Evaluation of Antibiotics Ampicillin and Ciprofloxacin and Biosurfactant Rhamnolipid Effect on Hydrophobicity and Electron Donors and Recipients in Urinary and Fecal Escherichia Coli
American Journal of Life Sciences
Volume 6, Issue 3, June 2018, Pages: 39-46
Received: Jul. 27, 2018; Accepted: Oct. 4, 2018; Published: Nov. 6, 2018
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Mahdi Khangholi, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Shaghayegh Anvari, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Ezzatollah Ghaemi, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Ailar Jamalli, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
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Adhesion is the first and most important stage in pathogenesis after bacteria enters to the body. Attachment of bacteria in medicine, industry, agriculture, waste decomposition, shipbuilding, etc. is important. Hydrophobicity and electron donor- electron acceptor characteristic are more important factors in bacterial adhesion. This study tries examining effect of biosurfactant rhamnolipid and two antibiotics ampicillin and ciprofloxacin on E. coli cell surface hydrophobicity and electron donor- electron acceptor characteristic by MATS method in terms of sample type and antibiotic resistance. Isolated bacteria from urine samples has a more antibiotic resistance to ampicillin. The results indicate that rhamnolipid makes increase in hydrophobicity and electron donor characteristic and in opposite ciprofloxacin makes increase electron acceptor and in opposite and decrease hydrophobicity. Also, hydrophobicity and electron donor- electron acceptor characteristic were different in sensitive and resistant to antibiotics strains. This study results showed since the hydrophobicity and electronic exchange are important factors involved in attachment of bacteria to inanimate surfaces and inner surfaces of the body, we can inhibit bacterial binding to it and help to reduce the incidence of antibiotic resistance by change these surfaces. The effect of antibiotics and rhamnolipid on some effective characteristic in adhesion cannot be ignored, despite the difference in their impact.
Adhesion, Hydrophobicity, Electron Donor-electron Acceptor Characteristic
To cite this article
Mahdi Khangholi, Shaghayegh Anvari, Ezzatollah Ghaemi, Ailar Jamalli, Evaluation of Antibiotics Ampicillin and Ciprofloxacin and Biosurfactant Rhamnolipid Effect on Hydrophobicity and Electron Donors and Recipients in Urinary and Fecal Escherichia Coli, American Journal of Life Sciences. Vol. 6, No. 3, 2018, pp. 39-46. doi: 10.11648/j.ajls.20180603.11
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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