Alleviation of Salinity Stress in White Corn (Zea mays L.) Plant by Exogenous Application of Salicylic Acid
American Journal of Life Sciences
Volume 1, Issue 6, December 2013, Pages: 248-255
Received: Nov. 13, 2013; Published: Nov. 30, 2013
Views 3996      Downloads 222
Mona A. Ismail, Biotechnology Department, Faculty of Science, Taif University, Saudi Arabia; Botany Department, Faculty of Science, Suez Canal University, Egypt
Article Tools
Follow on us
This experiment was conducted to study the effect of exogenous application of Salicylic Acid ( 200 ppm) to alleviate the damage in Zea mays L. plants under different NaCl doses (20, 40, 60 and 100 mMol). Shoot and root lengths, fresh and dry weights, leaf area, chlorophyll a, chlorophyll b, total chlorophyll, chlorophyll stability index were measured. The antioxidant enzymes (lipid peroxidase and glutathione) activities were estimated. NaCl significantly (P > 0.05) reduced all measured growth parameters, photosynthetic efficiency and antioxidant oxidative enzyme contents. Exogenous application of SA alleviated the inhibitory effects of NaCl on Zea Mays plants. SA enhanced plant salt tolerance in terms of improving the measured plant growth criteria. Moreover, the antioxidant enzyme contents were enhanced in response to NaCl and/or SA treatment providing s synergistic interaction. The toxic effects generated by the lower concentration of NaCl (20mM) were completely overcome by the application of SA .SA ameliorated the stress generated by NaCl through the antioxidant system and the stability of the photosynthetic process.
Zea Mays, Salinity, Salicylic Acid, Photosynthesis, Growth, Glutathione, Lipid Peroxidase
To cite this article
Mona A. Ismail, Alleviation of Salinity Stress in White Corn (Zea mays L.) Plant by Exogenous Application of Salicylic Acid, American Journal of Life Sciences. Vol. 1, No. 6, 2013, pp. 248-255. doi: 10.11648/j.ajls.20130106.12
I. Afzal, S.M.A. Basra, M. Farooq and A. Nawaz (2006). Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Int. J. Agri. Biol., 8: 23-28.
M. Arfan, (2009). Exogenous application of salicylic acid through rooting medium modulates ion accumulation and antioxidant activity in spring wheat under salt stress. Int. J. Agri. Biol., 11: 437-442.
M. Arfan, A. Habib and M. Ashraf.( 2007). Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress J. Plant Physiol., 164(6): 685-694.
D. T. Arnon, (1994). Copper enzyme in isolated chloroplasts, polyphenaloxidase in Beta vulgaris. Plant Physiol., 24: 1-15.
M. Ashrafuzzaman, M.A.H. Khan and S.M. Shahidullah, (2002). Vegetative growth of maize (Zea mays) as affected by a range of salinity. Crop Res. Hisar, 24: 286–91
Carleton and Foote.( 1965). A comparison of methods for estimating total leaf area of barley plants. Crop Sci., 5(6): 602-603
N. Chakrabarti , S. Mukher Jee (2003) . Effect of phytohormones pre –treatment on nitrogen metabolism in vigno radiate under salt stress.Biol.plant. 36, 63-66.
M. Chance, and A. C. Maehly. (1955). Assay of catalases and peroxidases. Methods Enzymol., 2:764-817.
Z. Chen, S. Iyer, A. Caplan, D.F. Klessig and B. Fan. (1997). Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues. Plant Physiol., 114: 193-201.
S. Choudhury, & S. K. Panda( 2004). Role of salicylic acid in regulating cadmium induced oxidative stress in Oryza sativa L. roots. Bulg. J. Plant Physiol., 30 (3-4), 95-110.
H. E. Deef, (2007). Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Advances in Biological Research, 1 (1-2): 40-48.
TJ. Flowers, TD. Colmer( 2008). Salinity tolerance in halophytes. New Phytol 179:945–963
CH . Foyer, B . Halliwell (1976). The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbate acid metabolism. Planta 133: 21-25
B .Guan, Hu Y. Zeng, Y. Wang, F. Zhang (2011). Molecular characterization and functional analysis of a vacuolar Na+/H+ antiporter gene (HcNHX1) from Halostachys caspica. Mol Biol Rep 38:1889–1899
A. Gunes., A. Inal , M. Alpaslan, , N. Cicek, E. Guneri , F..Eraslan, T. Guzelordu ( 2005). Effects of exogenously applied salicylic acid on the induction of multiple stress tolerance and mineral nutrition in maize (Zea mays L.). Arch. Agron. Soil Sci. 51, 687-695.
Y. Gunes , A. Inal, M. Alpaslan, F. Eraslan, E.G. Bagci and G.N. Cicek (2007). Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. J. of Plant Physiol.164:728-736
AM . Hamada (1996). Effect of NaCl, water stress or both on gas exchange and growth of wheat. Biol. Plant. 38: 405-412.
A.A. Hassanein ( 2000). Physiological responses induced by shock and gradual salinization in rice (Oryza sativa L.) seedlings and the possible roles played by glutathione treatment. Acta Botanica Hungarica, 42(1-4): 139-159.
