Phytochemical and Antibacterial Screening of Crude Extracts from Leaves of Wonderful Kola
American Journal of Life Sciences
Volume 2, Issue 6-3, December 2014, Pages: 9-12
Received: Sep. 18, 2014;
Accepted: Sep. 25, 2014;
Published: Sep. 30, 2014
Views 3588 Downloads 265
Ejikeugwu Chika, Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, P. M. B 5025, Awka, Nigeria
Umeokoli Blessing, Department of Pharmaceutical and Medicinal Chemistry, Nnamdi Azikiwe University, P. M. B 5025, Awka, Nigeria
Iroha Ifeanyichukwu, Department of Applied Microbiology, Ebonyi State University, P.M.B 053, Abakaliki, Nigeria
Ugwu Malachy, Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, P. M. B 5025, Awka, Nigeria
Esimone Charles, Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, P. M. B 5025, Awka, Nigeria
Follow on us
The several antibacterial activities of wonderful kola plant (i.e. Buchholzia coriacea) has necessitated the need for a high-throughput and molecular characterization of all the bioactive components from the plant as a panacea to discovering bioactive compounds which could serve as lead compounds in developing potent and novel antibacterial agents. Microbial resistance to some available antimicrobial agents necessitates the need to search for newer antimicrobial agents that will not be easily amenable to the attack of resistant strains of microorganisms (many of which remain active even in the face of antimicrobial onslaught). This study investigates the phytochemical constituents and antibacterial activity of leaf extracts of wonderful kola on pathogenic Staphylococcus aureus strains. Antibacterial activity of the wonderful kola plant extract was evaluated by the agar well diffusion method to determine the zones of inhibition and minimum inhibitory concentration (MIC). Phytochemical analysis revealed that wonderful kola leaf contains phlobatannins, carbohydrates, proteins, tannins, saponins, alkaloids and flavonoids. Varying zones of inhibition was produced by the ethanol extract (10-14 mm) and methanol extracts (13-15 mm) against the S. aureus strains. MIC for both the ethanol and methanol extracts was recorded at 50 mg/ml and 100 mg/ml for some strains of the S. aureus. The results of this study have shown and contributed to the potential of wonderful kola leaf extracts in inhibiting pathogenic strains of S. aureus. Further high-throughput technologies are required to characterize the main bioactive constituents of the wonderful kola plant extracts so that they can be compounded into drug formulations for the treatment of some bacterial related infections in this part of the world.
Wonderful Kola, Gram Negative Bacteria, Resistance, Nigeria
To cite this article
Phytochemical and Antibacterial Screening of Crude Extracts from Leaves of Wonderful Kola, American Journal of Life Sciences. Special Issue:Microbiology Research.
Vol. 2, No. 6-3,
2014, pp. 9-12.
Esimone CO, Akah PA, Nworu CS. Efficacy and Safety Assessment of T. angelica Herbal Tonic, a Phytomedicinal product popularly used in Nigeria. Evidence – Based complementary and alternative Medicine 2009; 1-6.
Ezeja MI, Ezeigbo II and Madubuike KG. Analgesic activity of the methanolic seed extract of Buchholzia coriacea. Research Journal of Pharmaceutical, Biological and chemical Sciences 2011; 2:187-193.
Kareem KT, Kareem SO, Adeyemo OJ and Egberongbe RK. In vitro antimicrobial properties of Bridelia ferruginea on some clinical isolates. Agriculture and Biology Journal of North America 2010; 1(3):416-420.
Oluseyi EO, Onyeoziri NF. Preliminary studies on the antimicrobial properties of Buchholzia coriacea (wonderful kola). African Journal of Biotechnology 2009; 8(3):472-474.
Olayemi JO, Essien EE, Ajaiyeoba EO, Ekundayo O. Phytochemical and antimicrobial properties of Solanum macranthum Dunal. African Journal of Biotechnology 2011; 11(21) 4934-4937.
Mbata TI, Duru CM, Onwumelu HA. Antibacterial activity of crude seed extracts of Buchholzia coriacea E. on some pathogenic bacteria. Journal of Development Biology and Tissue Engineering 2009; 1(1):001-005.
Cheesbrough M. Biochemical tests to identify bacteria. In: District Laboratory Practice in Tropical Countries. 2nd edition., UK, Cambridge University Press 2000; 178- 187.
Ajayi IA, Ajibade O, Oderinde RA. Preliminary phytochemical analysis of some plant seeds. Research Journal of Chemical Sciences 2011; 1(3):58-62.
Akinyemi KO, Oladapo O, Okwara CE, Ibe CC and Fasure KA. Screening of crude extracts of six medicinal plants used in south-west Nigerian unorthodox medicine for anti methicillin-resistant Staphylococcus aureus activity. BMC Complementary and Alternative medicine 2005; 5(6):1-7.
Nweze NE. Studies on the antioxidant and antimicrobial activities of the seed extracts of Buchholzia coriacea (Capparaceae). Nigerian Veterinary Journal 2011 32(2):143-147.