Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants
American Journal of Life Sciences
Volume 3, Issue 1, February 2015, Pages: 47-55
Received: Dec. 31, 2014;
Accepted: Jan. 18, 2015;
Published: Feb. 13, 2015
Views 2346 Downloads 147
Mohamed Maha Helmy, Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Gado Emad, Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia
El-Deeb Samir, Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Mostafa Helmy Mostafa, Plant Pathology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.
Mohamed Maha Helmy,
Mostafa Helmy Mostafa,
Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants, American Journal of Life Sciences.
Vol. 3, No. 1,
2015, pp. 47-55.
Burpee, L. L.; Sanders, P. L.; Cole, H. Jr. and Kim, S. H. (1978). A staining technique for nuclei of Rhizoctonia solani and related fungi. Mycologia 70: 1281-1283.
Carling, D.E. and Kuninaga, S. (1990). DNA based-sequence homology in Rhizoctonia solani Kühn: inter-and intra-group relatedness of anastomosis group-9. Phytopathology 80:1362-1364.
Elwakil, M. A.; El-Refai, I. M.; Awadallah, O. A.; El-Metwally, M. A. and Mohamed, M. S. (2009). Seed-borne pathogens of faba bean in Egypt: Detection and Pathogenicity. Plant Pathology Journal, 8(3): 90-97.
Fenille, R. C.; Ciampi, M. B.; Kuramae, E. E. and Souza, N. L. (2003). Identification of Rhizoctonia solani Associated with Soybean in Brazil by rDNA-ITS Sequences. Fitopatologia Brasileira 28:413-419. 2003.
Gonzalez, D.; Carling, D. E.; Kuninaga, S.; Vilgalys, R. And Cubeta, M. A. (2001). Rhibosomal DNA systematics of Ceratobasidium and Thanatephorus with Rhizoctonia anamorphs. Mycologia., 93: 1138-1150.
Kim, H. T.; Chung, Y. R. and Cho, K. Y. (2001). Mycelial melanization of Rhizoctonia solani AG1 affected pathogenicity in rice. Plant Patho. J., 17 (4): 210-215.
Kuninaga, S. and Yokosawa, R. (1982a). DNA based sequence homology in Rhizoctonia solani Kühn. I. Genetic relatedness within anastomosis group 1. Annals of the Phytopathological Society of Japan 48:659-667.
Kuninaga, S. and Yokosawa, R. (1982b). DNA based sequence homology in Rhizoctonia solani Kühn. II. Genetic relatedness within anastomosis group 2. Annals of the Phytopathological Society of Japan 48:668-673.
Kuninaga, S. and Yokosawa, R. (1984). DNA base-sequence homology in Rhizoctonia solani Kühn. IV. Genetic relatedness within AG-4. Ann Phytopathol Soc 50:322-330.
Kuninaga, S. and Yokosawa, R. (1985). DNA base sequence homology in Rhizoctonia solani Kühn. VI. Genetic relatedness among seven anastomosis groups. Ann. Phytopath. Soc. Japan. 51: 127-132.
Kuninaga, S; Natsuaki, T.; Takeuchi, T. and Yokosawa, R. (1997). Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani. Current Genetics., 32: 237-243.
Kuramae-Izioka, E.E. (1997). A rapid. easy and high yield protocol for total genomic DNA isolation from Colletotrichum gloeosporioides and Fusarium oxysporum for RAPD. Revista Unimar 19:683-689.
Lamari, L. and Bernier, C. C. (1985). Etiology of seedling blight and root rot of faba bean (Vicia faba) in Manitoba. Can. J. Plant Pathol., 7: 139-145.
Maha, H. Mohamed; Gado, E. A. M.; El-Deeb, S. H. and Mostafa, M. H. (2014). Behavior of fungus Rhizoctonia solani under faba bean cotyledons when away from host and the effect of its starvation on aggressiveness. Journal of Yeast and Fungal Research 5(1): 1-8.
Omar, S. A. M. (1986). Pathological studies on root rot diseases of faba bean (Vicia faba). Faba Bean Information Service (FABIS) Newsletter., 14: 34-37.
Parmeter, J. R. Jr.; Snyder, W. C. and Reichle, R. E. (1963). Heterokaryosis and variability in plant pathogenic fungi. Annual Review of Phytopathology, 1: 51-76.
Saitou,N., and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 (4): 406-425.
Salt, G. A. (1982). Factors affecting resistance to root rot and wilt diseaes. Pages 259-267 in G. C. Hawtin and C. Webb, eds., Faba Bean Improvement. Martinus Nijhoff Publishers, The Netherlands.
Sneh, B.; Burpee, L. and Ogoshi, A. (1991). Identification of Rhizoctonia Species. St Paul, MN, APS Press, pp. 133.
Thompson, J.D.; Gibson, T.J.; Plewniak, F.; Jeanmougin, F. and Higgins, D.G. (1997). The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876-4882.
Vilgalys, R. (1988). Genetic relatedness among anastomosis groups in Rhizoctonia as measured by DNA/DNA hybridization. Phytopathology 78:698-702.
White, T. J.; Bruns, T.; Lee, S. and Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J. (Eds.) PCR Protocols: A Guide to Methods and Applications. San Diego. Academic Press. pp. Pp. 315-322