Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation
American Journal of Life Sciences
Volume 3, Issue 1-2, January 2015, Pages: 18-24
Received: Nov. 24, 2014; Accepted: Nov. 26, 2014; Published: Dec. 27, 2014
Views 3627      Downloads 118
Authors
Margarita Vyalkina, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Anatoly Fedorov, Lab of the Fundamental Investigations in Ophthalmology, Research Institute of Eye Diseases, Moscow, Russia
Erika Eskina, Ophthalmological Department of Federal Medical-Biology Agency of Russia, Sphere Eye Clinic, Moscow, Russia
Yulia Medvedeva, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Elena Arkhipova, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Marina Lebedeva, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Nadezhda Khlebnikova, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Mikhail Baranov, Experimental Clinical Dept., Research Institute for Space Medicine, Federal Biomedical Agency of Russia, Moscow, Russia
Mikhail Karganov, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Article Tools
Follow on us
Abstract
Microgravity simulation in rats resulted to changes in eye lavage content. Molecular subfraction analysis with laser correlation spectroscopy revealed significant differences in the pattern of particle distribution in five intervals: 1.9 -2.56 nm, 4.64-6.25 nm, 91.3 nm, 165-300 nm, and 400-734 nm. Microgravity modeling induced a series of interrelated processes in the retina of experimental rats manifesting in decreasing of total and layer-by-layer thickness of the retina, mild edema, and consequently, the increasing of specific density of neurons. In the inner nuclear layer, the cell density decreased because of the amacrine and bipolar cells. These changes could be explained by relative ischemia of the retinal layers, which are dependent on the retinal vessels circulation system.
Keywords
Amacrine Cells, Bipolar Cells, Laser Correlation Spectroscopy, Microgravity Simulation, Muller Cells, Retinal Vessels
To cite this article
Margarita Vyalkina, Anatoly Fedorov, Erika Eskina, Yulia Medvedeva, Elena Arkhipova, Marina Lebedeva, Nadezhda Khlebnikova, Mikhail Baranov, Mikhail Karganov, Metabolic and Morphological Changes in Rats’ Eye after 7-Days Microgravity Simulation, American Journal of Life Sciences. Special Issue: Space Flight Factors: From Cell to Body. Vol. 3, No. 1-2, 2015, pp. 18-24. doi: 10.11648/j.ajls.s.2015030102.14
References
[1]
Zwart S.R., Gibson C.R., Mader T.H., Ericson K., Ploutz-Snyder R., Heer M., Smith S.M., “Vision changes after spaceflight are related to alterations in folate– and vitamin B-12–dependent one-carbon metabolism,” J. Nutr. 142: 2012, pp. 427–431.
[2]
Taibbi G., Kaplowitz K., Cromwell R.L., Godley B.F., Zanello S.B., Vizzeri G., “Effects of 30-Day Head-Down Bed Rest on Ocular Structures and Visual Function in a Healthy Subject,” Aviat Space Environ Med, vol. 84(2), 2013, pp. 148-154.
[3]
Zanello S.B., Nguyen A., Theriot C.A., “Retinal non-visual photoreception in space,” Aviat Space Environ Med, vol.84(12), 2013, pp. 1277-1280.
[4]
Chylack L.T. Jr, Peterson L.E., Feiveson A.H., Wear M.L., Manuel F.K., Tung W.H., Hardy D.S., Marak L.J., Cucinotta F.A., “NASA study of cataract in astronauts (NASCA). Report 1: Cross-sectional study of the relationship of exposure to space radiation and risk of lens opacity,” Radiat Res., vol.172(1), 2009, pp.10-20. doi: 10.1667/RR1580.1.
[5]
Roberts J.E., Kukielczak B.M., Chignell C.F., Sik B.H., Hu D.N., Principato M.A., “Simulated microgravity induced damage in human retinal pigment epithelial cells,” Mol Vis., vol.30(12), 2006, pp. 633-538.
[6]
Morey E.R., Sabelman E.E., Turner R.T., Baylink D.J., “A new rat model simulating some aspects of space flight,” Physiologist, vol.22, No. 6, 1979, pp.S23-24.
[7]
Ilin E.A., Novikov V.E., “Stand for modelling the physiological effects of weightlessness in laboratory experiments with rats,” Kosm Biol Aviakosm Med, vol.14, No 3, 1980, pp.79-80. (in Russian)
[8]
Karganov M., Alchinova I., Arkhipova E., Skalny A.V., “Laser Correlation Spectroscopy: Nutritional, Ecological and Toxic Aspects,” In: “Biophysics”. A.N. Misra ed.- InTech, 2012, -ISBN 978-953-51-0376-9. P.1-16. DOI: 10.5772/35254
[9]
Karganov M., Skalny A., Alchinova I., Khlebnikova N., Grabeklis A., Lakarova E., Eisazadeh S., “Combined use of laser correlation spectroscopy and ICP-AES, ICP-MS determination of macro- and trace elements in human biosubstrates for intoxication risk assessment,” Trace elements and electrolytes, vol. 28, No 2, 2011, pp.124-127.
[10]
B. E. Crucian, R. P. Stowe, D. L. Pierson, C. F. Sams, “Immune system dysregulation following short- vs longduration spaceflight,” Aviation Space and Environmental Medicine, vol. 79, No 9, 2008, pp. 835–843.
[11]
G. Sonnenfeld, J. S. Butel, W. T. Shearer, “Effects of the space flight environment on the immune system,” Reviews on Environmental Health, vol. 18, No. 1, 2003, pp. 1–17.
[12]
Girardi C., De Pittà C., Casara S., Calura E., Romualdi C., Celotti L., Mognato M., “Integration Analysis of MicroRNA and mRNA Expression Profiles in Human Peripheral Blood Lymphocytes Cultured in Modeled Microgravity,” BioMed Research International, 2014, Article ID 296747, 16 pages, http://dx.doi.org/10.1155/2014/296747
[13]
Alchinova I., Arkhipova E., Medvedeva Yu., Cherepov A., Antipov A., Lysenko N., Noskin L., Karganov M., “The complex of tests for the quantitative evaluation of the effects of radiation on laboratory animals,” – this issue, pp. 5 – 12.
[14]
N.N. Khlebnikova, D.L. Tarshits, M. Yu. Karganov, L.A.Noskin, Yu.S. Astashov, V.R. Grabovitsky, E. V.Botcharov, A. V. Bolshunov, P.I. Ganzovsky, M.N. Ivanov, “Application perspectives of plaintive fluid LCS method in diagnostics of pathological process gravity at conservative and operative methods of eye illnesses treatment,” Lazernaya meditsina, vol.3, No. 3-4, 1999, pp. 67-73 (in Russian)
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186