American Journal of Life Sciences
Volume 2, Issue 2, April 2014, Pages: 72-75
Received: Mar. 12, 2014;
Accepted: Apr. 8, 2014;
Published: Apr. 10, 2014
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Jamali Fatima, Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, Iran
Baserisalehi Majid, Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, Iran
Bahador Nima, Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran
Propolis is a resinous mixture that collects by honey bees from the plants. However, the physical character of propolis generally has been used by honey bees to protect their hive, but several beneficial properties of this compound could be considered for human being. In the present study, two propolis samples (P1&P2) were collected and subjected for extraction using different solvents. Then, their antimicrobial effects were evaluated against Salmonella typhi PTCC 1609, Pseudomonas aeruginosa PTCC 1047, Staphylococcus aureus PTCC 1112, Escherichia coli PTCC 1338, Bacillus cereus PTCC 1015, Aspergillus niger PLM 1140 and Candida albicans ATCC 1405 using Well Diffusion Agar. In addition, the bioactive compounds and functional groups of the extracts were determined by paper chromatography and Spray methods. The results obtained indicated that ethanol and methanol extracts of the propolis showed relatively more antimicrobial effect and both extracts exhibited similar responses against the antagonistic microorganisms. Bacillus cereus, Salmonella typhi , Escherichia coli and Staphylococcus aureus were sensitive whereas, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger were resistant to the extracts. E.coli was sensitive to methanol and resistant to ethanol extracts. Our finding concerning to the chemical analysis of the propolis exhibited the presence of flavonoid, tannin, steroid, alcohol and alkaloid in extracts. Overall, propolis has antimicrobial effect with different spectrum and therefore, it might consider a potent candidate for treatment of several clinical scenarios.
Antimicrobial Activity and Chemical Screening of Propolis Extracts, American Journal of Life Sciences.
Vol. 2, No. 2,
2014, pp. 72-75.
U, Atec, S. Kadriye, O. Ozant, C. Dilsah, G. Omur , S. Bekir S .Chemical composition and antimicrobial activities of four Anatolian propolis samples. Microbial research 2005. pp. 186-195.
T. Ayhan ,S. Ayla, O. T. Asli,S. Kadriye, S. Bekir . Antibacterial activity of bee propolis sample from different geographical regions of turkey against two food borne pathogens, salmonella Enteritidis and listeria monocytogenes Turk J Biol. 2011. p.35305-511
L. Selvameenal, M. Radhakrishnan, R. Balagurunathan.Antibiotic Pigment from Desert Soil Actinomycetes; Biological Activity, Purification and Chemical Screening. Indian Journal of Pharmaceutical Sciences. 2009.pp.499-404.
A. Anskota, Y .Tezka, S. Kdota. Recent progress in pharmacological research of propolis. Phytother res . 2011. pp.561-571.
E. Ghisalberti. Propolis : a review. World 1979. pp. 59-84 ,
S. Ivancajic , I.Mileusnic , D. Milosevic. In vitro antibacterial activity of propolis extracts on 12 different bacteria in condition of 3 various ph values. Arch.biol.sci,Belgrade 2010. pp. 915.934.
R. Mantovani, V. Rall , J. Batalha , A. Fernandes, Anti-coagulase-negative Staphylococcus activity of ethanolic extracts of propolis from two Brazilian regions and synergism with antimicrobial drugs by the E-test method. Journal of Venomous Animals and Toxins Including Tropical Diseases 2008. pp. 357–365.
H. Jork , W. Funk, H. Wimmer, N. Wiley. TLC Reagents & Detection methods-physical & chemical detection methods: Activation Reactions, Sequences, Reagents II, Vol 1b, illuminatiy 1994. .
K. Markham, K. Mitchell, A. Wilkins, J. Daldy , Y. lu. HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry 1996. pp. 205-211.
S. Mayuran, M.Radhakrishnan, R. Balagurunathan. Antimicrobial pigments from desert soil actinomycetes. In;proceeding of national symposium on “Recent Trends in Microbial Biotechnology”. Sri Sankara arts and Science College. 2006. pp. 35-31.
S. Castaldo, F. Capasso.Propolis, an old remedy used in modern medicine Fitoterapia 2002. pp. 1–6.
S. Nallahalli ,B. Musaiah, R. Hemagirigowda. Antimicrobial activity of propolis of trigona Sp. and Apis mellifera of Karnataka, India, Prime Journal of Microbiology Research. 2008. pp. 80-85.
P. Milena, D. Rosa, T. Hassan, T. Iva, N. Hristo, B.Vassya. Omani propolis: Chemical profiling, antibacterial activity and new propolis plant sources. Chemistry Journal. 2013. p. 158.
S. Forcing, J. Bankova. Propolis:is there a potential for the development of new drug. J Ethnopharmacaol . 2011. pp. 253-240.
H. Silvia, J. Marcos, W. Evandro, Y. Izabel, C. Dioneia. antimicrobial activity of flavonoids and sreroids isolated from two Chromolaena species. Brazilian journal of pharmaceutical scinces. 2013. P. 4. out./dez.
S. Vassya, D. Solangel , R. Cast, C. Mari. Review article. Propolis: recent advances in chemistry and plant origin. Apidologie. 2000. pp. 313-15.
Y. Park, J. Paredes-Guzman, C. Aguiar, S. Alencar, F. Fujiwara. Chemical constituents in Baccharis dracunculifolia as the main botanical origin of Southeastern Brazilian propolis Journal of Agricultural and Food Chemistry. 2004. pp. 1100–1103.
S. Yaghoubi, G. Ghorbani, R. Soleimanian Zad,sattari . antimicrobial activity of Iranian propolis and its chemical composition. DARU. 2007. pp. 45-48.
Y. Xu, L. Luo, B. Chen, Y. Fu. Recent development of chemical components in propolis. Frontiers of Biology in China. 2009. pp. 385–391.
G.Trease,W.Evans.Pharmacognosy(15thEd),Saunders company, London.20