Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease
American Journal of Life Sciences
Volume 2, Issue 6, December 2014, Pages: 400-405
Received: Dec. 15, 2014;
Accepted: Dec. 23, 2014;
Published: Jan. 6, 2015
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Olfat Mohamed Gab Allah, Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
Mona Assem Abd-Elmotelb, Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
Reda Gaber Saleh, Oral Biology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt
Recent breakthrough studies gave a considerable interest on the pathogenesis of periodontal diseases, particularly, the critical role of cytokines. Cytokines have dual roles; they could be mediators of host defense against the microbial challenge, or mediators for periodontal tissue alteration. Osteopontin (OPN) is a glycosylated phosphoprotein, it functions as a Th 1 (T – helper 1) cytokine, promotes cell-mediated immune responses and plays a role in chronic inflammatory diseases. To assess OPN expression in dentogingival junction (DGJ) in chronic periodontitis, ten 13–16 months old, beagle dogs were used. Animals were distributed randomly into two equal groups: Group I: animals had healthy periodontium. Group II: chronic periodontitis was induced in animals. Four weeks after induction, dogs were sacrificed and specimens were prepared for immunohistochemical staining using OPN monoclonal antibody. Intensity of OPN reactions was recorded by image J and t-test was used to compare between the two groups. Healthy periodontium showed a mild OPN reaction. While, in chronic periodontitis, moderate to intense extracellular reaction was seen in DGJ. Also, sporadic nuclear OPN reactions of inflammatory cells were noticed in chronic periodontitis. T-test revealed a significant difference between normal and chronic periodontitis groups. In conclusion, OPN may play a role in chronic inflammation and can be considered as a biomarker of periodontal disease progression.
Olfat Mohamed Gab Allah,
Mona Assem Abd-Elmotelb,
Reda Gaber Saleh,
Expression of Bone Matrix Protein (Osteopontin) in Dentogingival Junction in Periodontal Disease, American Journal of Life Sciences.
Vol. 2, No. 6,
2014, pp. 400-405.
Nanci, A. and D.D. Bosshardt, Structure of periodontal tissues in health and disease. Periodontol 2000, 2006. 40: p. 11-28.
Tsukamoto, Y., M. Usui, G. Yamamoto, Y. Takagi, T. Tachikawa, M. Yamamoto, and M. Nakamura, Role of the junctional epithelium in periodontal innate defense and homeostasis. J Periodontal Res, 2012. 47(6): p. 750-7.
Savage, A., K.A. Eaton, D.R. Moles, and I. Needleman, A systematic review of definitions of periodontitis and methods that have been used to identify this disease. J Clin Periodontol, 2009. 36(6): p. 458-67.
Grigoriadou, M.E., S.O. Koutayas, P.N. Madianos, and J.R. Strub, Interleukin-1 as a genetic marker for periodontitis: review of the literature. Quintessence Int, 2010. 41(6): p. 517-25.
Seguier, S., G. Godeau, and N. Brousse, Collagen fibers and inflammatory cells in healthy and diseased human gingival tissues: a comparative and quantitative study by immunohistochemistry and automated image analysis. J Periodontol, 2000. 71(7): p. 1079-85.
Seguier, S., G. Godeau, and N. Brousse, Immunohistological and morphometric analysis of intra-epithelial lymphocytes and Langerhans cells in healthy and diseased human gingival tissues. Arch Oral Biol, 2000. 45(6): p. 441-52.
O'Brien, E.R., M.R. Garvin, D.K. Stewart, T. Hinohara, J.B. Simpson, S.M. Schwartz, and C.M. Giachelli, Osteopontin is synthesized by macrophage, smooth muscle, and endothelial cells in primary and restenotic human coronary atherosclerotic plaques. Arterioscler Thromb, 1994. 14(10): p. 1648-56.
Thorat, M., P. Ar, and G. Garg, Correlation of Levels of Oncostatin M Cytokine in Crevicular Fluid and Serum in Periodontal Disease. International Journal of Oral Science, 2010. 2(4): p. 198-207.
Malyankar, U.M., M. Almeida, R.J. Johnson, R.H. Pichler, and C.M. Giachelli, Osteopontin regulation in cultured rat renal epithelial cells. Kidney Int, 1997. 51(6): p. 1766-73.
Devoll, R.E., W. Li, K.V. Woods, G.J. Pinero, W.T. Butler, M.C. Farach-Carson, and R.P. Happonen, Osteopontin (OPN) distribution in premalignant and malignant lesions of oral epithelium and expression in cell lines derived from squamous cell carcinoma of the oral cavity. J Oral Pathol Med, 1999. 28(3): p. 97-101.
Denhardt, D.T., C.M. Giachelli, and S.R. Rittling, Role of osteopontin in cellular signaling and toxicant injury. Annu Rev Pharmacol Toxicol, 2001. 41: p. 723-49.
