Please enter verification code
Special Issues
Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21
American Journal of Life Sciences
Volume 2, Issue 6-3, December 2014, Pages: 22-30
Received: Oct. 19, 2014; Accepted: Dec. 8, 2014; Published: Mar. 23, 2017
Views 2596      Downloads 78
Amin Jafari, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Jamshid Raheb, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Hassan Bardania, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Behnam Rasekh, Department of Petroleum Biotechnology, Biotechnology Research Center, Research Institute of Petroleum Industry, Tehran, Iran
Article Tools
Follow on us
The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.
MEOR, Manipulated E. coli BL21, Monorhamnolipid, Logarithmic Phase
To cite this article
Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh, Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21, American Journal of Life Sciences. Special Issue: Microbiology Research. Vol. 2, No. 6-3, 2014, pp. 22-30. doi: 10.11648/j.ajls.s.2014020603.15
Abalos, A., Pinazo, A., Infante, M. R. M., Casals, García, F. and Manresa, A., 2001. Physicochemical and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wast. Langmuir 17 (5): 1367-1371.
Abdel-Mawgoud, A. M., Lépine, F. and Déziel, E., 2010. Rhamnolipids: diversity of structures, microbial origins, and roles." Appllied Microbiology and Biotechnology 86(5): 1323-1336.
Al-Tahhan, R. A., Sandrin, T. R., Bodour, A. A. and Maier, R. M., 2000. Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa: effect on cell surface properties and interaction with hydrophobic substrates. Appllied Environmental Microbiology 66(8): 3262-3268.
Arino, S., Marchal, R. and Vandecasteele, J. P., 1998. Involvement of a rhamnolipid-producing strain of Pseudomonas aeruginosa in the degradation of polycyclic aromatic hydrocarbons by a bacterial community. Journal of Applied Microbiology 84(5): 769-776.
Banat, I. M., Makkar, R. S. and Cameotra, S. S., 2000. Potential commerical applications of microbial surfactants. Appllied Microbiology and Biotechnology 53(5): 495-508.
Bjarnsholt, T. a. G., M., 2007. The role of quorum sensing in the pathogenicity of the cunning aggressor Pseudomonas aeruginosa. Anal Bioanal Chem. 387(2): 409-414.
Caiazza, N. C., Shanks, R. M. and O'Toole, G. A., 2005. Rhamnolipids modulate swarming motility patterns of Pseudomonas aeruginosa. Journal of Bacteriology 187(21): 7351-7361.
Catherine, N. M., Raymond, N. Y. and Bernard, F. G., 2001. Heavy metal removal from sediments by biosurfactants. Journal of Hazardous Materials 85(1-2): 111-125.
Chayabutra, C., Wu, J. and Ju, L. K., 2001. Rhamnolipid production by Pseudomonas aeruginosa under denitrification: effects of limiting nutrients and carbon substrates. Biotechnology Bioengineering 72(1): 25-33.
Gallardo, M., Ferrandez, E. A., De Lorenzo, V., Garcia, J. L. and Diaz, E., 1997. Designing recombinant Pseudomonas strains to enhance biodesulfurization. Journal of Bacteriology 179(22): 7150-7160.
Gerardo, M., Katy, J., Brenda, V. and Gloria, S.C., 2003. Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. Journal of Bacteriology 185(20): 5976-5983.
Glick, R., Gilmour, C., Tremblay, J., Satanower, S., Avidan, O., Déziel, E., Greenberg, E. P., Poole, K. and Banin, E., 2010. Increase in rhamnolipid synthesis under iron-limiting conditions influences surface motility and biofilm formation in Pseudomonas aeruginos. Journal of Bacteriology 192(12): 2973-2980.
Haba, E., Pinazo, A., Jauregui, O., Espuny, M. J., Infante, M. R. and Manresa, A., 2003. Physicochemical characterization and antimicrobial properties of rhamnolipids produced by Pseudomonas aeruginosa 47T2 NCBIM 40044. Biotechnology and Bioengineering 81(3): 316-322.
Jiang, Z., L. H., Zhangy, H., Li, C., 2011. Oxidative Desulfurization of Fuel Oils Chinese Journal of Catalysis 32: 707–715.
15 Karanth, N. G. K., Deo, P. G. and Veenanadig, N. K., 1999. Microbial production of biosurfactants and their importance. Current Science 77(1): 116–123.
Koch, A. K., Käppeli, O., Fiechter, A. and Reiser, J., 1991. Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. Journal of Bacteriology 173(13): 4212-4219.
Kun, Z. and Charles, O. R., 2008. RhlA converts b-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the b-hydroxydecanoyl-b-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa. Journal of Bacteriology 190(9): 3147-3154.
Latifi, A., Foglino, M., Tanaka, K., Williams, P. and Lazdunski, A., 1996. A hierarchical quorum-sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary-phase sigma factor RpoS. Molecular Microbiology 21(6): 1137-1146.
Luis, H., Guerra S., Othmar K. and Armin F., 1996. Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors. Applied Microbiology and Biotechnology 24(6): 443-448.
Mazaheri-Assadi, M., Tabatabaee, M. S., (2010). Biosurfactants and their Use in Upgrading Petroleum Vacuum Distillation Residue: A Review. Int. J. Environ. Res. 4: 549-572.
Meyer, R. F. and Attanasi, E. D., 2003. Heavy Oil and Natural Bitumen – Strategic Petroleum Resources. U.S. Geological Survey, Fact Sheet 70-03.
Mulligan C. N. 2005. Environmental applications for biosurfactants. Environmental Pollution 133(2): 183-198.
Ochsner, U. A., Fiechter, A. and J. Reiser, 1994. Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeroginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis. Journal of Biological Chemistry 269: 19787_19795.
Ochsner, U. A., R., J., 1995. Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences (PNAS) 92(14): 6424-6428.
Pearson, J. P., Pesci, E. C. and Iglewski, B. H. 1997. Roles of Pseudomonas aeruginosa las and rhl quorumsensing systems in control of elastase and rhamnolipid biosynthesis genes. Journal of Bacteriology 179(18): 5756-5767.
Pesci, E. C., Pearson, J. P., Seed, P. C. and Iglewski, B. H. 1997. Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. Journal of Bacteriology 179(10): 127-3132.
Philp, J, C, T. Y., Guerinik, K., Al-Saleh, M, A., Sanders P, F., 2008. From Petroleum Microbiology to Biotechnology: Prospects for Saudi Aramco. Saudi Aramco Journal of Technology: 87-97.
Raheb, J, H. M., Saadati, M, Rasekh, B, Memari, B., 2009. The enhancement of biodesulfurization activity in a novel indigenous engineered Pseudomonas putida. Iran Biomed J. 13: 207-213.
Rosenberg, E., R. E. 1999. High- and low-molecular-mass microbial surfactants. Appl Microbiol Biotechnol 52: 154-162.
Singh, A, V. H. J. and Ward, O. P., 2007. Surfactants in microbiology and biotechnology: Part 2. Application aspects. Biotechnol Adv. 25: 99-121.
Teodoriu, C., F. G. and Espinel, A., 2007. Letting Off Steam and Getting Into Hot Water – Harnessing the Geothermal Energy Potential of Heavy Oil Reservoirs. 20th World Energy Congress–Rome.
Tremblay, J., Richardson, A. P., Lépine, F. and Déziel, E., 2007. Self-produced extracellular stimuli modulate the Pseudomonas aeruginosa swarming motility behaviour. Environmental Microbiology 9(10): 2622-2630.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186