Prevalence of ESBL, AmpC and Carbapenemase among Gram Negative Bacilli Isolated from Clinical Specimens
American Journal of Life Sciences
Volume 2, Issue 2, April 2014, Pages: 76-81
Received: Mar. 12, 2014;
Accepted: Apr. 9, 2014;
Published: Apr. 20, 2014
Views 3371 Downloads 272
Vijaya Doddaiah, Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B. G. Nagara, Karnataka, India
Dhanalakshmi Anjaneya, Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B. G. Nagara, Karnataka, India
Background: Antimicrobial resistance is a growing threat worldwide. The predominant mechanisms for resistance to the β-lactam antibiotics in gram negative bacilli is the production of β-lactamases. Aim: To determine the prevalence of ESBL, AmpC and carbapenemase production among GNB isolated from various clinical samples. Materials and Methods: A total of 378 GNB isolated were identified and processed for the detection of ESBL, AmpC and Carbapemase production using various methods. Results: Out 378 GNB 197 (52.12%) showed the presence of one or more β-lactamases and 181 (47.88%) were negative. 33.86%, 14.24% and 18.25% showed the presence of ESBL, AmpC and Carbapenemase among the 378 GNB studied. Conclusion: This study highlights the prevalence of ESBL, AmpC and Carbepenemase producing GNB in a rural tertiary care teaching hospital
Prevalence of ESBL, AmpC and Carbapenemase among Gram Negative Bacilli Isolated from Clinical Specimens, American Journal of Life Sciences.
Vol. 2, No. 2,
2014, pp. 76-81.
Umadevi S, Kanhakumari G, Joseph NM, Kumar S, Eaow JM, Atephen S, Singh UK. Prevalence and antimicrobial susceptibility pattern of ESBL producing gram negative bacilli. J Clin Diag Res 2011; 5(2): 236-239.
Shoorashetty RM, Nagarathnamma T, Prathibha J. Comparison of the boronic acid disk potentiation test and cefepime-clavulanic acid method for the detection of ESBL among AmpC-producing Enterobacteroaceae. Indian J Med Microbiol 2011; 29 (3) :297-30.
Jacoby GA. AmpC β-Lactamases. Clin Microbiol Rev 2009;22:161-82.
Jesudason MV, Kandathil AL, Balaji V.Comparison of two methods to detect carabapenemase and matallo-β-lactamase production in clinical isolates. Indian J Med Res 2005;121:780-3
Galani I, Rekatsina PD, Hatzaki D, Plachouras D, Souli M, Giamarellou H. Evaluation of different laboratory tests for the detection of metallo β-lactamase production in Enterobacteriaceae. J Antimicrob Chemother2008;61:548-53.
Valsan C, Chinnan JP, Sathiavathy KA. Phenotypic detection of ?-lactamases in enterobacreriaceae using a 12-disk procedure. J Acad Clin Microbiol 2013;15(1):7-10.
Collee JG, Fraser AG, Marmion BP, Simmons A. Mackie & McCartney Practical Medical Microbiology. 14th ed. Edinburgh (UK): Churchill Livingston;1999
Clinical and Laboratory Standards. Performance Standards for Antimicrobial susceptibility Testing; Twentieth Informational Supplement. CLSI Document M 100-S20, Wayne, PA: CLSI,Wayne,PA,USA,2010.
Singhal S, Mathur T, Khan S, Upadhyay DJ, Chugh S, Gaind, Rattan A. Evaluation of Methods for AmpC Beta-Lactamase in gram negative clinical isolates from tertiary care hospitals. Indian J Med Microbiol. 2005;23(2): 120-124.
Nirav P, Pandya, Sweta B, Prajapati, Sanjay J, Mehta et al. Evalution of various methods for detection of metallo-? -lactamase (MBL) production in Gram negative bacilli.Int J Biol Med Res 2011;2(3):775-777 .
Rai S, Manchanda V, Singh NP, Kaur IR. Zine-dependent carbepenemases in clinical isolates of family Enerobacteriaceae. Indian J Med Microbiol 2011; 29(3):275-9.
Datta P, Gupta V, Garg S, Chander J. Phenotipic method for differentiation of carbapenemases in Enterobacteriaceae; study from North India. Indian J Pathol Microbiol 2012;55:157-60
Shanthi M, Sekar U, Arunagiri K, Sekar B. Detection of AmpC genes encoding for beta-lactamases in Escherchia coli and Klebsiella pneumonia. Indian J Med Microbiol 2012;30:290-5.
Babypadmini S, Appalaraju B. Extended-spectrum beta-lactamases in urinary isolates of coli and Klebsiella pneumonia- Prevalence and susceptibility in a tertiary care hospital. Indian J Med Microbiol 2004;22:172-4.
Paul Schrekenberger Escherichia. Phenotypic Detection of β-Lactamase resistance in gram negative bacilli. Testing and Interpretation Guide. Available from: http://www.scacm.org/phenotypic Detection Antibiotic resistance _rev5.pdf (Last reviewed on 2012 Fseb 21)
Behera B,Mathur P,Das A,Kapil A,Sharma V. An evaluation of four phenotypic techniques for detection of mettalo bête lactamase producing Pseudomonas aeruginosa. Indian J Med Microbiol 2008;26(3):233-37.