Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections
American Journal of Life Sciences
Volume 3, Issue 3, June 2015, Pages: 230-237
Received: May 8, 2015; Accepted: Jun. 1, 2015; Published: Jun. 14, 2015
Views 4174      Downloads 132
Hatil Hashim Elkamali, Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan
Sana Eltayeb Mahjoob, Department of Biochemistry, Faculty of Medical Laboratories Science, Omdurman Islamic University, Omdurman, Sudan
Article Tools
Follow on us
Punica granatum, Citrullus colocynthes, Curcuma long, Albizia lebbeckandAzardirachta indicaare used in traditional medical practice in Sudan for the treatment of gastrointestinal tract infections. The methanol and aqueous extracts from different plant parts (Peel of fruits, rhizome, fruits, leaves, and Gum) respectively at a concentration of 100 mg/ml, were evaluated against 20 clinical isolates (2 were Salmonella typhi,5 Proteus mirabilis,4 Escherichia coli,5 Pseudomonas aeruginosa,3 Staphylococcus aureus, one was Salmonella para typhi B) and 5 standard bacterial strains (Staphylococcusaureus ATCC 25923), Bacillus subtilis (NCTC 8236), Escherichia coli (ATCC 25922), Salmonella typhi (ATCC1319106) and Klebsiella pneumoniae (ATCC 35657) were tested for their antibacterial properties using the Agar Diffusion Technique in vitro. Of all plants methanolic and aqueous extracts of Punica granatum were the most active with clinical isolates and standard bacterial strains showed relatively high antibacterial activity against most of the tested microorganisms with the diameter of inhibition zones ranging between 14 and 24 mm, whereas the methanolic extract of Curcuma long showed high antibacterial activity againstProteus mirabilis clinical isolate (1Z = 20 mm). Most susceptible Gram-negative clinical Isolates bacteria were Escherichia coli and Proteusmirabilis. Most susceptible Gram negative standard bacteria were Bacillus subtilis (NCTC 8236) and Escherichia coli (ATCC 25922) and least susceptible Gram negative bacterium was Klebsiella pneumoniae (ATCC 35657).In Gram positive standard bacteria, most susceptible was S.aureus (ATCC 25923).Antibiotics was used as standards drug for antibacterial assay. The results obtained appeared to confirm the antibacterial potential of the plants investigated, and their usefulness in the treatment of gastrointestinal tract infections.
Antibacterialactivity, Medicinal Plants, Clinical Isolates and Standard Pathogenic Bacteria
To cite this article
Hatil Hashim Elkamali, Sana Eltayeb Mahjoob, Evaluation of Antibacterial Activity of Some Medicinal Plants Used in Sudanese Folk Medicine for Treatment of Gastrointestinal Tract Infections, American Journal of Life Sciences. Vol. 3, No. 3, 2015, pp. 230-237. doi: 10.11648/j.ajls.20150303.25
Abdallah, E. M.(2011).Plants: An Alternative Source for Antimicrobials. Journal of Applied Pharmaceutical Science.1(06):16-20.
Ahmad, I and Beg, A.Z.( 2001). Antimicrobial and Phytochemical Studies on 45 Indian Medicinal Plants against Multi-Drug and Resistant Human Pathogens. Journal of Ethnopharmacology.74:113-123.
Almas, K.(1999). The Antimicrobial Effects of Extracts of Azadirachta indica (Neem) and Salvadora persica (arak) Chewing Sticks. Indian Journal of Dental Research.10(1):18-19.
Chandarana, H., Baluja,Sh., Chanda, S. V.( 2005). Comparison of Anti- bacterial Activities of Selected Species of Zingiberaceae Family and Some Synthetic Compounds. Turk Journal of Biology.29:83-97.
Chattopadhyay, I., Biswas, K., Bandyopadhyay, U., Banerjee, R .K.(2004). Turmeric and Curcumin: Biological Actions and Medicinal Applications. Current Science. 87(1):44-53.
Cheesbrough, M.(1984). Culture Media. In: Medical Laboratory Manual for Tropical Countries. Tropical Health Technology and Butterworth-Heineman. Cambridge.3:60-9,407-28.
Chengaiah, B., Rao, K.M., ALagusundaram, M., Kumar, K.M., Chetty, C.M.(2010).Medical Importance of Natural Dyes. International Journal of Pharmacy and Technology Research.2(1):144-154.
Cruikshank, R. (1975). Medical Microbiology: A guide to Diagnosis and Control of Infection. 1th ed. Edinburgh and London: E and S Livingston Ltd;888.
Das, A, K., Mandal, S. C., Banerjee, S. K.,Sinha , S., Das, J., Saha, B.P., Pal, M .(1999).Studies on Antidiarrhoeal Activity of Punica granatum Seed Extract in Rats. Journal of Ethnopharmacology.68(3):205-208.
