Heterotrophic Nitrogen Removal Bacteria in Sedimentary and Water of Striped Catfish Ponds In The Mekong Delta, Vietnam
American Journal of Life Sciences
Volume 1, Issue 1, February 2013, Pages: 6-13
Received: Feb. 12, 2013; Published: Feb. 20, 2013
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Authors
Cao Ngoc Diep, Dept. Microbiology Biotechnology, Can Tho City, Vietnam; Institute of Marine Biochemistry, Ha Noi city, Vietnam; Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam; Vietnam Academy Institute of Science and Technology, Ha Noi City, Vietnam
Nguyen Thi Kim Cuc, Dept. Microbiology Biotechnology, Can Tho City, Vietnam; Institute of Marine Biochemistry, Ha Noi city, Vietnam; Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam; Vietnam Academy Institute of Science and Technology, Ha Noi City, Vietnam
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Abstract
A total of 1682 heterotrophic nitrogen removal (HNR) bacteria isolated from sedimentary and water of striped catfish ponds were classified in four kinds of heterotrophic ammonia-oxidizing bacteria (402 isolates), nitrite-oxidizing bacteria (438 isolates), nitrate-oxidizing bacteria (444 isolates) and heterotrophic nitrifying and denitrifying bacteria (398 isolates). The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the selected HNR bacteria showed high degrees of similarity to those of the GenBank references strains (between 97% and 99.8%). Phylogenetic trees based on the 16S rDNA sequences displayed high consistency, with nodes supported by high bootstrap (500) values. These presumptive HNR isolates were divided four groups that included members of genera Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Bacillus (low G+C content gram-positive bacteria) and Pseudomonas (gram-negative bacteria). Based on Pi value (nucleotide diversity), heterotrophic ammonium-oxidizing bacteria group had highest values and heterotrophic nitrifying-denitrifying bacteria group had the lowest values and Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria group had the highest theta values in comparison of three groups. The present study, the HNR bacteria from sedimentary and water of striped catfish ponds, showed a very diverse community of HNR bacteria with a relatively high number of species involved in sedimentary and water samples and many isolates have nitrogen utilization ability at high concentration (800 – 1200 mM) and high G+C gram-positive bacteria strain occupied higher than low G+C gram-positive bacteria strain.
Keywords
Heterotrophic Nitrogen Removal, 16S Rrna Gene Sequence, Biologic Nitrogen Removal, Sedimentary And Water Of Striped Catfish Ponds, Gram-Positive Bacteria
To cite this article
Cao Ngoc Diep, Nguyen Thi Kim Cuc, Heterotrophic Nitrogen Removal Bacteria in Sedimentary and Water of Striped Catfish Ponds In The Mekong Delta, Vietnam, American Journal of Life Sciences. Vol. 1, No. 1, 2013, pp. 6-13. doi: 10.11648/j.ajls.20130101.12
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