In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria
American Journal of Life Sciences
Volume 1, Issue 3, June 2013, Pages: 113-116
Received: May 16, 2013;
Published: Jun. 20, 2013
Views 3103 Downloads 170
Dash B. K., Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
Sen M. K., Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
Alam M. K., Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh
The inhibitory activity of seed extracts of Achyranthes aspera, a widely used folk medicinal plant in Bangladesh, was examined using methanol, acetone, ethyl acetate and petroleum sprit as solvents against five test bacteria by disc diffusion method. Methanol extract was found to reveal significant inhibitory activity against the pathogenic B. subtilis, E. coli and K. pneumoniae. Only the methanol and ethyl acetate extracts were effective against all bacteria and the best activity was found against B. subtilis in terms of zone of clearance. The best minimum inhibitory concentration value was found by methanol extract against B. subtilis. The present study suggests that the methanol extract of seed of this plant could be a possible source of obtaining new and effective herbal medicines to treat infections; hence, it justified the ethnic use.
Dash B. K.,
Sen M. K.,
Alam M. K.,
In Vitro Inhibitory Activity of Achyranthes aspera L. Seed against Some Test Bacteria, American Journal of Life Sciences.
Vol. 1, No. 3,
2013, pp. 113-116.
Tullanithi KM, Sharmila B and Gnanendra TS. Preliminary phytochemical analysis and antimicrobial activity of Achyranthes aspera Linn. Int. J. Biol. Technol. 2010; 1(3): 35-38.
Okemo PO, Bais HP and Vivanco JM. In vitro activities of Maesa lanceolata extracts against fungal plant pathogens. Fitoterapia. 2003; 74: 312-316.
Lakshmi NPV, Kishore KK, Mohan KC, Gunesh G and Narasimha RM. Antimicrobial activity of Achyranthes aspera. Biosci. Biotechnol. Res. Asia. 2006; 3: 1-2.
Bouamama H, Noel T, Villard J, Benharref A and Jana M. Antimicrobial activities of the leaf extract of two Moroccan Cistus L species. J. Ethnopharmacol. 2006; 104: 104-107.
Valsaraj R, Pushpangadan P, Smitt UW, Andersen A and Nyman U. Antimicrobial screening of selected medicinal plants from India. J. Ethnopharmacol. 1997; 58: 75-83.
Lai PK and Roy J. Antimicrobial and chemopreventive properties of herbs and spices. Cur. Med. Chem. 2004; 11: 1451-1460.
Watt JM and Breyer-Brandwijk MG. Medicinal and Poisonous Plants of Southern and Eastern Africa, 2nd edition. E & S Livingstone. 1962; p989-1008.
Anonymous. The Wealth of India, Raw Materials. New Delhi: Publication and Information Directorate, Center for Scientific and Industrial Research (CSIR). 1985; p57.
Ratra PS and Misra KC. Seasonal variation in chemical composition of A. aspera and A. bidentata. Indian Forester. 1970; 96: 372-375.
Singleton P. Bacteria in Biology, Biotechnology and Medicine. 4th edition. New York, John Wiley and Sons Ltd. 1999.
Londonkar R, Reddy CV and Kumar AK. Potential antibacterial and antifungal activity of Achyranthes aspera L. Recent Res. Sci. Technol. 2011; 3(4): 53-57.
Narayana RK, Reddy SM, Chaluvadi MR and Krishna DR. Bioflavanoids: classification, pharmacological, biochemical effects and therapeutic potentials. Indian J. Pharmacol. 2001; 33: 2-16.
Gokhale AB, Damre AS, Kulkami KR and Saraf MN. Preliminary evaluation of anti-inflammatory and anti-arthritic activity of S. lappa, A. speciosa and A. aspera. J. Phytomed. 2002; 9(5): 433-437.
Seher G, Dilek Turgut-Balik and Nazmi G. Antimicrobial activities and some fatty acids of Turmeric, Ginger root and Linseed used in treatment of infectious diseases. World J. Agri. Sci. 2006; 2(4): 439-442.
Bauer AW, Kirby WM., Sherris JC and Turck M. Antibiotic susceptibility testing by a standardized single disc method. American J. Clin. Pathol. 1966; 45: 493-496.
NCCLS. Performance standards for antimicrobial disk susceptibility tests: Approval standard M2-A7 7th edition. Pennsylvania: Clinical and Laboratory Standards Institute. 2000.
Reiner R. Antibiotica: An Introduction. F. Hoffman (Ed.). La Roche and Co. Ltd. Switzerland. 1982; p23-30.
Ashokkumar P, Rajkumar and Kanimozhi. Phytochemical screening and antimicrobial activity from five Indian medicinal plants against human pathogens. Middle-East J. Sci. Res. 2010; 5(6): 477-482.
Varuna KM, Khan MU and Sharma PK. Review on Achyranthes aspera. J. Pharm. Res. 2010; 3(4): 714-717.
Beaulah AG, Sadiq MA and Santhi JR. Antioxidant and antibacterial activity of Achyranthes aspera: An in vitro study. Der Pharma Chemica. 2011; 3(5): 255-262.
Alam MT, Karim MM and Khan SN. Antibacterial activity of different organic extracts of Achyranthes aspera and Cassia alata. J. Sci. Res. 2009; 1: 393-398.
Elumalai EK, Chandrasekaran N, Thirumalai T, Sivakumar C, Therasa SV and David E. Achyranthes aspera leaf extracts inhibited fungal growth. Int. J. Pharm. Tech. Res. 2009; 1(4): 1576-1579.