Evaluation of Antibiotics Ampicillin and Ciprofloxacin and Biosurfactant Rhamnolipid Effect on Hydrophobicity and Electron Donors and Recipients in Urinary and Fecal Escherichia Coli
American Journal of Life Sciences
Volume 6, Issue 3, June 2018, Pages: 39-46
Received: Jul. 27, 2018;
Accepted: Oct. 4, 2018;
Published: Nov. 6, 2018
Views 774 Downloads 99
Mahdi Khangholi, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Shaghayegh Anvari, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Ezzatollah Ghaemi, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Ailar Jamalli, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
Adhesion is the first and most important stage in pathogenesis after bacteria enters to the body. Attachment of bacteria in medicine, industry, agriculture, waste decomposition, shipbuilding, etc. is important. Hydrophobicity and electron donor- electron acceptor characteristic are more important factors in bacterial adhesion. This study tries examining effect of biosurfactant rhamnolipid and two antibiotics ampicillin and ciprofloxacin on E. coli cell surface hydrophobicity and electron donor- electron acceptor characteristic by MATS method in terms of sample type and antibiotic resistance. Isolated bacteria from urine samples has a more antibiotic resistance to ampicillin. The results indicate that rhamnolipid makes increase in hydrophobicity and electron donor characteristic and in opposite ciprofloxacin makes increase electron acceptor and in opposite and decrease hydrophobicity. Also, hydrophobicity and electron donor- electron acceptor characteristic were different in sensitive and resistant to antibiotics strains. This study results showed since the hydrophobicity and electronic exchange are important factors involved in attachment of bacteria to inanimate surfaces and inner surfaces of the body, we can inhibit bacterial binding to it and help to reduce the incidence of antibiotic resistance by change these surfaces. The effect of antibiotics and rhamnolipid on some effective characteristic in adhesion cannot be ignored, despite the difference in their impact.
Evaluation of Antibiotics Ampicillin and Ciprofloxacin and Biosurfactant Rhamnolipid Effect on Hydrophobicity and Electron Donors and Recipients in Urinary and Fecal Escherichia Coli, American Journal of Life Sciences.
Vol. 6, No. 3,
2018, pp. 39-46.
Habimana O, Semião A, Casey E. The role of cell-surface interactions in bacterial initial adhesion and consequent biofilm formation on nanofiltration/reverse osmosis membranes. Journal of Membrane Science. 2014;454:82-96.
Wizemann TM, Adamou JE, Langermann S. Adhesins as targets for vaccine development. Emerging infectious diseases. 1999;5(3):395.
Bardiau M, Szalo M, Mainil JG. Initial adherence of EPEC, EHEC and VTEC to host cells. Veterinary research. 2010;41(5):57.
Hori K, Matsumoto S. Bacterial adhesion: From mechanism to control. Biochemical Engineering Journal. 2010;48(3):424-34.
Busscher HJ, van der Mei HC. How do bacteria know they are on a surface and regulate their response to an adhering state. Plos pathog. 2012;8(1):e1002440-e.
Tenke P, Kovacs B, Jäckel M, Nagy E. The role of biofilm infection in urology. World journal of urology. 2006;24(1):13-20.
Hsu LC, Fang J, Borca-Tasciuc DA, Worobo RW, Moraru CI. Effect of micro-and nanoscale topography on the adhesion of bacterial cells to solid surfaces. Applied and environmental microbiology. 2013;79(8):2703-12.
Krachler AM, Orth K. Targeting the bacteria–host interface: strategies in anti-adhesion therapy. Virulence. 2013;4(4):284-94.
Saralaya V, Bhat G, Kamath A, Shivananda P. Effect of trace elements on surface hydrophobicity and adherence of Escherichia coli to uroepithelial cells. Indian J Exp Biol. 2004;42:681-5.
Pizarro-Cerda J, Cossart P. Bacterial adhesion and entry into host cells. Cell. 2006;124(4):715-27.
Katsikogianni M, Missirlis Y. Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions. Eur Cell Mater. 2004;8(3).
Hamadi F, Latrache H, El Ghmari A, Ellouali M, Mabrrouki M, Kouider N. Effect of pH and ionic strength on hydrophobicity and electron donor and acceptor characteristics of Escherichia coli and Staphylococcus aureus. ANNALS OF MICROBIOLOGY. 2004;54:213-26.