Denhardt, D.T. and M. Noda, Osteopontin expression and function: role in bone remodeling. J Cell Biochem Suppl, 1998. 30-31: p. 92-102.
Knight, A., ed. The Costs and Benefits of Animal Experiments. May 2011, Palgrave Macmillan.272.
Nociti, F.H., Jr., R. Cesco De Toledo, M.A. Machado, C.M. Stefani, S.R. Line, and R.B. Goncalves, Clinical and microbiological evaluation of ligature-induced peri-implantitis and periodontitis in dogs. Clin Oral Implants Res, 2001. 12(4): p. 295-300.
Patarca, R., G.J. Freeman, R.P. Singh, F.Y. Wei, T. Durfee, F. Blattner, D.C. Regnier, C.A. Kozak, B.A. Mock, H.C. Morse, 3rd, and et al., Structural and functional studies of the early T lymphocyte activation 1 (Eta-1) gene. Definition of a novel T cell-dependent response associated with genetic resistance to bacterial infection. J Exp Med, 1989. 170(1): p. 145-61.
Slots, J. and M.A. Listgarten, Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontol, 1988. 15(2): p. 85-93.
Socransky, S.S., A.D. Haffajee, J.L. Dzink, and J.D. Hillman, Associations between microbial species in subgingival plaque samples. Oral Microbiology and Immunology, 1988. 3(1): p. 1-7.
Ramaiah, S.K. and S. Rittling, Pathophysiological role of osteopontin in hepatic inflammation, toxicity, and cancer. Toxicol Sci, 2008. 103(1): p. 4-13.
Takahashi, F., K. Takahashi, T. Okazaki, K. Maeda, H. Ienaga, M. Maeda, S. Kon, T. Uede, and Y. Fukuchi, Role of osteopontin in the pathogenesis of bleomycin-induced pulmonary fibrosis. Am J Respir Cell Mol Biol, 2001. 24(3): p. 264-71.
Xie, Y., M. Sakatsume, S. Nishi, I. Narita, M. Arakawa, and F. Gejyo, Expression, roles, receptors, and regulation of osteopontin in the kidney. Kidney Int, 2001. 60(5): p. 1645-57.
Weber, G.F., S. Zawaideh, S. Hikita, V.A. Kumar, H. Cantor, and S. Ashkar, Phosphorylation-dependent interaction of osteopontin with its receptors regulates macrophage migration and activation. J Leukoc Biol, 2002. 72(4): p. 752-61.
Pardo, A., K. Gibson, J. Cisneros, T.J. Richards, Y. Yang, C. Becerril, S. Yousem, I. Herrera, V. Ruiz, M. Selman, and N. Kaminski, Up-regulation and profibrotic role of osteopontin in human idiopathic pulmonary fibrosis. PLoS Med, 2005. 2(9): p. e251.
Bruemmer, D., A.R. Collins, G. Noh, W. Wang, M. Territo, S. Arias-Magallona, M.C. Fishbein, F. Blaschke, U. Kintscher, K. Graf, R.E. Law, and W.A. Hsueh, Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice. J Clin Invest, 2003. 112(9): p. 1318-31.
Olfat M. Gab Allah, H.M.E.T., Mona A. Abd-Elmotelb, Hoda M. El-Guindy , Reda G. Saleh, Nanotechnology and its Role in the Treatment of Induced Periodontitis (Experimental Study). rnal of American Scienc, 2014. 10(10): p. 179-195.
Kim, S.E., E.R. Lee, Y. Lee, M. Jeong, Y.W. Park, J.S. Ahn, J.T. Ahn, and K. Seo, A modified method for inducing periodontitis in dogs using a silk-wire twisted ligature. Journal of Veterinary Science, 2012. 13(2): p. 193-197.
Ashkar, S., G.F. Weber, V. Panoutsakopoulou, M.E. Sanchirico, M. Jansson, S. Zawaideh, S.R. Rittling, D.T. Denhardt, M.J. Glimcher, and H. Cantor, Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. Science, 2000. 287(5454): p. 860-4.
Yu, X.Q., D.J. Nikolic-Paterson, W. Mu, C.M. Giachelli, R.C. Atkins, R.J. Johnson, and H.Y. Lan, A functional role for osteopontin in experimental crescentic glomerulonephritis in the rat. Proc Assoc Am Physicians, 1998. 110(1): p. 50-64.
Tsai, A.T., J. Rice, M. Scatena, L. Liaw, B.D. Ratner, and C.M. Giachelli, The role of osteopontin in foreign body giant cell formation. Biomaterials, 2005. 26(29): p. 5835-43.
Sharma, C.G. and A.R. Pradeep, Plasma and crevicular fluid osteopontin levels in periodontal health and disease. J Periodontal Res, 2007. 42(5): p. 450-5.