Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items
American Journal of Life Sciences
Volume 1, Issue 6, December 2013, Pages: 267-272
Received: Nov. 18, 2013; Published: Dec. 20, 2013
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Authors
Nishat Sarker, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Sharmin Islam, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Mehedi Hasan, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Farzana Kabir, Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b), Dhaka, Bangladesh
Md. Aftab Uddin, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
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Abstract
Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts. Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with an array of bacteria ranging between 3.24×102 -6.5×109 cfu/g. Results of multiplex polymerase chain reaction (PCR) assay revealed that out of 130 isolates of E. coli screened from all categories of food items, 52 (13%) were found to be enterotoxigenic E. coli, 32 (8%) were the Shiga toxin producing E. coli isolates, 14 (3.5%) were enteropathogenic and 6 (1.5%) were found to be enteroinvasive isolates. Results of plasmid profiling of the isolates was further in agreement to the presence of different pathogenic E. coli strains.
Keywords
Street Vended Foods, Microbiological Spoilage, Escherichia coli, Antibiotic Resistance, Multiplex Polymerase Chain Reaction (PCR)
To cite this article
Nishat Sarker, Sharmin Islam, Mehedi Hasan, Farzana Kabir, Md. Aftab Uddin, Rashed Noor, Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items, American Journal of Life Sciences. Vol. 1, No. 6, 2013, pp. 267-272. doi: 10.11648/j.ajls.20130106.15
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