Oliveira R, Azeredo J, Teixeira P, Fonseca A. The role of hydrophobicity in bacterial adhesion. 2001.
Gottenbos B, Grijpma DW, van der Mei HC, Feijen J, Busscher HJ. Antimicrobial effects of positively charged surfaces on adhering Gram-positive and Gram-negative bacteria. Journal of antimicrobial chemotherapy. 2001;48(1):7-13.
Burton E, Gawande PV, Yakandawala N, LoVetri K, Zhanel GG, Romeo T, et al. Antibiofilm activity of GlmU enzyme inhibitors against catheter-associated uropathogens. Antimicrobial agents and chemotherapy. 2006;50(5):1835-40.
Nataro JP, Kaper JB. Diarrheagenic escherichia coli. Clinical microbiology reviews. 1998;11(1):142-201.
Jacobsen S, Stickler D, Mobley H, Shirtliff M. Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis. Clinical microbiology reviews. 2008;21(1):26-59.
Galdiero S, Falanga A, Cantisani M, Tarallo R, Della Pepa ME, D’Oriano V, et al. Microbe-host interactions: structure and role of Gram-negative bacterial porins. Current protein & peptide science. 2012;13(8):843.
Al-Tahhan RA, Sandrin TR, Bodour AA, Maier RM. Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa: effect on cell surface properties and interaction with hydrophobic substrates. Applied and Environmental Microbiology. 2000;66(8):3262-8.
Zeraik AE, Nitschke M. Biosurfactants as agents to reduce adhesion of pathogenic bacteria to polystyrene surfaces: effect of temperature and hydrophobicity. Current microbiology. 2010;61(6):554-9.
Wojnicz D, Jankowski S. Effects of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to epithelial cells of uropathogenic Escherichia coli strains. International journal of antimicrobial agents. 2007;29(6):700-4.
Madani SH, Khazaee S, Kanani M, Shahi M. Antibiotic resistance pattern of E. coli isolated from urine culture in Imam Reza Hospital Kermanshah-2006. Journal of Kermanshah University of Medical Sciences (J Kermanshah Univ Med Sci). 2008;12(3).
Abdollahi Kheirabadi S, Najafipour S, Kafilzadeh F, Abdollahi A, Jafari S, Moravej A. Evaluation of Drug Resistance Pattern of Escherichia coli Strains Isolated from Fasa Vali-e-Asr Hospital Patients. Journal of Fasa University of Medical Sciences. 2013;2(4):273-8.
Kustos T, s a, Kustos I, oacute, Kilár F, Rappai G, et al. Effect of antibiotics on cell surface hydrophobicity of bacteria causing orthopedic wound infections. Chemotherapy. 2003;49(5):237-42.
Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols. 2008;3(2):163-75.
Sodagari M, Wang H, Newby B-mZ, Ju L-K. Effect of rhamnolipids on initial attachment of bacteria on glass and octadecyltrichlorosilane-modified glass. Colloids and Surfaces B: Biointerfaces. 2013;103:121-8.
Bellon-Fontaine M-N, Rault J, Van Oss C. Microbial adhesion to solvents: a novel method to determine the electron-donor/electron-acceptor or Lewis acid-base properties of microbial cells. Colloids and Surfaces B: Biointerfaces. 1996;7(1):47-53.
Baskin H, Doğan Y, Bahar IH, Yuluğ N. Effect of subminimal inhibitory concentrations of three fluoroquinolones on adherence of uropathogenic strains of Escherichia coli. International journal of antimicrobial agents. 2002;19(1):79-82.
Tahmourespour A, Kasra KR, SALEHI R, NABINEZHAD A. The relationship between cell surface hydrophobicity and antibiotic resistance of streptococcal strains isolated from dental plaque and caries. 2008.
Feng W, Swift S, Singhal N. Effects of surfactants on cell surface tension parameters and hydrophobicity of Pseudomonas putida 852 and Rhodococcus erythropolis 3586. Colloids and Surfaces B: Biointerfaces. 2013;105:43-50.
Rivas L, Fegan N, Dykes G. Physicochemical properties of Shiga toxigenic Escherichia coli. Journal of applied microbiology. 2005;99(4):716-27.
Djeribi R, Boucherit Z, Bouchloukh W, Zouaoui W, Latrache H, Hamadi F, et al. A study of pH effects on the bacterial surface physicochemical properties of Acinetobacter baumannii. Colloids and Surfaces B: Biointerfaces. 2013;102:540-5.