Chitosan as Antimicrobial Agent and Fatty Acid Absorber in Smoked Skipjack Tuna Processed Using Coconut Shell
American Journal of Life Sciences
Volume 3, Issue 2, April 2015, Pages: 93-99
Received: Jan. 16, 2015;
Accepted: Jan. 23, 2015;
Published: Mar. 4, 2015
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Amos Killay, Biology Study Programm, Faculty of Mathematic and Sciences, Pattimura University, Ambon, Indonesia
Nurpudji A. Taslim, Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Suryani As’ad, Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Meta Mahendratta, Department of Agricultural Technology, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia
Background: Chitosan, a hydrophilic biopolymer industrially obtained by N-deacetylation of chitin, which allow for a wide scope of applications and can be applied as an antimicrobial agent.Theaim of this study was to determine theconcentration of chitosan compound in which it can play an active role in inhibiting the growth of bacteria and also absorb the fatty acid /cholesterol in the smoked fish meat. Method: The research method used in this study wastheRandomized Block Design (RBD) factorial. There were two treatments in this study, namely: Treatment A (fish was dipped in a chitosan solution and then smoked), Treatment B (fish was smoked and then dipped in a 1%, 2% and 3% chitosan solution) and one control, in which the fish was smoked without being dipped in chitosan solution and then stored for five days. Results: The results showed that the chitosan concentration (1%, 2%, 3%) significantly affected the growth of bacteria that occurred on day 3 (p = 0,00) and day 5 (p = 0,000), while day 1 did not differ significantly. There was a difference in the levels of fatty acids between the control and A (p = 0,00) and the control with B (p = 0,000), with the best concentration of chitosan was 3%. Conclusion: Fifteen saturated fatty acids and eleven unsaturated fatty acids were found in fish smoked with coconut shells.
Nurpudji A. Taslim,
Chitosan as Antimicrobial Agent and Fatty Acid Absorber in Smoked Skipjack Tuna Processed Using Coconut Shell, American Journal of Life Sciences.
Vol. 3, No. 2,
2015, pp. 93-99.
Anoniem, 2008. Pood Microbiologie Testen. POM Republiek Indonesië Vol. 9, No.2.
Anonimous, 2008. Pengujian Mikrobiologi Pangan. Badan POM Republik Indonesia Vol. 9, No. 2.
Hui Liu, Yumin Du, Xiaohui Wang, Liping Sun, Chitosan kills bacteria through cell membrane damage. International Journal of Food Microbiology. 2004, 95; 147– 155.
Kim S.K., Rajapakse N., Enzymatic production and biological activities of chitosan oligosaccharides (COS): A review. Carbohydr Polym. 2005, 62(4):357-368.
Coma V., Deschamps A., Martial-Gros A., Bioactive packaging material from edible chitosan polymer-antimicrobial activity assessment on dairy related contamination. Journal of food science. 2003, 66(9): 2788-2792.
Dutta, P.K., Tripathi, P., Mehrotra, G.K., Dutta, J. Perspectives for chitosan based antimicrobial film in food applications. Food chemistry 2009, 114(4):1173-1182.
Nuasaen S, Opaprakasit P, Tangboriboonrat P. Hollow latex particles functionalized with chitosan for the removal of formaldehyde from indoor air. Carbohydr Polym. 2013; 101: 179-87. doi: 10.1016/j.carbpol.2013.09.059.
Wang N, Wang X, Jia Y, Li X, Yu J, Ding B. Electrospunnano fibrous chitosan membranes modified with polyethyleneimine for formaldehyde detection. Carbohydr. Polym. 2014; 108:192-9. Doi: 10.1016/j.carbpol.2014.02.088.
Natthan Charernsriwilaiwat, Praneet Opanasopit, Theerasak Rojanarata and Tanasait Ngawhirunpat, In Vitro Antioxidant Activity of Chitosan Aqueous Solution: Effect of Salt Form. Tropical Journal of Pharmaceutical Research April 2012, 11 (2): 235-242.
Wanvimol Pasanphan, Garry R. Buettner Suwabun Chirachanchai, Chitosangallate as a novel potential polysaccharide antioxidant: an EPR study. Carbohydrate Research. 2010, 132–140.
Hargono, Abdullah andIndro Sumantri. Production of Chitosan from Shrimp Waste and its Application to Lower Goat Fat Cholesterol (In Indoensia). Reaktor Journal, 2008, 12(1): 53-57.
Slamet Budijanto, Rokhani Hasbullah, Sulusi Prabawati, Setiadjit, Sukarno, Ita Zuraida. The Study of Coconut Shell Liquid Smoke Safety for Food Products (in Indonesia). Indonesian Agriculture Journal 2008, 13(3) : 236-240.
Entsar I. Rabea, Mohamed E.-T. Badawy, Christian V. Stevens, Guy Smagghe, and Walter Steurbaut. Chitosan as Antimicrobial Agent: Applications and Mode of Action. Biomacromolecules, 2003, 4 (6), pp 1457–1465.
A. S. Osheba, M. A. Sorour, Entsar, S. Abdou, Effect Of Chitosan Nanoparticles As Active Coating On Chemical Quality And Oil Uptake Of Fish Fingers. Journal of Agriculture and Environmental Science. June 2013, 2(1); 01-14.
Jain NK, Jain SK. Development and In Vitro Characterization of Galactosylated Low Molecular Weight Chitosan Nanoparticles Bearing Doxorubicin. AAPS Pharm Sci Tech. 2010, 11(2): 686-697. doi:10.1208/s12249-010-9422.
Raafat, D. Sahl H. Chitosan and its antimicrobial potential – a critical literature survey. Microbial biotechnology 2009, 2(2):186-201. doi:10.1111/j.1751-7915.2008.00080.
Dai T, Tanaka M, Huang Y-Y, Hamblin MR. Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects. Expert review of anti-infective therapy 2011, 9(7): 857-879. doi:10.1586/eri.11.59.
Quehenberger O, Armando IS, Dennis EA. High Sensitivity Quantitative Lipidomics Analysis of Fatty Acids in Biological Samples by Gas Chromatography-Mass Spectrometry. Biochimicaet biophysicaacta 2011, 1811(11): 648-656. doi:10.1016/j.bbalip.2011.07.006.