The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise
International Journal of Immunology
Volume 5, Issue 4, August 2017, Pages: 74-79
Received: Jul. 3, 2017;
Accepted: Jul. 18, 2017;
Published: Aug. 11, 2017
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Ehiaghe Friday Alfred, Department of Hematology, College of Health Sciences, Igbinedion University, Okada, Nigeria; Lahor Research and Medical Centre, Benin City, Nigeria; Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Onyenekwe Charles Chinedum, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Ifeanyichukwu Martins, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Akosile Christopher Olusanjo, Department of Medical Rehabilitation, Nnamdi Azikiwe University, Awka, Nigeria
Ehiaghe Imuetiyan Joy, Lahor Research and Medical Centre, Benin City, Nigeria
Nancy Ibeh Chitogu, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Ukibe Nkiruka Rose, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Osakue Nosa, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Ekpe Elijah Chukwuemeka, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
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This study was designed to determine the expression patterns of some pro-inflammatory and anti-inflammatory cytokine genes in some young male undergraduates that passed through exhaustive exercise using the Bruce protocol for sub-maximal acute exercise as studies have shown that exhaustive exercise activates the release of multiple cytokine genes which modulate the immune responses of stressed individuals. Twenty five (25) healthy young male undergraduates with an average age of 24.3±3 years and body mass index of 22.7±1.8 (Kg/m2) participated fully in the study. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Tumor necrosis factor α, interferon gamma and interleukin-10 genes expression patterns were detected using reverse transcriptase polymerase chain reaction methods. Serum cortisol (nmol/L) using enzyme-linked immunosorbent assay method. The study revealed that eleven (11) out of the twenty-five (25) participants had their tumor necrosis factor α genes up-regulated at 1 hour post exercise and sustained till 24 hours post exercise. Moreover, interferon gamma and interleukin-10 genes were up-regulated at 4 hours post exercise and sustained till 24 hours post exercise in most of the subjects. This study also observed that the cortisol level of the exercised subjects was significantly higher at 1 hour post acute exercise when compared with pre-exercise stage. Acute exercise was associated with an enhanced lymphocytic cell functions which are evidence in the up-regulation patterns of both lymphocytic pro-inflammatory cytokine and anti-inflammatory cytokine genes post exercise.
Tumor Necrosis Factor Alpha Genes, Exercise, Lymphocytic Cell Functions, Cytokines, Cortisol
To cite this article
Ehiaghe Friday Alfred,
Onyenekwe Charles Chinedum,
Akosile Christopher Olusanjo,
Ehiaghe Imuetiyan Joy,
Nancy Ibeh Chitogu,
Ukibe Nkiruka Rose,
Ekpe Elijah Chukwuemeka,
The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise, International Journal of Immunology.
Vol. 5, No. 4,
2017, pp. 74-79.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abdalla AO, Kiaii S, Hansson L, Rossmann ED, Jeddi-Tehrani M, Shokri F, Osterborg A, Mellstedt H, Rabbani H. Kinetics of cytokine gene expression in human CD 4+ and CD 8+ lymphocyte subsets using quantitative real-time PCR. Scandinavian Journal of Immunology. 2003; 58: 601- 606.
Katia C, Michela F, Stefano C, Giacomo S, Maurizio SAV. Exercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization. BMC Molecular Biology. 2008; 9: 49-51.
Bermudez DM, Canning DA, Liechty KN. Age and pro-inflammatory cytokine production: Wound-healing implications for scar-formation and the timing of genital surgery in boys. Journal of Pediatric Urology. 2011; 7: 324- 331.
Josef F. Biomarkers of peripheral muscle fatigue during exercise. Musculoskeletal Disorders. 2012; 13: 213- 218.
Elenkor IJ and Chrousos E. Stress hormones, proinflammatory and anti-inflammatory Cytokines and autoimmunity. Annals of Academic Science. 2002; 966:290-303.
Ehiaghe FA, Agbonlahor DE, Ositadima MI, Ehiaghe IJ, Osadolor HB. Effect of academic stress on serum cortisol and CD 4 cell count in young male post graduate students in Okada, Nigeria. International Journal of Biological and Chemical Sciences. 2014; 8: 1249-1253.
Onyenekwe CC, Ezeani MC, Udeogu AN, Anyiam CD, Melude US, Nnadozie O. Effect of pre and post Academic Examination stress on serum level of cortisol and progesterone circulation amongst students of Nnamdii Azikiwe University Nnewi Campus, Anambra state, Nigeria. International Journal of Tropical Disease and Health. 2012; 4: 62- 69.
Golzari Z, Shabkhiz F, Soudi S, Kordi MR, Hashemi SM. Combined exercise training increase IFN gamma and reduce IL-12 level in the plasma and supernatant of peripheral blood mononuclear cell in women. International Immunopharmacology Journal. 2010; 10: 1415- 1419.
Vanessa N and Elizabeth D. Submaximal exercise testing: Clinical application and interpretation. Journal of the American Physical Therapy Association. 2000; 80: 782- 807.
Ogwumike OO, Arowojolu AO, Sanya AO. Effects of a 12- week endurance exercise program on adiposity and flexibility of Nigerian perimenopausal and postmenopausal women. Nigerian Journal of Physiology Sciences. 2011; 26: 199- 206.
Ehiaghe FA, Ehiaghe IJ, Igere EB, Iyen IR. The effects of aqueous extracts of Acalypha wilkesiana supplementation and exercise training on hematopoietic system in rats. American Journal of Plant Science. 2013; 4: 1834- 1838.
Carl AB and Edward RA. Tietz fundamental of clinical chemistry 5th edition. Saunder, Philadelphia, Pennsylvania. 2006; PP. 432- 437.
Pedersen BK. American Physiological Society. Comprehensive Physiology. 2013; 3:1337-1362.
Denguezli-Bouzgarrou M, Ben-Jabrallah M, Gaid S, Slama F, Ben-Saad H, Tabka Z. Effects of brief maximal exercise on interleukin -6 and tumor necrosis factor alpha. Biology of Sport. 2006; 23: 3-15.
Flaster H, Bernhagen J, Calandra T, Bucala R. The Macrophage Migration Inhibitory Factor-Glucocorticoid Dyad: Regulation of Inflammation and Immunity. Molecular Endocrinology. 2007; 21 (6): 1267-1280.
Xiao W, Chen P, Dong J. Effect of overtraining on skeletal muscle growth and gene expression. International Journal of Sports Medicine. 2012; 33: 846- 853.
Ambarish V and Radhika K. Alteration of interferon gamma in Human plasma with graded physical activity. Journal of Clinical Diagnosis. 2014; 8: 5- 7.
Bente KP. The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. The Biochemical Society. 2006; 42: 105- 117.
Rodrigo T, Silvia AC, Veronica SP, Patricia MLD. Effect of exercise on the immune sytem: response, adapation and cell signaling. Exercise and Sport Science. 2012; 18: 34- 49.
Evans WJ and Cannon JG. The metabolic effects of exercise-induced muscle damage. Exercise and Sport Sciences Reviews. 1991; 19:99-125.