Molecular Mechanism of Formalin-Induced Toxicity and Its Management
American Journal of Life Sciences
Volume 3, Issue 2, April 2015, Pages: 85-92
Received: Feb. 14, 2015; Accepted: Feb. 25, 2015; Published: Mar. 3, 2015
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Authors
Alpana Khatun, Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh
Md Masud Rana, Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh
Md Rafiqul Islam Khan, Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh
Mir Imam Ibne Wahed, Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh
Md. Anwar Habib, Department of Pharmacology and Therapeutics, Rajshahi Medical College, Rajshahi-6000, Bangladesh
Md. Nazim Uddin, Department of Pharmacology and Therapeutics, Rajshahi Medical College, Rajshahi-6000, Bangladesh
Zakia Sultana Sathi, Department of Pharmacy, Daffodil International University, Dhaka 1207, Bangladesh
A. R. M. Ruhul Amin, Department of Hematology & Medical Oncology, Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA
Abu Syed Md Anisuzzaman, Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh; Department of Hematology & Medical Oncology, Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA
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Abstract
The use of formalin (40% formaldehyde) for the preservation of food in an illegal way becoming a serious health issue in developing countries including Bangladesh. We investigated the Formalin (FA)-induced organ toxicity in Swiss albino mice. FA induction caused the significant elevation of the liver enzyme, SGOT and SGPT; the MDA levels in the liver and brain. Among the fractions of methanol extract of L. globosus, ethyl acetate (EA) fraction significantly reduced the elevated biochemical parameters (FA vs FA + EA fraction, μKa/L); SGOT (78.4 ± 0.3 vs 14.3 ± 0.9), SGPT (100.5 ± 5.2 vs 14.6 ± 0.7), MDA in liver (10.9 ± 0.2 vs 5.6 ± 0.1) and MDA in brain (16.9 ± 0.2 vs 6.3 ± 0.2). Morphological analyses also supported the beneficial effect of EA fraction in FA-induced liver toxicity. FA induction caused the phosphorylation of JNK, member of mitogen activated protein kinase (MAPK) in both the liver and brain, which were completely abolished by the treatment of EA fraction of L. globosus. Chemical analyses showed that the EA fraction exhibited antioxidant and free radical scavenging properties. The protective effect of the EA fraction on the FA-induced toxicity by the modulation of oxidative inflammatory pathway by its antioxidant and free radical scavenging activity.
Keywords
Loranthus globosus, SGOT and SGPT, Antioxidant and Free Radical Scavenging, JNK Phosphorylation, Formalin
To cite this article
Alpana Khatun, Md Masud Rana, Md Rafiqul Islam Khan, Mir Imam Ibne Wahed, Md. Anwar Habib, Md. Nazim Uddin, Zakia Sultana Sathi, A. R. M. Ruhul Amin, Abu Syed Md Anisuzzaman, Molecular Mechanism of Formalin-Induced Toxicity and Its Management, American Journal of Life Sciences. Vol. 3, No. 2, 2015, pp. 85-92. doi: 10.11648/j.ajls.20150302.15
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