To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells
American Journal of Life Sciences
Volume 2, Issue 3, June 2014, Pages: 176-181
Received: Apr. 5, 2014; Accepted: Apr. 26, 2014; Published: Jun. 30, 2014
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Karen Poon, Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China
Tse Chiu Chung, Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China
Chang Xu, Program of Food Science and Technology, Division of Science and Technology, BNU-HKBU United International College, 28 Jinfeng Road, Tangjiawan, Zhuhai, Guangdong, China ; Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, P.R. China
Ruihua Wang, Department of Gastroenterology, Shanghai Jiao Tong University affiliated Sixth People’s Hospital South Campus, 6600 Nanfeng Road, Fengxian District, Shanghai, China
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Proton leak has been implicated in various chronic diseases like diabetes and cancer. In this study, current from intact cells, including mice liver cells, pig blood cells and human breast cancer cell MCF-7 were measured by microbial fuel cells (MFC). Positive current change in normal liver cells were induced by either 2,4-dinitrophenol (DNP) or Piceatonnol. The effect of DNP in enhancing the proton conductivity would increase the degree of positive current change, while Piceatonnol in improving the mitochondria membrane potential would support the sustainability of the positive current change with time. Piceatonnol was found to be more effective in inducing positive current change in cancer cells than in liver cells. The higher effectiveness of Piceatonnol to cancer cells would be explained by the high proton leak condition of the cells, and so increased the current production. Little positive current change could be induced in red blood cell by either DNP or Piceatonnol. Results supported the hypothesis of the high mitochondria membrane potential to support the positive current change in cells with time, while the proton conductivity determined the degree of positive current change. The condition of proton leak of cells seemed to be the limiting factor for the positive current change in cells.
Proton Leak, Electron Leak, Microbial Fuel Cells, 2,4-Dinitrophenol, Piceatonnol
To cite this article
Karen Poon, Tse Chiu Chung, Chang Xu, Ruihua Wang, To Investigate the Correlation of Proton Leak and Current Produced from Animal Cells by Microbial Fuel Cells, American Journal of Life Sciences. Vol. 2, No. 3, 2014, pp. 176-181. doi: 10.11648/j.ajls.20140203.17
Hames, D. & Hooper, N., “Electron Transport and Oxidative Phosphorylation” In: Biochemistry. s.l.:Garland Science, pp. 398-412, 2011.
Li, X. , Zhao, J. “An Approach to the Nature of Qi in TCM-Qi and Bioenergy.” In: H. Kuang, ed. Recent Advances in Theories and Practice of Chinese Medicine. S.l: InTech, pp 79-106, 2012.
Byrd-Bredbenner, C., Moe, G., Beshgetoor, D. & Berning , J. eds. “Energy Metabolism” In: Wardlaw's Perspectives in Nutrition. The Eighth Edition ed. s.l.:McGraw-Hill, pp. 281-291, 2009.
Skulachev VP “Uncoupling: new approaches to an old problem of bioenergetics.” Biochim. Biophys. Acta 1363, 100–124, 1998.
Trzcionka M; Withers KW; Klingenspor M; Jastroch M, “The effects of fasting and cold exposure on metabolic rate and mitochondrial proton leak in liver and skeletal muscle ofan amphibian, the cane toad Bufo arinus.” J Exp Biol. 211: pp. 1911-8, 2008.
Brand MD, Affourtit C, Esteves TC, Green K, Lambert AJ, Miwa S, Pakay JL, Parker N “Mitochondrial superoxide production, biological effects, and activation of uncoupling proteins” Free Radic Biol Med 37: 755-767, 2004.
Baffy G “Uncoupling protein-2 and cancer. Mitochondrion” 10: 243–252, 2010.
Ajit S. Divakaruni and Martin D. Brand. “The Regulation and Physiology of Mitochondrial Proton Leak.” Physiology 26: 192–205, 2011.
