mass spectrometry (GC-MS). The 2-AP and 2,6-DAP samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to Mr. Trivedi’s biofield energy treatment. The GC-MS spectra of 2-AP and 2,6-DAP showed five and six m/z peaks respectively due to the molecular ion peak and fragmented peaks of aminopyridine derivatives. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N were calculated for both the derivatives and significant alteration was found in the treated samples as compared to the respective control. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N in treated samples of 2-AP was decreased by 55.83% in T1 and significantly increased by 202.26% in T4. However, in case of 2,6-DAP, the isotopic abundance ratio of 2H/1H, 13C/12C, and 15N/14N, in the treated sample showed a significant increase (up to 370.54% in T3) with respect to the control. GC-MS data suggested that the biofield energy treatment on aminopyridine derivatives had significantly altered the isotopic abundance of 2H, 13C, or 15N in the treated 2-AP and 2,6-DAP as compared to the control.
Biofield Energy Treatment, 2-Aminopyridine, 2,6-Diaminopyridine, Gas Chromatography-Mass Spectrometry
To cite this article
Mahendra Kumar Trivedi,
Mass Spectrometry Analysis of Isotopic Abundance of 13C, 2H, or 15N in Biofield Energy Treated Aminopyridine Derivatives, American Journal of Physical Chemistry.
Vol. 4, No. 6,
2015, pp. 65-70.
Copyright © 2015 Authors retain the copyright of this article.
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Muccio Z, Jackson GP (2009) Isotope ratio mass spectrometry. Analyst 134: 213-222.
Rieley G (1994) Derivatization of organic-compounds prior to gas-chromatographic combustion-isotope ratio mass-spectrometric analysis: Identification of isotope fractionation processes. Analyst 119: 915-919.
Weisel CP, Park S, Pyo H, Mohan K, Witz G (2003) Use of stable isotopically labeled benzene to evaluate environmental exposures. J Expo Anal Environ Epidemiol 13: 393-402.
Hoefs J (2009) Stable Isotope Geochemistry, Isotope Fractionation Processes of Selected Elements. Springer-Verlag Berlin Heidelberg.
May BCH, Zorn JA, Witkop J, Sherrill J, Wallace AC, et. al (2007) Structure-activity relationship study of prion inhibition by 2-aminopyridine-3, 5-dicarbonitrile-based compounds: Parallel synthesis, bioactivity, and in vitro pharmacokinetics. J Med Chem 50:65-73.
Coleman MD (2010) Human Drug Metabolism. Role of Metabolism in Drug Toxicity. John Wiley & Sons, Ltd.; New York.
Araki Y, Andoh A, Fujiyama Y, Hata K, Makino J (2001) Application of 2-aminopyridine fluorescence labeling in the analysis of in vivo and in vitro metabolism of dextran sulfate sodium by size-exclusion high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 753: 209-215.
Korenke AR, Rivey MP, Allington DR (2008) Sustained-release fampridine for symptomatic treatment of multiple sclerosis. Ann Pharmacother 42: 1458-1465.
New Drugs: Fampridine. (2011) Australian Prescriber (34): 119-123.
Gonzalez Cabrera D, Douelle F, Younis Y, Feng TS, Le Manach C, et al. (2012) Structure activity relationship studies of orally active antimalarial 3, 5- substituted 2-aminopyridines. J Med Chem 55: 11022-11030.
Naixing W, Boren C, Yuxiang O (1993) Synthesis of N-2,4,6-trinitrophenyl-N’- 2, 4, dinitrobenzofuroxano-3, 5-dinitro-, G-diaminopyridine. J Energ Mater 11: 47-50.
Schwalbe CH, Williams GJB, Koetzle TF (1987) A neutron diffraction study of 2, 6-Diaminopyridine at 20K. Acta Cryst C 43: 2191-2195.
Freeman HG, Gillern MF, Smith HA (1976) Rapid curing, hydrophilic resin compositions. US 3947425 A.
Dickson SJ, Paterson MJ, Willans CE, Anderson KM, Steed JW (2008) Anion binding and luminescent sensing using cationic ruthenium(II) aminopyridine complexes. Chem Eur J 14: 7296-7305.
Trivedi MK, Patil S, Tallapragada RM (2012) Thought intervention through bio field changing metal powder characteristics experiments on powder characteristics at a PM plant. Future Control and Automation LNEE 173: 247-252.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
Patil S, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
Barnes PM, Powell-Griner E, McFann K, Nahin RL (2004) Complementary and alternative medicine use among adults: United States, 2002. Adv Data 343: 1-19.
Smith BC (2011) Fundamentals of Fourier transform infrared spectroscopy, CRC Press, Taylor and Francis Group, Boka Raton, New York.
Mook W, Vries J (2003-2004) Environmental isotopes in the hydrological cycle principles and applications. International atomic energy agency, Vienna, 1-271.