Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB)
American Journal of Life Sciences
Volume 1, Issue 6, December 2013, Pages: 243-247
Received: Aug. 30, 2013;
Published: Nov. 30, 2013
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Sanjay R. Biradar, Tissue Culture Laboratory, Department of Botany, Shri Chhatrapati Shivaji College Omerga – 413606, Dist. Osmanabad, (MS).INDIA
Bhagyashri D. Rachetti, Tissue Culture Laboratory, Department of Botany, Shri Chhatrapati Shivaji College Omerga – 413606, Dist. Osmanabad, (MS).INDIA
Centella asiatica (L.) Urb. known as Brahmi, Indian Pennywort and Mandookaparni is a small herbaceous annual plant of the family Apiaceae, It is used in traditional medicine for the treatment of various ailments & ayurvedic treatment for many diseases. Present study deals with the qualitative and quantitative analysis of ethanolic extract of root, stem & leaf of Centella asiatica (L.) Urb. A qualitative analysis by thin layer chromatography & a quantitative analysis by standard chemical protocol of secondary metabolites in the root, stem and leaf of the Centella asiatica L. (URB) have been studied. Using thin layer chromatography (TLC) different components like Alkaloids, Saponin, Flavonoids, Terpenoides, Phenol & Tannin are isolated & identified. The Rf values of the developed spots in the different solvent systems are noted. In the quantitative analysis, alkaloids, saponins, terpenoids & flavonoids are extracted by using the standard chemical protocol. These results may be helpful for rationale use of this plant in the modern system of health care.
Sanjay R. Biradar,
Bhagyashri D. Rachetti,
Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB), American Journal of Life Sciences.
Vol. 1, No. 6,
2013, pp. 243-247.
Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001; 49(11): 5165-5170.
Cai YZ, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem 2003; 51(8): 2288-2294.
Sala A, Recio MD, Giner RM, Manez S, Tournier H, Schinella G, Rios JL. Antiinflammatory and antioxidant properties of Helichrysum italicum. J Pharm Pharmacol 2002; 54(3): 365-371.
Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative activities of Plant-derived polyphenolic flavonoid. Free radical Res 1995; 22: 375-383.
Ashokkumar D, Mazumder UK, Gupta M, Senthilkumar GP, Selvan VT. Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in various in vitro Models. J Comp Integ Med 2008; 5(1): 1-6.
Veerapur VP, Prabhakar KR, Parihar VP, Kandadi MR, Ramakrishana S et al. Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector. Evid Based Complement Alternat Med 2009; 6(3): 317-324.
Kitts DD, Yuan YV, Wijewickreme AN, Hu C. Antioxidant properties of a North American gingseng extract. Mol Cell Biochem 2000; 20(3):1-10.
Muselík J, García-Alonso M, Martín-López MP, Želmièka M, Rivas-Gonzalo JC. Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Antocyanins and Piranoantocyanins. Int J Mol Sci 2007; 8: 797-809.
Wang SY, Jiao H. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. J Agric Food Chem 2000; 48: 5672-5676.
Singh.S., Gautam.A, Sharma.A and Batra.A Centella asiatica (L.): A plant with immense medicinal potential but threatened, International journal of pharmaceutical sciences review and research 2010; 4(2): 9-17.
Loiseau, A. and Mercier, M. Centella asiatica and skin care. Cosmetics and Toiletries Magazine 2000; 115: 63- 67.
Brinkhaus B., Lindner M., Schuppan D. and Hahn E.G., Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica, Phytomedicine, 7(5), 427-48 (2000)
Zheng, CJ. and Qin, LP. Chemical components of Centella asiatica and their bioactives. Journal of Chinese Integrative Medicine 2007; 5: 348-351.
Abdulrahman F, Inyang SI, Abbah J, Binda L, Amos S, Gamaniel K (2004). Effect of aqueous leaf extracts of Irvingia gabonesis on gastrointestinal tracts of rodents. India J. Exp. Biol. 42:787-791.
Harborne JB, Phytochemical methods: A Guide to Modern techniques of plants Analysis. Chapman and Hall London, UK. 1998.
Harborne JB. Phytochemical methods, London. Chapman and Hall, Ltd. 1973; 49-188.
Boham BA and Kocipai-Abyazan R. "Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium vaticulatum and V. calycinium" Pacific Science (1974) 48: 458-463.
Obdoni BO and Ochuko PO. "Phytochemical studies and comparative efficacy of the crude extracts of some Homostatic plants in Edo and Delta States of Nigeria" Global J. Pure Appl. Sci. (2001) 8: 203-208.
Ferguson NM. A Text book of Pharmacognosy. Mac Milan Company, New Delhi, 1956, 191.