The Effect of Aqueous Extract of Allium Sativum (Garlic) on Erythrocyte Osmotic Fragility in Normal and Sickle Cell
American Journal of Life Sciences
Volume 2, Issue 5, October 2014, Pages: 278-281
Received: Sep. 28, 2014;
Accepted: Oct. 15, 2014;
Published: Oct. 30, 2014
Views 2552 Downloads 169
Salami Hamza Adegoke, Department of Human Physiology, College of Medical Sciences, University of Maiduguri. Borno State, Nigeria
Gadaka Madu Adamu, Department of Biochemistry, Faculty of Science, University of Maiduguri. Borno State. Nigeria
John Adeolu Ige, Department of Human Physiology, College of Medical Sciences, University of Maiduguri. Borno State, Nigeria
Babagana Fatima, Department of Biochemistry, Faculty of Science, University of Maiduguri. Borno State. Nigeria
Odirachukwu Rita, Department of Biochemistry, Faculty of Science, University of Maiduguri. Borno State. Nigeria
Follow on us
Allium sativa (garlic) is a bulbous herb used as food item, spice and medicine in different parts of the world. The effect of garlic (Allium Sativa) on the osmotic fragility of red blood cells in normal and sickle cell patients was assessed in vitro. The study comprised four sets of fourteen centrifuge tubes each containing 5ml of graduated concentrations of normal saline. To the first set of tubes, 20μl of blood from normal subjects was added using a micropipette, while to the second, 0.75mg of garlic and 20μl of blood from normal subjects were added. To the third set of tubes, 20μl of blood from sickle cell patient was added, and to the fourth 20μl of blood from sickle cell patient and 0.75mg of garlic were added. Percentage hemolysis was determined by spectrophotometry. It was observed that the degree of hemolysis was greater (P<0.05) in the sickle cell compared to the normal blood. This observation was also recorded in the samples treated with garlic. It is concluded that the degree of hemolysis in sickle cell is higher (P<0.05) than normal cell. Treatment with garlic increased fragility in both normal and sickle cell blood however this effect was more pronounced in sickle cell blood. It is recommended that the consumption of garlic in patients with sickle cell anemia should be continuously monitored and carefully regulated.
Allium Sativa (Garlic), Osmotic Fragility, Red Blood Cells, Membrane Stability, Sickle Cell Anemia, % Hemolysis
To cite this article
Salami Hamza Adegoke,
Gadaka Madu Adamu,
John Adeolu Ige,
The Effect of Aqueous Extract of Allium Sativum (Garlic) on Erythrocyte Osmotic Fragility in Normal and Sickle Cell, American Journal of Life Sciences.
Vol. 2, No. 5,
2014, pp. 278-281.
Amagase H., Petsech B.L., Mansuura H. (2001): Intake of garlic and its bioa-ctive components. Journal of Nutrition.131: 9555-9625.
Dubravka S. and Ilona S.V. (2003): An Evolution of the Antioxidant Abilities of Allium Species. Acta Biol Szeged. 47(1-4): 103-106.
Eidi A., Eidi M., ESMALILI E. (2006): Antidiabetic Effect of Garlic (Allium sativum L) in Normal and Streptozotocin Induced Diabetic Rats. Phytomed 13(9-10): 624-629.
Faulknet W.R., King J.W (1970): Manual of Clinical Laboratory Procedures. Clevland OH: Chemical Rubber Company.
Hussein, J.S, Ovaby, F.S and El Shatey N. (2007): Anti Hepatotoxic Effect of Garlic and Onion Oils on Ethanol-Induced Liver Injury in Rats. J of Appl Sci Res. 3(l1): 1527-53.
Jaiswal N. and Rizvi I.S. (2011); Protective Effect of Different Layers of Onion Extracts (Allium Cepa L.) on Markers of Oxidative Stress in Erythrocytes. Cell Memb. Free Rad. Res. 3(2): 171-177
Kato G.I., Gladwin M.T. (2009) Mechanism and Clinical Complications of Hemolysis in Sickle Cell Disease and Thalassemia. In Steinberg M.H., Forget B.G., Higgs D.R., Nagel R.I. editors: Disorders of Hemoglobin Genetic, Pathophysiology and Clinical Management. New York Cambridge University Press.
Nolan. V.G., Wyszynski D.F., Farrer L.A., Steinberg M.H., (2005) Hemolysis-associated Priapism in Sickle Cell Disease. Blood 106(9): 3264-3267.
Rai, D.K., Rai P;K., Rizui S., Watal G., Shama B., (2009): . Carbofuran Induced Toxicity in Rats: Protective Role of Vitamin C. Experimental Toxicology and Pathology. 612531 535.
Rother R.P., Bell., Hillmen P., Gladwin M.T., (2005): The Clinical Sequelae of Intravascular Hemolysis and Extra Cellular plasma Hemoglobin. A novel Mechanism of Human Disease. JAMA J. American Medical Association 295(13): 1653-1662.
Salami H.A., Ekanem A.U. and John A.I., (2012): The Effect of Aqueous Preparation of Allium Cepa (Onion) and Allium Sativa (Garlic) on Erythrocyte Osmotic Fragility in Wistar Rats: In Vivo and In Vitro Studies. Nig. J. Physiol. Sci. 27(1): 29-34.
Silva H.G.D., Junior B.E., Almeida A.E. and Bonini-Domingos R.C. (2013): Oxidative Stress in Sickle Cell Disease: An Overview of Erythrocyte Redox Metabolism and Current Antioxidant Therapeutic Strategies. Free Rad. Biol. Med. 65: 1101- 1109.
Singh V. K., Singh D.K (2008): Pharmacological Effects of Garlic (Allium sativum L.). Annu Rev Biomed Sci; 10:6-26.
Steiner M., and Li W. (2001): Garlic as an Antithrombotic Agent. J. Nutri. 131(35): 980-984.
Takasu J., Uykimpang R., Sunga M. A., Amagase H. and Niihara Y. (2006): Aged Garlic Extract is a Potential Therapy for Sickle Cell Anemia. J. Nutr. 803S-805S.