Devatkal, S. K., Jaiswal, P., Jha ,S.N., Bharadwaj, R., Viswas, K. N. (2013).Antibacterial Activity of Aqueous Extract of Pomegranate Peel against Pseudomonas stutzeri Isolated from Poultry Meat. Journal of Food Science and Technology.50(3):555-560.
Doss, A., Muhamed Mubarack, H., Dhanabalan, R.(2009). Antibacterial Activity of Tannins from Solanum trilobatum Linn. Leaves. Indian Journal of Science and Technology.2 (2):41-43.
Duman, A.D., Ozgen, M., Dayisoylu, K. S., Erbil, N., Durgac, C. (2009).Antimicrobial Activity of Six Pomegranate (Punica granatum L.) Varieties and Their Relation to Some of Their Pomological and Phytonutrient Characteristics.Molecules . 14 (10):1808-1817.
El-Mahmood ,A. M., Ogbonna, O. B., Raji, M.(2010).The Antibacterial Activity of Azadarichta indica (Neem) Seeds Extracts against Bacterial Pathogens Associated With Eye and Ear Infections. Journal of Medicinal Plants Research.4 (14):1414-1421.
Fenglin, H., Ruili,L., bao, H.,Liang, M. (2004). Free Radical Scavenging Activity of Extracts Prepared from Fresh Leaves of Selected Chinese Medicinal Plants. Fitoterapia. 75:14-23.
Helmy, W.A., Amer, H., EL-Shayeb ,N. M.( 2007).Biological and Anti-microbial Activities of Aqueous Extracts from Neem Tree (Azadirachta indica A. Juss., Meliaceae).Journal of Applied Sciences Research. 3(10): 1050-1055.
Khatibi, R. (2011).Effect of alcoholics extract on rat livers and antibacterial screening of Citrullus colocynthis.Journal of Horticulture and Forestry. 3(13): 386-391.
Khatibi, R and Teymorri, J.(2011).Anticandidal Screening and Antibacterial of Citrullus colocynthis in South East of Iran. Journal of Horticulture and Forestry. 3(13):392-398.
Kumar, D and Sidhu, P .(2011). The Antimicrobial Activity of Azardirachta indica, Glycyrrhiza glabra, Cinnamum zeylanicum, Syzygium aromaticum, Accacia nilotica on Streptococcus mutans and Enterococcus faecalis - An In vitro Study. Endodontology. 23 (1):18-25.
Lekshmi,N. C., Sowmia,N., Viveka, S., Brindha,J.R., Jeeva, S.(2012). The Inhibiting Effect of Azadirachta indica against Dental Pathogens. Asian Journal of Plant Science and Research. 2(1):6-10.
Maji, S., Dandapat,P., Ojha, D., Maity, C., Halder,S.K., Das Mohapatra,P.K., Pathak,T.K., Pati,B.R., Samanta, A., Mondal, K.C. (2010).In vitro Antimicrobial Potentialities of Different Solvent Extracts of Ethnomedical Plants against Clinically Isolated Human Pathogens.Journal of Phytology,2 (4):57-64.
Mathabe,M.C., Nikolova ,R.V., Lall. N., Nyazema, N.Z.(2006). Antibacterial Activities of Medicinal Plants used for the Treatment of Diarrhoea in Limpopo Province, South Africa. Journal of Ethnopharma- cology.105:286-293.
Memon, U., Brohi, A., Ahmed, S. W., Azhar I., Bano, H. (2003).Antibacterial Screening of Citrullus Colocynthis.Pakistan Journal of Pharmaceutical Sciences. 16 (1):1-6.
Mishra, S.S., Gothecha,V.K. , Sharma ,A. (2010). Albizia Lebbeck: A short Review. Journal of Herbal Medicine and Toxicology .4 (2):9-15.
Moorthy, K., Punitha, T., Vinodhini, R., Sureshkumar, B. T., Vijayalakshmi, P., Thajuddin, N.(2013).Antimicrobial Activity and Qualitative Phytochemical Analysis of Punica granatum Linn. (PERICARP). Journal of Medicinal Plants Research.7(9): 474-479.
Najafi, Sh., Sanadgol, N., Nejad, B. S., Beiragi, M. A., Sanadgol, E.(2010). Phytochemical Screening and Antibacterial Activity of Citrullus colocynthis (Linn.) Schrad against Staphylococcus aureus. Journal of Medicinal Plants Research. 4 (22):2321-2325.
Nascimento, G. F., Locatelli, J., Freitas, P. C., Silva, G. L. (2000). Antibacterial Activity of Plant Extracts and Phytochemicals on Antibiotic-Resistant Bacteria.Braz. Journal of Microbiology. 31:1-4.
Naz, Sh., Jabeen, S., Ilyas, S., Manzoor, F., Aslam, F., Ali, A. (2010).Antibacterial Activity of Curcuma longa Varieties against Different Strains of Bacteria.Pakistan Journal of Botany. 42 (1):455-462.
Naz, S., Siddiqi, R., Ahmad, S., Rasool, S.A., Sayeed, S. A. (2007).Antibacterial Activity Directed Isolation of Compounds from Punica granatum. Journal of Food Science. 72 (9):341–345.
