Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant
Agriculture, Forestry and Fisheries
Volume 4, Issue 4, August 2015, Pages: 173-178
Received: Jun. 6, 2015; Accepted: Jun. 21, 2015; Published: Jul. 9, 2015
Views 5194      Downloads 149
Sammaiah D., Department of Botany, Govt. Degree College, Huzarabad, (T.S)
Odelu G., Department of Botany, Govt. Degree College, Jammikunta, (T.S)
Venkateshwarlu M., Department of Botany, Kakatiya University, Warangal, (T.S)
Srilatha T., Department of Botany, Govt. Degree & PG College for Women, Warangal, (T.S)
Anitha Devi U., Department of Botany, Govt. Degree & PG College for Women, Karimnagar, (T.S)
Ugandhar T., Department of Botany, SRR Govt. Degree & P.G College, Karimnagar, (T.S)
Article Tools
Follow on us
The present study was conducted with the aim of evaluating some of the factors that influence induction and plantlet regeneration of somatic embryos in Mentha arvensis ( L) var piperascens Holmes (menthol or Japanese mint)since there are no available reports on Somatic embryogenesis and regeneration of plantlet in this medicinally important plant species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog medium fortified with (0.5-5.0mg/L) Napthlene acetic acid (NAA)+(0.5 mg/L)Thidizuron(TDZ). High frequency of somatic embryo formation was found at (2.5mg/L) NAA+ (0.5mg/L) TDZ in leaf explants respectively, Secondary somatic embryogenesis was also observed when primary somatic embryos were sub cultured same somatic embryo induction medium well developed cotyledonary stage embryos were germinated on MS medium supplemented with (0.5mg/L) (NAA) + (0.5-5.0mg/L)TDZ maximum 80% of somatic embryos germination and plant let formation was found at (2.5 mg/L) NAA+ (0.5 mg/L) (TDZ). The post translation survival rate of plants was 80% plants and flowers formation were morphological similar to the mother plants.
Leaf Explants NAA (Napthlene Acetic Acid), TDZ (Thidizuron), Mentha arvensis (L), Somatic Embryos and Regeneration
To cite this article
Sammaiah D., Odelu G., Venkateshwarlu M., Srilatha T., Anitha Devi U., Ugandhar T., Plantlet Regeneration of Somatic embryos from Leaf Explants of Mentha arvensis(L.) A medicinally important Plant, Agriculture, Forestry and Fisheries. Vol. 4, No. 4, 2015, pp. 173-178. doi: 10.11648/j.aff.20150404.14
Ammirato, P.V. (1983). The regulation of Somatic embryos development in plant cell cultures, suspension cultures technique and hormone requirements. Bio. Technol., 1: 68-74.
Ammirato, P.V. (1987).Organisation events during Somatic embryogenesis. In: Plant Tissue & Cell Culture, (Eds.). Green, C.E., Somers, D.A., Hackett, W.P. & Biesboer, D.D. Alan R. Liss, Inc., New York, pp. 57-81.
Binzel, M. L., Sanhla, A. N., Joshi, S. and Sankhla, D., (1996).:In vitro regeneration in chilli pepper (Capsicum annuum L.) from half-seed explant. Plant Growth Regulation, 20: 287-293.
Budavari, S., O’Neil, M. J., Smith, A. and Heckelmen, P. E. (1989).The Merk Index.An Encyclopedia of Chemicals, Drugand Biologicals.(11thEdition). Merck and Co., Rahway.
Caissard,J.C., Faure, O., Jullien, F., Colson, M and Perrin, A.(1996).Adirect regeneration in vitro and transient GUS expression in Mentha piperita. Plant Cell Rep.16: 67-70.
Cellarova, E.(1992).Micro-propagation of Mentha L.; In: Biotechnology in agriculture and forestry (ed.) Y P S Bajaj (Berlin, Heidelberg: Springer-Verlag) vol. 19, pp. 262-276
Chand, S. and Singh, A.K. (2001).Direct somatic embryogenesis from zygotic embryos of a timber-yielding leguminous tree. Hardwickia binata Roxb.Curr. Sci., 80: 882-888.
