Heterotrophic Nitrogen Removal Bacteria in Sedimentary and Water of Striped Catfish Ponds In The Mekong Delta, Vietnam
American Journal of Life Sciences
Volume 1, Issue 1, February 2013, Pages: 6-13
Received: Feb. 12, 2013;
Published: Feb. 20, 2013
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Cao Ngoc Diep, Dept. Microbiology Biotechnology, Can Tho City, Vietnam; Institute of Marine Biochemistry, Ha Noi city, Vietnam; Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam; Vietnam Academy Institute of Science and Technology, Ha Noi City, Vietnam
Nguyen Thi Kim Cuc, Dept. Microbiology Biotechnology, Can Tho City, Vietnam; Institute of Marine Biochemistry, Ha Noi city, Vietnam; Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam; Vietnam Academy Institute of Science and Technology, Ha Noi City, Vietnam
A total of 1682 heterotrophic nitrogen removal (HNR) bacteria isolated from sedimentary and water of striped catfish ponds were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (402 isolates), nitrite-oxidizing bacteria (438 isolates), nitrate-oxidizing bacteria (444 isolates) and heterotrophic nitrifying and denitrifying bacteria (398 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Bacillus (low G+C content gram-positive bacteria) and Pseudomonas (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bacteria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from sedimentary and water of striped catfish ponds, showed a very diverse community of HNR bacteria with a relatively high number of species involved in sedimentary and water samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.
Cao Ngoc Diep,
Nguyen Thi Kim Cuc,
Heterotrophic Nitrogen Removal Bacteria in Sedimentary and Water of Striped Catfish Ponds In The Mekong Delta, Vietnam, American Journal of Life Sciences.
Vol. 1, No. 1,
2013, pp. 6-13.
N.T. Phuong, D.T.H. Oanh, and N.A. Tuan, "Striped catfish (Pangasianodon hypophthalmus) Aquacultre in Viet nam: An Unprecedented development within a Decade," Aquaculture in Vietnam: Present Status and Challenges for Sustainable Development. Handbook and Abstracts from Conference at Can Tho University, Can Tho city, Vietnam, p.25, 2008.
G. Van der Vorst, L.M. Triet, P. Sorgeloos, and J. Dewult, "Using life cycle assessment methods and thermodynamics for the evaluation of susyainable processing of Pangasius waste," Aquaculture in Vietnam: Present Status and Challenges for Sustainable Development. Handbook and Abstracts from Conference at Can Tho University, Can Tho city, Vietnam, p.89, 2008.
T.Q. Phu, and Y. Yi, "Anh huong cua viec nuoi ca da tron trong be den chat luong moi truong nuoc o huyen Hong Ngu, tinh Dong Thap," Scientific Journal of Can Tho University, vol. 199, pp. 8-17, 2003 (Vietnamese).
H.D. Park, and D.R. Noguera, "Characteristization of two ammonia-oxidizing bacteria isolated from reactors operated with low dissolved oxygen concentrations," J. Appl. Micro-biol., vol. 102, pp. 1401-1417, 2006.
H.S. Joo, M. Hirai, and M. Sinoda, "Piggery wastewater treatment using Alcaligenes faecalis strain No. 4 with hete-rotrophic nitrification and aerobic denitrification," Water Res., vol. 40, pp. 3029-3036, 2006.
J.J. Su, R.S. Yeh, and P.W. Tseng, "A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan," Curr. Microb., vol. 53, pp. 77-81, 2006.
L.A. Robertson, E.W.J. Van Neil, R.A.M. Torremans, and J.G. Kuenen, "Simultaneous nitrification and denitrification in aerobic chemost at cultures of Thiosphaera pantotropha," Appl. Environ. Microb., vol. 54, pp. 2812-2818, 1988.
H.S. Joo, M. Hiral, and M. Sinoda, "Characteristics of am-monium removal by heterotrophic nitrofication-aerobic de-nitrification by Alcaligenes faecalis No 4," J. Biosci. Bioeng., vol. 100, pp. 184-191, 2005.
J.K. Kim, K.L. Park, R.S. Cho, S.W. Nam, T.J. Park, and R. Bajpal, "Aerobic nitrification-denitrification by heterotrophic Bacillus strains," Biores. Technol., vol. 96, pp. 1897-1906, 2005.
B. Zhao, Y.L. He, J. Hughes, and X.F. Zhang, "Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoa-ceticus HNR," Biores. Technol., vol. 101, pp. 5194-5200, 2010.
H.J. Hoben, and P. Somasegaran, "Comparison of Pour, Spread and Drop Plate Methods for Enumeration of Rhizobium spp. in Inoculants made from Presterilized peat," Appl. Environ. Microbiol., vol. 44, pp. 1246-1247, 1982.
B. Neumann, A. Pospiech, and H.U. Schairrer, "Rapid isola-tion of genomic DNA from Gram-negative," Trends Gent., vol. 8,pp. 332-333, 1992.
S. Turner, K.M. Pryer, V.P.M. Miao, and J.D. Palmer, "In-vestigating deep phylogenetic relationships among cyano-bacteria and plastids by small subnit rRNA sequence analysis," J. Eukaryotic Microbiol., vol. 46, pp. 327-338, 1999.
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, "MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likehood, Evolutionary Distance and Maximum Parsimony Methods," Mol. Biol. Evol., vol. 28, pp. 2731-2739, 2011.
M.K. Halushka, J.B. Fan, K. Bentley, L. Hsie, N. Shen, A. Weder, R. Cooper, R. Lipshutz, and A. Charavarti, "Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homestasis," Nat. Genet., vol. 22(3), pp. 239-247, 1999.
G.A. Watterson, "On the number of segregation sites in general models without recombination," Theor. Pop. Biol., vol. 7, pp. 256-276, 1975.
J. Rozas, and R. Rozas, "DnaSP version 4.1: an integrated program for molecular population genetics and molecular evolution analysis," Bioinformatics, vol. 15, pp. 174-175, 2005.
T.G. Giang, V.N. Ut, and N.T. Phuong, "Bien dong cac yeu to moi truong trong ao nuoi ca tra (Pangasiadon hypophthalmus) tham canh o An Giang," Scientific journal of Can Tho University vol.1, pp. 1-9, 2008 (Vietnamese).
C.N. Diep, P.M. Cam, N.H. Vung, T.T. Lai, and N.T.X. My, "Isolation of Pseudomonas stutzeri in wastewater of catfish fish-ponds in the Mekong Delta and its application for wastewater treatment," Bioresoure Tech., vol. 100, pp. 3787-3791, 2009.
J.J. Su, Y.L. Liu, F.J. Shu, and J.F. Wu, "Treatment of piggery wastewater by contact aeration treatment in coordination of three-step piggery wastewater treatment (TPWT) process in Taiwan," J. Environ. Sci. Health, vol. 32A, pp. 55-73, 1997.
N.N. Nguyen, G.V. Thang, and P.V. Hai, "Survey and research for producing bacterial tablet to treat striped catfish pond environment at intensive scale," Aquaculture in Vietnam: Present Status and Challenges for Sustainable Development. Handbook and Abstracts from Conference at Can Tho University, Can Tho city, Vietnam, p.84, 2008.