Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items
American Journal of Life Sciences
Volume 1, Issue 6, December 2013, Pages: 267-272
Received: Nov. 18, 2013;
Published: Dec. 20, 2013
Views 3499 Downloads 241
Nishat Sarker, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Sharmin Islam, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Mehedi Hasan, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Farzana Kabir, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Md. Aftab Uddin, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts. Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with an array of bacteria ranging between 3.24×102 -6.5×109 cfu/g. Results of multiplex polymerase chain reaction (PCR) assay revealed that out of 130 isolates of E. coli screened from all categories of food items, 52 (13%) were found to be enterotoxigenic E. coli, 32 (8%) were the Shiga toxin producing E. coli isolates, 14 (3.5%) were enteropathogenic and 6 (1.5%) were found to be enteroinvasive isolates. Results of plasmid profiling of the isolates was further in agreement to the presence of different pathogenic E. coli strains.
Md. Aftab Uddin,
Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items, American Journal of Life Sciences.
Vol. 1, No. 6,
2013, pp. 267-272.
Hanashiro, A., Morita, M., Matte, G.R., Matte, M.H., Torres, E.A.F.S., (2005) Microbiological quality of selected street foods from a restricted area of Sao Paulo city, Brazil. Food Control 16, 439-444.
Akobundu, E.N.T., (1996) Packaging of street foods. Paper delivered at Workshop on Street Foods Organized by Nigerian Institute of Food Science and Technology, University of Agriculture, New Delhi.
Lee, W,H., Sakari, T., Lee, M., Hamakawa, M., Lee, S., Lee, I., (1996) An epidemiological study of food poisoning disorders in Korea and Japan. International Journal of Food Microbiology 29 (2-3), 141-148.
Bryan, F.L., Michanie, S.C., Alvare, P.Z., Paniagua, A., (1988) Critical control points of street-vended foods in dominican republic. Journal of Food Protection 51, 373-383.
Muinde, O.K., Kuria, E., (2005) Hygenic and sanitary practices of vendors of street foods in Nairobi, Kenya. African Journal of Food, Agriculture, Nutrition and Development 5, 1-13.
Nicolas, B., Razack, B.A., Yollande, I., Aly, S., Tidiane, O.C.A., (2007) Street-vended foods improvement: Contamination mechanisms and application of food safety objective strategy: Critical review. Pakistan Journal of Nutrition 6, 1-10.
Farzana, K., Akram, M. R., Mahmood, S., (2011) Prevalence and antibiotic susceptibility patterns of some bacterial isolates from a street vended fruit product. African Journal of Microbiology Research 5 (11), 1277-1284.
Noor, R., Uddin, M.A., Hoque, M.A., Munshi, S.K., Acharjee, M., Rahman, M.M., (2013) Microbiological study of vendor and packed fruit juices locally available in Dhaka city, Bangladesh. International Food Research Journal 20 (2), 1011-1015.
Karunasagar, I., Karunasagar, I., Kumar, S.H., (2002) Molecular methods for rapid and specific detection of pathogens in seafood. Aquaculture Asia 3, 34-37.
Galane, P.M., Le Roux, M., (2001) Molecular epidemiology of Escherichia coli isolated from young South African children with diarrhoeal diseases. Journal of Health Population and Nutrition 19 (1), 31-38.
Kalantar, E., Soheili, F., Salimi, H., Dallal, S.M.M., (2011) Frequency, antimicrobial susceptibility and plasmid profiles of Escherichia coli pathotypes obtained from children with acute diarrhea. Jundishapur Journal of Microbiology 4 (1), 23-28.
Rappelli, P., Maddau, G., Mannu, F., Colombo, M.M., Fiori, P.L., Cappuccinelli, P., (2001) Development of a set of multiplex PCR assays for the Simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. The New Microbiologica 24 (1), 77-83.
Vidal, M., Kuger, E., K., Duran, C, Lagos, R., (2005) Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections. Journal of Clinical Microbiology 43, 5362-5365.
