The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals
American Journal of Life Sciences
Volume 3, Issue 1-2, January 2015, Pages: 5-12
Received: Nov. 8, 2014;
Accepted: Nov. 12, 2014;
Published: Nov. 14, 2014
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Irina Alchinova, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Elena Arkhipova, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Yulia Medvedeva, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Anton Cherepov, Dept. of Experimental Biology and Cell Physiology, Research Institute for Space Medicine, Federal Biomedical Agency of Russia, Moscow, Russia; Lab of Neurobiology of Memory, P. K. Anokhin Institute of Normal Physiology, Moscow, Russia
Alexander Antipov, Veterinary Dept., K. I. Skryabin Moscow state Academy of veterinary medicine and biotechnology, Moscow, Russia
Nikolai Lysenko, Biology Dept., K. I. Skryabin Moscow state Academy of veterinary medicine and biotechnology, Moscow, Russia
Leonid Noskin, Rectorate, Moscow state pedagogical University, Moscow, Russia
Mikhail Karganov, Lab of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscow, Russia
Exposure to ionizing radiation leads to exhaustion of the pool of stem cells, increases differentiated cell stress, and activated reparation and apoptosis processes. Apart from well-documented acute effects, radiotherapy leads to delayed effects manifesting in few years after successful treatment. The aim of our study was the development of a test battery for evaluation of irradiation aftereffects in organisms characterized by different radiosensitivity. We chose C57BL mice as the ideal strain, because according to published data, they are characterized by medium radiosensitivity. At the preliminary stage, we have to assess informative value of some methods for the sublethal irradiation dose of 750 R. For evaluation of the damaging effects of radiation (lymphocyte count and maturity), we used, along with classical criteria, some complex parameters (body weight, serum homeostasis, behavior) characterizing general status of the organism. We also performed histological analysis of the liver, spleen, and pancreas and ranged the injuries by their severity.
The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals, American Journal of Life Sciences. Special Issue: Space Flight Factors: From Cell to Body.
Vol. 3, No. 1-2,
2015, pp. 5-12.
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