Chen, L.-J. and Luthe, D.S. (1987) Analysis of proteins from embryogenic and non-embryogenic rice (Oryza sativa L.) calk Plant Sci. 48, 181-188.
Choi, J., Liu, L.S., Borkid, C. and Sung, Z.R.(1987). Cloning of developmentally regulated genes” Proc.Nat Acad. Sci 84: 1906- 1910.
Choi,Y.E., Kim,J.W and Soh, W.Y.(1987) Somatic embryogenesis and plant regeneration from suspension cultures of Acanthopanax koreanum. Plant Cell Rep., 17: 84-8.
Desai,HV. Bhatt, P.N and Metha,A.R (1986) Plant Regeneration of Sapindus trifoliatus L. (Soap nut) through Somatic embryogenesis Plant Cell Rep.,3: 190-191.
Dirks, R. and Buggenum, M.V. (1989). In vitro plant regeneration from leaf explants of Cucumis melo L, J. Plant. Physiol. 132: 373-377.
Evans, D.A., Sharp, W.R. and Flick, Ch.E. (1981). Hand Book of Plant Tissue Culture Methods and Appl. in Agr.Culture. New York, London, pp: 45-113.
Garin, E., Grenier, E. and Granier G.D. (1997) Somatic embryogenesis in wild cherry (Prunusauium).Plant Cell Tissue and Org. Cult.,48: 83-91.
Gleddle, S., Keller, W. and Setterfield, G. (1983). Somatic embryogenesis and plant regeneration from leaf explants and cell suspensions of Solanum melongena (egg plants). Can. J. Bot., 61:656-665.
Gulluce, M., Sahin, F., Sokmen, M., Ozer, H., Daferera, D.,Sokmen, A., Polissiou, M., Adiguzel, A. and Ozkan, H. (2007).Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L.. Food Chemistry,103: 1449-1456.
Jelaska, S. (1974).Embryogenesis and organogenesis in Pumpkin explants.Physiol . Plant. 31: 257-261.
Karasawa, D. and Shimizu, S. (1980).Triterpene acids in callustissues from Mentha arvensis var. piperascens Agric. Biol. Chem. 44: 1203-1205.
Kathal, R., Bhatnagar, S.P. and Bhojwani, S.S (1986). Regeneration of plants from leaf explants of Cucumis meloc.v. pusasharbati. Plant Cell Reports.7: 449-451.
Kofidis, G., Bosabalidis, A. and Kokkini, S. (2006).Seasonal variations of essential oils in a linalool-rich chemotype of Mentha spicata grown wild in Greece.Journal of EssentialOil Research, 16; 469-472.
Kohlenbach, H.W. (1978).Camparative Somatic embryogenesis In: Frontiers of Plant tissue culture (Ed. Thorpe T.A.) Universal Calagry Press. pp. 59-66.
Kukreja,A. K., Dhawan, 0.P., Ahuja, P. S., Sharma, S. and Mathur,A. K.(1992). Genetic improvement of mints: On the qualitative traits of essential oil of in vitro derived clones of Japanese mint (Mentha arvensis var. piperascens Holmes).J. Essent. Oil Res., 4: 623-629.
Kumari,K.G., Ganesan,M and Jayabalan, N. (1996). Somatic organogenesis and plant regeneration in Ricinus communis. Biol. Plant.52:17-25.
Lakhmana Rao, P.V. and Singh B. (1991).Plantlet regeneration from encapsulated somatic embryos of hybrid Solanum melongena L Plant Rep.10: 7-11.
Lin M and Staba E J (1961) Peppermint and spearmint tissue cultures, callus formation and submerged culture; Lloydia (24) 139-145
Mallikarjun, N., Reena, M.J.T., Sastri, D.C. and Moss, J.P. (1996).Somatic embryogenesis in pigeon pea (Cajanus cajan). Indian J. Exptl. Biol., 34: 282-294.
Matsuoka, H. and Hinata, K. (1979).NAA–induced organogenesis and embryogenesis in hypocotyls callus of Solanum melogena. L.J. Exptl. Bot., 30: 363-370.
Mollins, M.G. and Sreenivasan, C. (1976). Somatic embryos and plantlets from on ancient clone of the Grape vine (Cv Cabernet Sauvignon) by apomixes In Vitro J.Exp. Bot., 27: 1022-1030.
