Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam
American Journal of Life Sciences
Volume 1, Issue 1, February 2013, Pages: 14-21
Received: Feb. 7, 2013;
Published: Feb. 20, 2013
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Cao Ngoc Diep, Dept. Microbiology Biotechnology, Can Tho City, Vietnam; Institute of Marine Biochemistry, Ha Noi city, Vietnam; Biotechnology R&D Intitute, Can Tho University, Can Tho City, Vietnam; Vietnam Academy Institute of Science and Technoloy, Ha Noi city, Vietnam
Pham Viet Cuong, Dept. Microbiology Biotechnology, Can Tho City, Vietnam; Institute of Marine Biochemistry, Ha Noi city, Vietnam; Biotechnology R&D Intitute, Can Tho University, Can Tho City, Vietnam; Vietnam Academy Institute of Science and Technoloy, Ha Noi city, Vietnam
A total of 2318 heterotrophic nitrogen removal (HNR) bacteria isolated from piggery wastes (after biogas container) were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates) and heterotrophic nitrifying and denitrifying bacteria (569 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria) and Klebsiella (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bac-teria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from piggery wastes, showed a very diverse community of HNR bacteria with a relatively high number of species involved in solid-wastewater samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.
Cao Ngoc Diep,
Pham Viet Cuong,
Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam, American Journal of Life Sciences.
Vol. 1, No. 1,
2013, pp. 14-21.
D. Obaja, S. Mace, J. Costa, J. Sans, and J. Mata-Alverez, "Nitrification, denitrification and biological phosphorus re-moval in piggery wastewater using a sequencing batch reactor" Biores. Technology, vol 87, pp.103-111, 2003.
H.S. Joo, M. Hirai, and M. Sinoda, "Piggery wastewater treatment using Alcaligenes faecalis strain No. 4 with hete-rotrophic nitrification and aerobic denitrification" Water Res., vol 40, pp. 3029-3036, 2006.
J.J. Su, R.S. Yeh, and P.W. Tseng, "A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan", Curr. Microb. vol. 53, pp. 77-81, 2006.
L.A. Robertson, E.W.J. Van Neil, R.A.M. Torremans, and J.G. Kuenen, "Simultaneous nitrification and denitrification in aerobic chemost at cultures of Thiosphaera pantotropha" Appl. Environ. Microb. vol. 54, pp. 2812-2818, 1988.
H.S. Joo, M. Hiral, and M. Sinoda, "Characteristics of am-monium removal by heterotrophic nitrofication-aerobic de-nitrification by Alcaligenes faecalis No 4", J. Biosci. Bioeng. vol. 100, pp. 184-191, 2005.
J.K. Kim, K.L. Park, R.S. Cho, S.W. Nam, T.J. Park, and R. Bajpal, "Aerobic nitrification-denitrification by heterotrophic Bacillus strains. Biores. Technol.", vol. 96, pp. 1897-1906, 2005.
B. Zhao, Y.L. He, J. Hughes, and X.F. Zhang, "Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoa-ceticus HNR", Biores. Technol., vol.101, pp. 5194-5200, 2010.
H.J. Hoben, and P. Somasegaran, "Comparison of Pour, Spread and Drop Plate Methods for Enumeration of Rhizobium spp. in Inoculants made from Presterilized peat".,. Appl. Environ. Microbiol. vol. 44, pp. 1246-1247, 1982.
B. Neumann, A. Pospiech, and H.U. Schairrer, "Rapid isola-tion of genomic DNA from Gram-negative", Trends Gent. vol. 8, pp. 332-333, 1982.
S. Turner, K.M. Pryer, V.P.M. Miao, J.D. Palmer, "Investi-gating deep phylogenetic relationships among cyanobacteria and plastids by small subnit rRNA sequence analysis", J. Eukaryotic Microbiol., vol. 46, pp. 327-338, 1999.
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, "MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likehood, Evolutionary Distance and Maximum Parsimony Methods", Mol. Biol. Evol., vol. 28, pp. 2731-2739, 2011.
M.K. Halushka, J.B. Fan, K. Bentley, L. Hsie, N. Shen, A. Weder, R. Cooper, R. Lipshutz, and A. Charavarti, "Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homestasis", Nat. Genet., vol. 22(3), pp. 239-247, 1999.
N. Bernet, N. Delgenes, and R. Moletta,"Denetrification by anaerobic sludge in piggery wastewater", Environmental Technology vol 17, pp. 293-300, 1996.
H. Bothe, G. Jost, M. Schloter, B.B. Ward, and K.P. Witzel, "Molecular analysis of ammonia oxidation and denitrification in natural environments", FEMS Microbiol. Rev. vol. 24, pp. 673-690, 2000.
G.A. Watterson, "On the number of segregation sites in general models without recombination", Theor. Pop. Biol., vol. 7, pp. 256-276, 1975
Rozas, and R. Rozas, "DnaSP version 4.1: an integrated program for molecular population genetics and molecular evolution analysis" Bioinformatics, vol. 15, pp.174-175, 2005.