Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production
American Journal of Life Sciences
Volume 3, Issue 1-2, January 2015, Pages: 43-47
Received: Dec. 10, 2014;
Accepted: Dec. 13, 2014;
Published: Dec. 27, 2014
Views 3474 Downloads 123
Elena Arkhipova, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Irina Alchinova, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Mikhail Karganov, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Interferon-γ (IFN-γ) is a pleiotropic cytokine with antiproliferative and immunomodulatory activities that are crucial for the regulation of immune responses. We examined a group of military pilots. The examinees were divided into 3 subgroups: ground personnel (9 persons, control group), 17 pilots with <1000 h flight time, and 12 pilots with >1000 h flight time. No differences in IFN-α serum content after induction by NDV virus were detected. The quality of reparation is in many respects genetically determined; therefore, we used peripheral blood lymphocytes from pilots for in vitro detection of a radioadaptive response (RAR), which was evaluated by the number of chromosome aberrations. The adaptive response was observed in 7 individuals of the control group (78%), in 10 pilots who had <1000 flight hours (59%), and in 4 pilots having >1000 flight hours (33%). The examined individuals were divided into 2 groups depending on the presence of RAR, and IFN-γ production after radiation was measured. It was shown that at doses 0.05 Gy or 0.5 Gy no differences between groups were detected. Exposure with these doses sequentially in 48 h interval resulted to differently directed changes: lymphocytes of individuals with RAR produced more IFN-γ than before while cells of persons without RAR made it less. The quality of adaptive mechanists evaluated by RAR may be useful for estimation of individual sensitivity to radiation during radiotherapy in oncology and in prediction of professional risk.
Flight Factors Influence on Human Lymphocyte Radioadaptive Response and Gamma-Interferon Production, American Journal of Life Sciences. Special Issue: Space Flight Factors: From Cell to Body.
Vol. 3, No. 1-2,
2015, pp. 43-47.
Cavallo D., Ursini C.L., Carelli G., Iavicoli I. et. al., “Occupational exposure in airport personnel: Characterization and evaluation of genotoxic and oxidative effects,” Toxicology, Apr., 2006, pp. 16 – 18.
Radiation Exposure and High-Altitude Flight, NCRP Commentary, 12, 1995.
Bochkov N.P., Popova N.A., Katosova L.D. and al., “Extraordinarily high level of chromosomal variability in culture human peripheral blood lymphocytes,”Genetika, vol. 35, No. 6, 1999, pp. 838-841. (in Russian)
Antoschina M.M., Fesenco E.V., Nasonova V.A., Ryabchenco N.I., “Adaptive response after preliminary irradiations of human lymphocytes,” 27th Annual Meeting of the European Society for Radiation Biology, vol. 32,1997, pp.407-408.
Mortazavi S.M.J., Mozdarani H. “The search for a possible optimum adapting dose under the optimum irradiation time scheme in cultured human lymphocytes,”International Journal of Low Radiation, vol. 3, No. 1,2006, pp.74-82.
B.S. Fedorenko, Iu.I. Voronkov, G.P. Snigireva, V.A. Shevchenko, S.V. Druzhinin, Iu.A. Akatov, V.V. Tsetlin, “Effect of space flight factors on health of cosmonauts in the near and late term after space flights,”Radiats Biol Radioecol, Nov-Dec, vol. 42, No. 6, 2002, pp.765-8. (in Russian)
Nenoi M, Wang B, Vares G., “In vivo radioadaptive response: A review of studies relevant to radiation-induced cancer risk,” Hum Exp Toxicol., 2014 Jun 12, pii: 0960327114537537. [Epub ahead of print]
David J.T.Jr., J.R. Patterson, C.Velasco-Gonzalez, E.N. Carroll, J. Trinh, D. Edwards, A.Aiyar, B. Finkel-Jimenez, and A.H. Zea, “Interferon-Gamma-Induced Nitric Oxide Inhibits the Proliferation of Murine Renal Cell Carcinoma Cells,” International Journal of Biological Sciences, vol. 8, No. 8,2012, pp. 1109-1120. doi: 10.7150/ijbs.4694
Kaffman A., O’Shea E.K., “Regulation of nuclear localization: a key to a door,” Annu. Rev. Cell Dev. Biol, vol.15, 1999, pp.291-339.
Lillemeier B.F., Koster M., Kerr I.M., “STAT1 from the cell membrane to the DNA,”EMBO J, vol. 20, No. 10, 2001, pp.2508-2517.
Gupta P., Su Z., Lebedeva I.V. et al., “mda-7/IL-24: Multifunctional cancer-specific apoptosis-inducing cytokine,” Pharmacol. Therap., vol.11, No. 3, 2006, pp.596-628.
Kawabe S., Nishikawa T., Munshi A., “Adenovirus-mediated mda-7 gene expression radiosensitizes non-small lung cancer cells via TP53-independent mechanisms,” Molec. Ther., vol. 6, 2002, pp.637-644.
Yacoub A., Mitchell C., Lister A. et al., “Melanoma differentiation-associated 7 (interleukin 24) inhibits growth and enhances radiosensitivity of glioma cells in vitro and in vivo,” Clin. CancerRes., vol.9, 2003, pp.3272 – 3281.
Makedonov GP, Tskhovrebova LV, Vasil'eva IM, Zasukhina GD.,“Radioadaptive response and antimutagenic effect of interferon have common pathways of cell protection against gamma radiation,”Dokl Akad Nauk, vol. 359, No. 6, 1998, pp. 838-40.(in Russian)
Jason J., Archibald L.K., Nwanyanwu O.C. et al., “Comparison of serum and cell-specific cytokines in humans,”Clin. Diag. Lab. Immunol., vol.8, No.6, 2001, pp.1097- 1103.
Fahey J.L., Aziz N., Spritzler J. et al., ”Need for external proficiency testing program for cytokines, chemokines, and plasma markers of immune activation,” Clin. Diag. Lab. Immunol., vol.7, No.4, 2000, pp.540-548.