Aranda, K.R.S., Fabbricotti, S.H., Fagundes-Neto, U., Scaletsky, I.C.A., (2007) Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin producing Escherichia coli strains in Brazilian children. FEMS Microbiology Letters 267, 145-150.
Nataro, J.P., Kaper, J.B.,(1998) Diarrheagenic Escherichia coli. Clinical Microbiology Review 11, 142-201.
Munshi, S.K., Rahman, M.M., Noor, R., (2012) Detection of virulence potential of diarrheagenic Escherichia coli isolated from surface water of rivers surrounding Dhaka City. Journal of Bangladesh Academy of Sciences 36 (1): 109-122.
APHA (American Public Health Association), (1998) Standard Methods for the Examination of Water and Wastewater. 20th ed. American Public Health Association, Washington, D.C.
Feng, P., Weagant, S., Grant, M., (2002) Enumeration of Escherichia coli and the coliform bacteria. In: Bacteriological Analytical Manual, 8th ed. FAD/Center for Food Safety & Applied Nutrition.
Bauer, A.W., Kirby, W.M.M., Sherris, J.C., Tierch, M., (1966) Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45 (4), 493-496.
Ferraro, M.J., Craig, W.A., Dudley, M. N., (2001) Performance standards for antimicrobial susceptibility testing. 11th ed. NCCLS, Pennsylvania, USA.
Kado, C.I., Liu, S.T., (1981) Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145, 1365-1373.
Tessi, M.A., Aringoli, E.E., Pirovani, M.E., Vincenzini, A.Z., Sabbag, N.G., Costa, S.C., (2002) Microbiological quality and safety of ready-to-eat cooked foods from a centralized school kitchen in Argentina. Journal of Food Protection 65 (4), 636-642.
Dawson, R., Liamrandsi, S., Boccas, F.,(1996) Bangkok’s street food project. Food, Nutrition and Agriculture / Alimentation Nutrition and Agriculture, FAO Publication 17/18.
Eley, A.R., (1996) Toxic bacterial food poisoning. In: Eley, A.R., (Ed.), Microbial Food Poisoning, 2nd ed. Chapman and Hall, London, pp. 47.
Arifeen, S.E., Akhter, T., Chowdhury, H.R., Rahman, K.M., Chowdhury, E.K., Alam, N., Hoque, D.M.E., Baqui, A.H., (2005) Causes of death in children under five years of age. Availableat: http://www.measuredhs.com/pubs/pdf/FR165/09Chapter09.pdf (Accessed 13 August 2013).
Qadri, F., Svennerholm, A.M., Faruque, A.S., Sack, R.B., (2005) Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment and prevention. Clinical Microbiology Review 18, 465–483.
Rahman, F., Noor, R., (2012) Prevalence of Pathogenic bacteria in common salad vegetables of Dhaka metropolis. Bangladesh Journal of Botany. 41 (2), 159-162.
Mazumdar, K., Dutta, N.K., Dastidar, S.G., Motohashi, N., Shirataki, Y., (2006) Diclofenac in the management of E. coli urinary tract infections. In Vivo 20(5), 613-9.
Dutta, S., Hassan, M.R., Rahman, F., Jilani, M.S.A., Noor, R., (2013) Study of antimicrobial susceptibility of clinically significant microorganisms isolated from selected areas of Dhaka, Bangladesh. Bangladesh Journal of Medical Science 12 (1), 34-42.
Bennett, P.M., (2008) Plasmid encoded antibiotic resistance: Acquisition and transfer of antibiotic resistance genes in bacteria. Brazilian Journal of Pharmacology 153 (1), 347-357.
Franzolin, M.R., Alves, R.C., Keller, R., Gomes, T.A., Beutin, L., Barreto, M.L., (2005) Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil. Memórias do Instituto Oswaldo Cruz 100, 359-363.
Kaper, J.B., Nataro, J.P., Mobley, H.L., (2004) Pathogenic Escherichia coli. Nature Reviews Microbiology 2, 123-40.