The Effect of Aqueous Extract of Allium Sativum (Garlic) on Erythrocyte Osmotic Fragility in Normal and Sickle Cell
American Journal of Life Sciences
Volume 2, Issue 5, October 2014, Pages: 278-281
Received: Sep. 28, 2014; Accepted: Oct. 15, 2014; Published: Oct. 30, 2014
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Authors
Salami Hamza Adegoke, Department of Human Physiology, College of Medical Sciences, University of Maiduguri. Borno State, Nigeria
Gadaka Madu Adamu, Department of Biochemistry, Faculty of Science, University of Maiduguri. Borno State. Nigeria
John Adeolu Ige, Department of Human Physiology, College of Medical Sciences, University of Maiduguri. Borno State, Nigeria
Babagana Fatima, Department of Biochemistry, Faculty of Science, University of Maiduguri. Borno State. Nigeria
Odirachukwu Rita, Department of Biochemistry, Faculty of Science, University of Maiduguri. Borno State. Nigeria
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Abstract
Allium sativa (garlic) is a bulbous herb used as food item, spice and medicine in different parts of the world. The effect of garlic (Allium Sativa) on the osmotic fragility of red blood cells in normal and sickle cell patients was assessed in vitro. The study comprised four sets of fourteen centrifuge tubes each containing 5ml of graduated concentrations of normal saline. To the first set of tubes, 20μl of blood from normal subjects was added using a micropipette, while to the second, 0.75mg of garlic and 20μl of blood from normal subjects were added. To the third set of tubes, 20μl of blood from sickle cell patient was added, and to the fourth 20μl of blood from sickle cell patient and 0.75mg of garlic were added. Percentage hemolysis was determined by spectrophotometry. It was observed that the degree of hemolysis was greater (P<0.05) in the sickle cell compared to the normal blood. This observation was also recorded in the samples treated with garlic. It is concluded that the degree of hemolysis in sickle cell is higher (P<0.05) than normal cell. Treatment with garlic increased fragility in both normal and sickle cell blood however this effect was more pronounced in sickle cell blood. It is recommended that the consumption of garlic in patients with sickle cell anemia should be continuously monitored and carefully regulated.
Keywords
Allium Sativa (Garlic), Osmotic Fragility, Red Blood Cells, Membrane Stability, Sickle Cell Anemia, % Hemolysis
To cite this article
Salami Hamza Adegoke, Gadaka Madu Adamu, John Adeolu Ige, Babagana Fatima, Odirachukwu Rita, The Effect of Aqueous Extract of Allium Sativum (Garlic) on Erythrocyte Osmotic Fragility in Normal and Sickle Cell, American Journal of Life Sciences. Vol. 2, No. 5, 2014, pp. 278-281. doi: 10.11648/j.ajls.20140205.15
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