S. Hayat, H. Ahmad (2007). Salicylic acid: A Plant Hormone. Springer, Dordrecht, The Netherlands
RL. Heath, L. Packer (1968). Photoperoxidation in isolated chloroplasts: I. kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
D.R.. Hoagland, and D.I Arnon (1950). The water culture method for growing plants without soil. California Agri. Exp. Stat. Circ., 347: 32
T. Janda, E. Horvath, G. Szalai, E. .Paldi (2007). Role of salicylic acid in the induction of abiotic stress tolerance. In S. Hayat, A. Ahmad (eds.), hormone, Salicylic acid: A plant (91 – 150). Springer, Dordrecht, The Netherlands.
T. Janda, E. Horvath, G. Szalai, E. Paldi (1999). Hydroponic treatment with salicylic acid decrease the effects of chilling injury in maiz ( zea mays L. ) plants .planta, 208:175-180
MN . Jithesh, SR. Prashanth, KR. Sivaprakash, AK. Parida (2006) Antioxidative response mechanisms in halophytes: their role in stress defence. J Genet 85:237–254
S.U. Khan, A. Bano, J. Din and A.R. Gurmani. 2012. Abscisic acid and salicylic acid seed treatment as potent inducer of drought tolerance in wheat (Triticum aestivum L.). Pak. J. Bot., 44(1): 43 49.
M Kumari, MD sam, Y virnala, A. Pawan (2004) . physiological parameters governing drought in maize . Indian J plant physiol.:203-207.
J. D Maguire (1962) "Speed of Germination-Aid in Selection and Evaluation for Seedling Emergence and Vigor," Crop Science, Vol. 2, No. 2, , pp. 176-177.
E.V. Mass, and G.J. Hoffman. (1977). Crop salt tolerance current assessment. J. Irrig. and Drain Div. ASCE 103: 115-134
G. Merkouropolous, D. C. Barnett, A. H. Shirasat (1999). The Arabidopsis expensin gene is developmentally regulated, is induced by wounding, methyl jasmonate, abscicissic acid, salicylic acid and codes for protein with unusual motifs. planta, 208,212-219
G. Miller, N. Suzuki, S. Ciftci-Yilmaz, R. Mittler ( 2010). Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467
C. Miyake, K. Asada (1992). Thylakoid bound ascorbate peroxidase in Monodehydroascorbate radicals in the thylakoids. Plant Cell Physiol. 33: 541-553.
R .Munns, M .Tester (2008). Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
G. Nector, CH. Foyer (1998). Ascorbate and glutathione,keeping active oxygen under .Annu Rev. Plant Physiol,Mol.Biol.4249-279
S. Noreen, M. Ashraf, M. Hussain and A. Jamil.( 2009). Exogenous application of Salicylic acid enhances antioxidative capacity in salt stressed sunflower (Helianthus annus L.) plants. Pak. J. Bot., 41(1): 473-479.
E. Paterniani, (1990). Maize breeding in tropics. Cri. Rev. Plant Sci., 9: 125-154.
F. Pérez-Alfocea, MT . Estan, M. Caro, and G. Guerrier (1993). Osmotic adjustment in Lycopercon esculentum and L. pennelli under NaCl and polyethylene glycol 6000 iso-osmotic stress stresses. Physiol. Plant.87:493-498
MG . Pitman, A . Läuchli (2002). Global impact of salinity and agricultural ecosystems. In: Läuchli A, Lüttge U (eds) Salinity: environment – plants – molecules. Kluwer, Dordrecht, pp 3–20 Plant. 87: 493-498
G.G. Rao, and G.R. Rao,( 1981). Pigment composition and chlorophylase activity in pigeon pea (Cajanus indicus Sperng) and gingelley (Sesamum indicum L) under NaCl salinity, Indian , J. Exp. Biol., 19: 768-770.
G. Rao, P. Paliyath, D.P. Ormrod, C.B. Murr and Watkins. (1997). Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes. Plant Physiol., 115:137-149.
M. Schwarz (1985). Antioxidan systems and plant responses to the environment . In: Simiroff N ed. Enviroment and plant metabolism.
T. Senaratna, D. Touchell, E. Bunn, K. Dixon ( 2000). Acetyl salicylic acid ( Asperrin ) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul.30,157-161.
S. Shabala, A. Mackay (2011). Ion transport in halophytes. In: Kader JC, Delseny M (eds) Advances in botanical resea rch, Elsevier, Amsterdam, 57:151–199
J. Song, X. Ding, G. Feng, F. Zhang (2006) Nutritional and osmotic roles of nitrate in a euhalophyte and a xerophyte in saline conditions. New Phytol 171:357–366
R. G. D. Steel, and J. H. Torrie.(1983). Principles and Procedures of Statistics. A Biometrical Approach. Mc Graw Hill, Book Inc., New York, USA. Pp 633.
FH. Witham, BF. Blaydes, RM. Devlin (1971). Experiments in plant physiology, Van Nostrand Reinhold, New York, USA, 1971, pp 167-200
N. Yalpani, AJ . Enyedi, J. Leon, I. Raskin (1994) .Ultraviolet light and ozone stimulate accumulation of salicylic acid, pathogenesis-related proteins and virus resistance in tobacco. Planta 193: 372-376.
JK. Zhu. (2001). Plant salt tolerance. Trends Plant Sci 2001(6):66–71
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186