Nwozo, S. O., Ajayi, I. A., Obadare M. ( 2010 ).Phytochemical Screening and Antimicrobial Activity of 10 Medicinal Seeds from Nigeria.Medicinal and Aromatic Plant Science and Biotechnology .Global Science Books, Ltd. Japan.73-75.
Okunomo, K. and Egho, E.O. (2010).Economic Importance of Some Underexploited Tree Species in Nigeria: Urgent Need for Separate Research Centers .Continental Journal. Biological Sciences .3:16 - 32.
Patel, J. D., Patel, D. K., Shrivastava, A., Kumar, V. (2008).Screening of Plant Extracts used in Traditional Antidiarrhoeal Medicines against Pathogenic Escherichia coli. Scientific World. 6 (6):63-67.
Paul, J. P.(2008).Studies on Antimicrobial Efficiency of Citrullus colocynthis (L.) Schrad: A Medicinal Plant. Ethnobotanical Leaflets.12:944-47.
Phatak ,A.A., Patankar R. D., Galgatte ,U. C., Paranjape ,S. Y., Deshpande, A. S., Pande ,A. K., Thombre ,R. (2011).Antimicrobial Activity of A poly-herbal Extract against Dental Micro Flora. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2(2):533- 539.
Ramanathan, T., Gurudeeban, S,. Satyavani, K. (2011). Local of Anesthetic Effect Citrullus colocynthis on Rana hexadactyla. Research Journal of Medicinal Plant. 5(3):338-342.
Shan, B., Cai, Y.Z., Brooks, J. D., Corke, H.(2007.)The In vitro Antibacterial Activity of Dietary Spice and Medicinal Herb Extracts. International Journal of Food Microbiology.117 (10):112-119.
Singh,A., Sharma,P.K., Garg,G.(2010).Natural Products as Preservatives. International Journal of Pharma and Bio Sciences. 1(4):601-612.
Singh,R., Chandra, R.,Bose,M.,Luthra, P.M.(2002).Antibacterial Activity of Curcuma longa Rhizome Extract on Pathogenic Bacteria. Current Science. 83 (6):737-741.
Solanki, R. (2010).Some Medicinal Plants with Antibacterial Activity. Pharmacie Globale. International Journal of Comprehensive Pharmacy (IJCP).4 (10):1-4.
Subrahmanyam, M., Hemmady, A and Pawar, S. G.(2001). Antibacterial Activity of Honey on Bacteria from Wounds. Annals of Burns and Fire Disasters . XIV-n.1
Sunilson, J.A.,Suraj,R., Rejiha, G., Anandarajagopal, K.,Kumari,A.V., Promwichit,P.(2009).In vitro AntimicrobialEvaluation of Zingiber officinale, Curcuma longa and Alpinia galangal Extracts as Natural Food Preservatives.American Journal of Food Technology.5:1-8.
Thakurta, P., Bhowmik, P., Mukherjee, S., Hajra, T. K., Patra, A., Bag, P. K.( 2007). Antibacterial, Antisecretory and Antihemorrhagic Activity of Azadirachta indica used to Treat Cholera and Diarrhea in India. Journal of Ethnopharmacology. 111(3): 607-612.
Uma , B., Prabhakar, S. K., Rajendran ,S., Sarayu, L.Y. (2009). Anti- microbial Activity of Albizzia Lebbeck Benth against Infectious Diarrhoea. The Internet Journal of Microbiology.7:1-5.
Venkataswamy, R., Doss, A., Mubarack, H.M., Sukumar, M. (2010).Phytochemical, HPTLC Finger Printing and Antibacterial Activity of Acacia nilotica (L.) Delile.Hygeia. Journal for Drugs and Medicines.2. (2):38-42.
Vijayasanthi,M., Kannan,V., Venkataswamy,R., Doss, A. (2012). Evaluation of the Antibacterial Potential of Various Solvent Extracts of Acacia nilotica linn.Leaves. Hygeia.J.D.Med. 4 (1):91-96.
Vijayanand ,S., and Hemapriya, J.(2011).In vitro Antibacterial Efficacy of Peel and Seed Extracts of Punica granatum L.against Selected Bacterial Strains. International Journal of Medicobiological Research.1(4):231-234.
Voravuthikunchai, S., Lortheeranuwat, A., Jeeju, W., Sririrak, T., Phongpaichit, S., Supawita, Th . (2004) .Effective Medicinal Plants against Enterohaemorrhagic Escherichia coli O157:H7.Journal of Ethnopharma- cology.94:49-54.
WHO Publication.(2001).WWW. WHO. Int / emc-documents/ antimicrobial-resistance/ docs/ EGlobal-strat. Pdf. 22-4-2003.
Yogisha, S and Raveesha, K. A. (2009). In-vitro Antibacterial Effect of Selected Medicinal Plant Extracts. Journal of Natural Products. 2:64-69.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186