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Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase
American Journal of Life Sciences
Volume 4, Issue 2, April 2016, Pages: 47-50
Received: Feb. 5, 2016; Accepted: Feb. 27, 2016; Published: May 6, 2016
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David Morakinyo Sanni, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Oluwasegun Victor Omotoyinbo, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
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This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.
Datura metel, Tyrosinase, Inhibition
To cite this article
David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo, Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase, American Journal of Life Sciences. Vol. 4, No. 2, 2016, pp. 47-50. doi: 10.11648/j.ajls.20160402.15
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