Moreno, L., Bello, R., Prime-Yufera, E., Esplugues, J. (2002).Pharmacological properties of the methanol extract from Mentha suaveolens Ehrh. Phytotherapy Research. 16: 10-13.
Murashige, T. and Skoog, F.(1962).A revised medium for rapidgrowth and bioassay with tobacco tissue cultures.Physiol. Plant., 15: 473-497.
Oridate, T. and Oosawa, K.(1986).Somatic embryo genesis and plant regeneration from suspevsion callus culture in melon (Cucumis melo L.).Ja J. Breed., 36: 424 – 428.
Pandya, H. A. and Saxena, O. P. (2000). “Role of PGRs on tissue culture raised plantlets of Gladiolus, Chrysanthemum and Lily” (abstr) Role of plant growth regulators and plant biotechnology to improve growth and productivity of plants, Botany Department, Gujarat University, Ahmedabad, India.
Patel, M.B., Bhardwaj, R. and Joshi, A. (1994).Organogenesis in Vigna radiates L. Wilczek” Indian J. Exp. Biol. 29: 619- 622.
Rech,E.L. andPires, M. J. P.(1986) Tissue culture propagation of Mentha the use of axillary buds Plant Cell Rep. 5:7-18.
Reed, B. M. (1999).In vitro storage conditions for mint germplasm. Hort. Sci. 34: 350-352.
Roberts, A.V., Yokoya, K., Walker, S. and Motley, J. (1995). Somatic embryogenesis in woody plants (Eds) Jain, S., Gupta, P. Newton, R.) Kluwer Academic Publishers, The Netherlands, pp. 277-289.
Sato, S., Newell, C.Kolacz, K. and Tredo, L. (1993). Stable transformation via particle bomardment in two different soybean regeneration systems.Plant cell Rep.12: 408-413.
Saha. S Ghosh P. D and Sengupta. C (2010) “In Vitro Multiple Shoot Regeneration of Mentha piperita,” Journal of Tropical Medicinal Plants, Vol. 11, pp. 89- 92,
Sahrawat AK and Chand S (2001) Continuous somatic embryogenesis and regeneration from hypostyle segments of Psoralea corylifolia Linn: An endangered and medicinally important Fabaceae plant. Curr. Sci. 81:1328-1331.
Sharma, P. and Rajam M.V. (1995). Genotype, explant & position effects on organogenesis & Somatic embryogenesis in eggplant (Solanum melongena L.) J. Exptl. Bot., 46: 135-141.
Sharp, W.R., Sondehl, M.R., Caldas, L.S. and Maraffa, L.S. (1980). The physiology of In vitro a sexual embryogenesis Hort. Rev. 2: 268-310.
Sondahl, M.R. and Sharp W.R. (1977). High frequency of induction somatic embryos in cultured leaf explants of coffea arabica L. Z. Pflanzen Physiol., 81: 395-408.
Stuart, D.A., Nelsen, J., Strickland, S.G. and Nichol, J.W. (1985). Factors affecting developmental processes in alfa alfa cell culture. In: Tissue culture in Forestry & Agriculture. R.R. Henke, K.W. Hughes, M.P. Constantin and A. Hollaender (Eds). Pp. 58-73.
Suesmatso, N., Ootsuka, H. and Toda, M. (1986). Bull. Schizuoka.Agr. Exp.Stone. 31: 31-38.
Thirupathi1.M Srinivas.D and Jaganmohan Reddy (2013) High frequency of multiple shoots induction in Paederia foetida (L.)- A rare medicinal plant 2013 Science publishing Group 1(5):60-65
Ugandhar T. (2002). Tissue culture studies in Solanam surattense Ph.D.Thesis, Kakatiya University, Warangal, (T.S).
Van Eck,J. and Kitto,S.L. (1992).Regeneration of peppermint and orange mint from leaf disks. Plant Cell Tiss.Org. Cult., 30: 41-46.
Yadegarinia, D., L. Gachkar, M. B. Rezaei, M. Taghizadeh, S. A.Astaneh, I. and Rasooli. (2006). Biochemical activities of Iranian Mentha piperita L. and Mentha communis L., essential oils. Phytochemistry, 67, 1249-1255.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186