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In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia
American Journal of Life Sciences
Volume 3, Issue 5, October 2015, Pages: 365-368
Received: Jul. 30, 2015; Accepted: Aug. 6, 2015; Published: Sep. 2, 2015
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Teklit Gebregiorgis Amabye, Department of Chemistry, College of Natural and Computational Science, Mekelle University, Mekelle, Ethiopia
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To examine the in vitro anti bacterial activities of the ethanol extract of fresh leaves of Alliumcepa (onion) and to determine and quantify the phenol compounds of the investigated plantparts. This study was drifting out at the Mekelle university department chemistry and Adigrat pharmaceutical industry, fromMarch2015toApril 2015. Clinical strains of Streptococcus pneumoniae and ethanol extracts of the plant species was used for the antimicrobial study. Thirty grams of the sample was ground, filtrated, and each filtrate mixed with 100 ml ethanol and placed in a shaker for 48 hours. Theethanol was evaporated from the sample, weighed, and subjected to an antibacterial activity test using the agar diffusion method. The high- performance liquid chromatography was used to identify and quantify the phenols extracts of investigated samples. Ethanol extract of the investigated plant parts showed antibacterial activities against different pathogenicbacteria. Leaf extracts of Alliumcepashowed the highest antibacterial activity and contains more phenols. Theethanol extract of the tested plants could be considered as analternative source of new antibacterialdrugs.
HPLC, Antimicrobial Empire, Streptococcus Pneumoniae, Medicinal Plants
To cite this article
Teklit Gebregiorgis Amabye, In Vitro Antimicrobial Efficacy of Fractions from Onion (Allium Cepa) Leaves Extract from Wukro, Ethiopia, American Journal of Life Sciences. Vol. 3, No. 5, 2015, pp. 365-368. doi: 10.11648/j.ajls.20150305.15
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