Helicobacter pylori Infection: Seroprevalence and Detection of H. Pylori IgG by Using ELISA
International Journal of Immunology
Volume 3, Issue 2, April 2015, Pages: 21-26
Received: Apr. 12, 2015; Accepted: Apr. 16, 2015; Published: Apr. 24, 2015
Views 3326      Downloads 114
Authors
Sarah Yousef Ahmed, Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt; Department of Clinical Laboratory Sciences, College of Applied Medical Science, University of Hail, Hail, Saudi Arabia
Hesa Nazel Al Shammari, Department of Clinical Laboratory Sciences, College of Applied Medical Science, University of Hail, Hail, Saudi Arabia
Article Tools
Follow on us
Abstract
Background: Helicobacter pylori (H. pylori), one of the most common bacterial pathogens of humans, colonizes the gastric mucosa. H. pylori is a major factor in inflammatory and malignant diseases of the gastrointestinal tract, where it appears to persist throughout the host's life unless the patient is treated. Materials and methods: A study population was carried out through 100 patients who had gastrointestinal symptoms in Hail region. Seroprevalence of Helicobacter pylori was carried out by detection of H. pylori IgG in patient serum by using Enzyme Linked Immunosorbant Assay (ELISA). Results: Among the patients, H. pylori positivity percentage was found to be (57%) (57/100) in our study. It was found that, the average age of the patient was (one to 70 years old), 68% of them were male and 32% female, the majority came from Hail city. Conclusion: high H.pylori positivity ration (57%), that obtained from these study indicates that H.pylori infection is still a common problem among people in Hail region-Saudi Arabia. The titer of IgG antibody to H.pylori in patient serum can be used as non-invasive tests for the presence of gastric H.pylori infection and gastritis.
Keywords
Helicobacter pylori, IgG, Seroprevalence, ELISA
To cite this article
Sarah Yousef Ahmed, Hesa Nazel Al Shammari, Helicobacter pylori Infection: Seroprevalence and Detection of H. Pylori IgG by Using ELISA, International Journal of Immunology. Vol. 3, No. 2, 2015, pp. 21-26. doi: 10.11648/j.iji.20150302.12
References
[1]
Hunt, RH. : Helicobacter pylori: from theory to practice. Proceedings of a symposium. Am J Med,1996; 100 (5A) supplement.
[2]
Soll, AH. : Medical treatment of peptic ulcer disease. Practice guidelines. [Review]. JAMA 1996, 275:622-629.
[3]
Moayyedi P, Axon AT, Feltbower R, Duffett S, Crocombe W, Braunholtz D, Richards IDG, Dowell AC and Forman D : Relation of adult lifestyle and socioeconomic factors to the prevalence of Helicobacter pylori infection. Int J Epidemiol 2002,, 31:624–631.
[4]
Danesh, J. and Peto, R.: Risk factors for coronary heart disease and infection with Helicobacter pylori: meta-analysis of 18 studies. BMJ 1998,316:1130–1132.
[5]
Mati Moyat and Dominique Velin,: Immune responses to Helicobacter pylori infection. World J Gastroenterol 2014, 20(19): 5583–559.
[6]
Richard A.: Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 1999,397, 176-180.
[7]
Marshall, B.J., H. Royce, D.I. Annear et al.,: Helicobacter pylori IgG antibodies in Serum by Enzyme Immunoassay. Helicobacter pylori in Serum NHANES1984, 1999-2000 171-2.
[8]
Thomas JE.: Helicobacter pylori colonization in early life. Pediatric Research J 1999,; 45: 218- 223.
[9]
Perez-Perez GI, Rothenbacher D, Brenner H : Epidemiology of Helicobacter pylori infection. Helicobacter 2004, 9(Suppl 1):1–6.
[10]
Kim SY, Ahn JS, Ha YJ, Doh HJ, Jang MH, Chung SI, Park HJ : Serodiagnosis of Helicobacter pylori infection in Korean patients using enzyme-linked immunosorbent assay. J Immunoassay 1998, 19:251–270.
[11]
Shiota S, Murakami K, Fujioka T, Yamaoka Y : Population-based strategies for Helicobacter pylori-associated disease management: a Japanese perspective. Expert Rev Gastroenterol Hepatol,2010, 4:149–156.
[12]
Suerbaum S, and Michetti P. : Helicobacter pylori infection. NEJM;2002, 347: 1175-1186.
[13]
Brown, L.M.,Thomas, T.L., Ma,J.L. et al.,: H.pylori infection in rural china: demographic, lifestyle and environmental actors. Int. J. Epidemiol.,2002, 31,638-645.
[14]
Ozden, A., Bozdayi, G., Ozkan, M. et al.,: Changes in the seroepidemiological pattern of H.pylori infection over the last 10 years in Turkey. Turk. J. Gastroenterol.,2004, 15,156-158.
[15]
Malaty,H.M., Paykov,V., Bykova,O., et al.,: H.pylori and socioeconomic factors in Russia.Helicobacter,1996, 1,82-87.
[16]
Sninch,K.; Ishii,H.; Imanshi,Ki et al.,: Relation of cigarette smoking, alcohol use and dietary habits H.pylori infection in Japanese men. Scand j. Gastroenterol., 1997, 32, 651-655.
[17]
Triantafyllopoulow,M., Carroll, M. and Li, B.: Helicobacter pylori infection.2006, Online at http://uvwww.emedicine.com/ped/topic 938.htmaccessed 10 January 2015.
[18]
Brow,L.M.: Helicobacter pylori: epidemiology and routes of transmission. Epidemiol.Rev.,2000,22,283-297.
[19]
Hoffmann,K.M.; Eherer, A. and Kries, G.I.: Arw dyspeptic symptoms linked to H.pylori? A prospective cohort study among medical students. Wienklin.Wochenschri.,2003,115,175-178.
[20]
Jones DM, Eldridge J, Fox AJ, Sethi P, Whorwell PJ. : Antibody to the gastric campylobacter-like organism ("Campylobacter pyloridis")-clinical correlations and distribution in the normal population. Y Med Microbiol 1986;22:57-62.
[21]
Goodwin CS, Blincow E, Peterson G, Sanderson C, Cheng W, Marshall B, et al.: Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C pyloridis in the gastric mucosa. _JInfect Dis 1987;155:488-94.
[22]
Booth L, Holdstock G, MacBnde H, Hawtin P, Gibson JR, Ireland A, et al.: Clinical importance of Campylo bacter pyloridis and associated serum IgG and IgA antibody responses in patients undergoing upper gastrointestinal endoscopy. Clin Pathol,1986 ;39:215-19.
[23]
Newell DG, Johnston BJ, Ali MH, Reed PI.: An Enzyme Linked Immuno Sorbent Assay for the serodiagnosis of Campylobacter pylori-associated gastritis. Scand Gastroenterol 1988;23 (suppl 142):53-7.
[24]
Testoni, P. A., E. Colombo, L. Cattani, M. Longhi, F. Bagnolo, F. Lella, M. Buizza, and R. Scelsi. Helicobacter pylori serology in chronic gastritis with antral atrophy and negative histology for helicobacter-like organisms. J. Clin. Gastroenterol. 1996. 22:182–185.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186