Liu S; Jiao X; Wang X; Zhang L “Interaction of electron leak and proton leak in respiratory chain of mitochondria--proton leak induced by superoxide from an electron leak pathway of univalent reduction of oxygen.” Sci China C Life Sci. Vol. 39 (2), pp. 168-78, 1996.
Bevilacqua L., Ramsey J.J., Hagopian K., Weindruch R. , Harper M. “Long-term caloric restriction increases UCP3 content but decreases proton leak and reactive oxygen species production in rat skeletal muscle mitochondria” American Journal of Physiology - Endocrinology and Metabolism. 289. E429-E438, 2005.
Derdak Z, Mark NM, Beldi G, Robson SC, Wands JR, Baffy G “The mitochondrial uncoupling protein-2 promotes chemoresistance in cancer cells.” Cancer Res 68: 2813–2819, 2008.
Brand MD “The proton leak across the mitochondrial inner membrane” Biochim Biophys Acta 1018: 128-133, 1990.
Antonenko YN, Khailova LS, Knorre DA, Markova OV, Rokitskaya TI, “Penetrating Cations Enhance Uncoupling Activity of Anionic. Protonophores in Mitochondria.” PLoS ONE 8(4): e61902. 2013 doi:10.1371/journal.pone.0061902.
Balaban RS, Nemoto S, Finkel T “Mitochondria, oxidants, and aging.” Cell 120, 483–495, 2005.
Caldeira da Silva CC, Cerqueira FM, Barbosa LF, Medeiros MH, Kowaltowski AJ “Mild mitochondrial uncoupling in mice affects energy metabolism, redox balance and longevity.” Aging Cell 7: 552–560, 2008.
Turrens JF “Mitochondrial formation of reactive oxygen species.” J. Physiol. 552, 335–344, 2003.
Starkov AA, Fiskum G, Chinopoulos C, Lorenzo BJ, Browne SE, Patel MS, Beal MF “Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species.” J. Neurosci. 24, 7779–7788, 2004.
Tretter L, Adam-Vizi V “Generation of reactive oxygen species in the reaction catalyzed by α-ketoglutarate dehydrogenase.” J. Neurosci. 24, 7771–7778, 2004.
Tahara EB, Barros MH, Oliveira GA, Netto LES, Kowaltowski AJ “Dihydrolipoyl dehydrogenase as a source of reactive oxygen species inhibited by caloric restriction and involved in Saccharomyces cerevisiae aging. “FASEB J. 21, 274–283, 2007.
Baffy G, Z Derdak and SC Robson “Mitochondrial recoupling: a novel therapeutic strategy for cancer?” British Journal of Cancer 105, 469-474, 2011.
Hartwell, J. L. Lloydia 32, 153–205, 1969.
Zheng Jianbiao and Victor D. Ramirez. “Piceatannol, a Stilbene Phytochemical, Inhibits Mitochondrial F0F1-ATPase Activity by Targeting the F1 Complex,” Biochemical and Biophysical Research Communications 261, 499–503, 1999.
Smedsrød B “Protocol for Preparation of Mouse Liver Kupffer Cells and Liver Sinusoidal Endothelial cells “ 2012
Soule, HD; Vazquez J, Long A, Albert S, Brennan M. “A human cell line from a pleural effusion derived from a breast carcinoma.” Journal of the National Cancer Institute 51 (5): 1409–1416, 1973.
Logan B. “Voltage generation”. In Microbial Fuel cell, John Wiley & Son, Inc. New Jersey pp. 29-43, 2008.
Li Z., Zhang X., and Lecheng L.L. “Electricity production during the treatment of real electroplating wastewater containing Cr6+ using microbial fuel cell.” Process Biochem. 43: 1352-1358, 2008.
Song, Z., Wang, D. “Proton Leak and its Role in Basal Metabolism.” Prog. Biochem. Biophys., 28, pp. 474-477, 2001.
Weindruch R and Sohal RS. “Caloric intake and aging.” N Engl J Med 337: 986–994, 1997.
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